Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,074,846	A→G	Q202R (CAG→CGG)	rutR →	DNA‑binding transcriptional dual regulator RutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,074,846	0	A	G	100.0%	80.4 / NA	25	Q202R (CAG→CGG)	rutR	DNA‑binding transcriptional dual regulator RutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/15);  total (10/15)

TTGATTTTTATGATTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAC  >  NC_000913/1074725‑1074981                                                                                                                          |                                                                                                                                        ttGATTTTTATGATTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAggg                                                                                                                       >  2:517924/1‑140 (MQ=255) ttGATTTTTATGATTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGGATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAggg                                                                                                                       >  2:57294/1‑140 (MQ=255)         tATGATTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGAc                                                                                                               <  1:725757/140‑1 (MQ=255)         tATGATTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGAc                                                                                                               <  2:532532/140‑1 (MQ=255)             aTTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACg                                                                                                           <  1:562439/140‑1 (MQ=255)                        cACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGGATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGcc                                                                                                >  2:614437/1‑140 (MQ=255)                           tCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCgga                                                                                             <  1:484823/140‑1 (MQ=255)                                aTTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGaggagg                                                                                        <  2:647886/140‑1 (MQ=255)                                  tACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGtt                                                                                      >  2:689646/1‑140 (MQ=255)                                         aTTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCTCGTTAAAGATGCCGGAGGAGGGTGTAAGAt                                                                               >  2:509504/1‑140 (MQ=255)                                                   tCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTa                                                                     <  1:57294/140‑1 (MQ=255)                                                        tGGGGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGt                                                                <  1:717278/140‑1 (MQ=255)                                                         ggAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCT‑CGGCTACCTGttt                                                              <  1:517924/140‑1 (MQ=255)                                                          gAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGttt                                                              <  1:509504/140‑1 (MQ=255)                                                           aGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTa                                                             <  1:80064/140‑1 (MQ=255)                                                            ggCGGTGACAGGCGCGACGTTGCGCGATGAGGGATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTaa                                                            >  1:332150/1‑140 (MQ=255)                                                                 tGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCtat                                                       <  2:550333/140‑1 (MQ=255)                                                                         gcgACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTa                                               >  2:741065/1‑140 (MQ=255)                                                                                  cgcgATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGt                                      >  1:251118/1‑140 (MQ=255)                                                                                         aGGTATTTTTCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGcc                               <  1:689646/140‑1 (MQ=255)                                                                                              tttttCAATCAAACGGTTGAAAACGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCcagcgg                          <  2:292435/140‑1 (MQ=255)                                                                                                                   aaCGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCg     >  1:468671/1‑140 (MQ=255)                                                                                                                     cGTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGaa   <  2:332150/140‑1 (MQ=255)                                                                                                                      gTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAc  >  2:388279/1‑140 (MQ=255)                                                                                                                      gTGCGGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAc  <  1:614437/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                        TTGATTTTTATGATTTGGGCTTCCACTCAACATTACGCCGATTTCGCCCCTCAGGTGGAGGCGGTGACAGGCGCGACGTTGCGCGATGAGGTATTTTTCAATCAAACGGTTGAAAACGTGCAGCGGATTATTATTGAGGGGATTCGACCACGTTAAAGATGCCGGAGGAGGTTGTAACATCCTCCGGCTACCTGTTTAACCTATAGTCATTAAGCTGGCGTTACCGCCAGCGGCAGCGGTATTCACACTCAGCGAAC  >  NC_000913/1074725‑1074981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑