Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,032,104	T→C	intergenic (‑392/‑35)	serT ← / → hyaA	tRNA‑Ser/hydrogenase 1 small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,032,104	0	T	C	100.0%	146.3 / NA	43	intergenic (‑392/‑35)	serT/hyaA	tRNA‑Ser/hydrogenase 1 small subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/32);  total (11/32)

CTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCAAATATTGTAGTCTGGCTGCCACGTCGCTGGGAT  >  NC_000913/1031970‑1032238                                                                                                                                       |                                                                                                                                       cTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGc                                                                                                                                   <  2:196933/140‑1 (MQ=255)   aCGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCg                                                                                                                                 <  1:541460/140‑1 (MQ=255)    cGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCgg                                                                                                                                <  2:548918/140‑1 (MQ=255)      cTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGgtg                                                                                                                              >  2:590093/1‑140 (MQ=255)           ttaACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  1:80488/140‑1 (MQ=255)           ttaACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  1:87322/140‑1 (MQ=255)           ttaACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  1:590093/140‑1 (MQ=255)           ttaACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  1:295414/140‑1 (MQ=255)           ttaACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  2:586476/140‑1 (MQ=255)           ttaACAATACTTTCTGGCGACGTGCGACAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  1:694036/140‑1 (MQ=255)               cAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAagga                                                                                                                     >  1:322546/1‑140 (MQ=255)                 aTACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         <  1:530889/134‑1 (MQ=255)                 aTACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCa                                                                                                                         >  2:530889/1‑134 (MQ=255)                     tttCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGgagag                                                                                                               >  1:421800/1‑140 (MQ=255)                     tttCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGgagag                                                                                                               >  1:652577/1‑140 (MQ=255)                       tCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGAc                                                                                                             >  1:683368/1‑140 (MQ=255)                        cTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACg                                                                                                            <  1:776143/140‑1 (MQ=255)                         tGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGt                                                                                                           <  2:25158/140‑1 (MQ=255)                          ggCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTg                                                                                                          <  2:701237/140‑1 (MQ=255)                           gCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGc                                                                                                         <  2:284278/140‑1 (MQ=255)                             gACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGa                                                                                                       <  2:652577/140‑1 (MQ=255)                              aCGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGat                                                                                                      <  2:826775/140‑1 (MQ=255)                              aCGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGACGCAAGGAGGAGAGACGTGCGat                                                                                                      <  2:759910/140‑1 (MQ=255)                                           cAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAgg                                                                                         <  2:322546/140‑1 (MQ=255)                                           cAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAgg                                                                                         <  2:728516/140‑1 (MQ=255)                                            aGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGa                                                                                        <  2:777330/140‑1 (MQ=255)                                             gAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGaa                                                                                       <  1:519533/140‑1 (MQ=255)                                                       cTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTAcc                                                                             <  2:289825/140‑1 (MQ=255)                                                          tCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAgg                                                                          >  2:578658/1‑140 (MQ=255)                                                                 cAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATgcg                                                                   >  2:378257/1‑140 (MQ=255)                                                                 cAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATgcg                                                                   <  1:378257/140‑1 (MQ=255)                                                                 cAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATgcg                                                                   >  1:547667/1‑140 (MQ=255)                                                                      tctcACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTc                                                              <  1:380683/140‑1 (MQ=255)                                                                       ctcACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCa                                                             <  1:590797/140‑1 (MQ=255)                                                                                         ttGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAGGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAg                                           <  2:547667/140‑1 (MQ=255)                                                                                         ttGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAg                                           <  2:201673/140‑1 (MQ=255)                                                                                                gCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTtctc                                    <  2:99184/140‑1 (MQ=255)                                                                                                  tACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCaa                                  <  1:377157/140‑1 (MQ=255)                                                                                                  tACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCaa                                  <  2:421800/140‑1 (MQ=255)                                                                                                            tCGCTTGCCGACCATAAGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCAAATATTGTAGt                        >  2:431280/1‑140 (MQ=255)                                                                                                                           aaGCGCCCGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCAAATATTGTAGTCTGGCTGCCACGTCg         <  1:431280/140‑1 (MQ=255)                                                                                                                                  cGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCAAATATTGTAGTCTGGCTGCCACGTCGCTGGGAt  <  2:683368/140‑1 (MQ=255)                                                                                                                                  cGGTGCCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCAAATATTGTAGTCTGGCTGCCACGTCGCTGGGAt  >  2:548690/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CTACGCTTTATTAACAATACTTTCTGGCGACGTGCGCCAGTGCAGAAGGATGAGCTTTCGTTTTCAGCATCTCACGTGAAGCGATGGTTTGCCTTGCTACAGGGACGTCGCTTGCCGACCATAAGCGCCCGGTGTCCTGCCGGTGTCGCAAGGAGGAGAGACGTGCGATATGAATAACGAGGAAACATTTTACCAGGCCATGCGGCGTCAGGGCGTTACCCGGCGCAGCTTTCTCAAATATTGTAGTCTGGCTGCCACGTCGCTGGGAT  >  NC_000913/1031970‑1032238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑