Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	154,673	A→G	S252P (TCT→CCT)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,673	0	A	G	100.0%	230.5 / NA	66	S252P (TCT→CCT)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/29);  total (37/29)

ACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATG  >  NC_000913/154537‑154798                                                                                                                                         |                                                                                                                              aCTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTc                                                                                                                            >  1:406420/1‑140 (MQ=255)      ggCGTTGGTATTGGCAACGCCGTGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCaa                                                                                                                       >  1:205625/1‑140 (MQ=255)      ggCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCaa                                                                                                                       >  1:608867/1‑140 (MQ=255)      ggCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCaa                                                                                                                       >  1:246061/1‑140 (MQ=255)        cGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGa                                                                                                                     <  2:165685/140‑1 (MQ=255)        cGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGa                                                                                                                     >  1:674814/1‑140 (MQ=255)          ttGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTGACCAAGAAt                                                                                                                   <  2:278851/140‑1 (MQ=255)            ggTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGa                                                                                                                 >  2:826612/1‑140 (MQ=255)                     aaCGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAAc                                                                                                        >  2:352997/1‑140 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAAt                                                                                                                   <  2:437682/127‑1 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAAt                                                                                                                   >  1:437682/1‑127 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGa                                                                                                                 <  2:633655/129‑1 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGa                                                                                                                 >  1:633655/1‑129 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGc                                                                                                      >  1:342151/1‑140 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGc                                                                                                      <  2:674814/140‑1 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGc                                                                                                      >  2:231868/1‑140 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGc                                                                                                      >  2:103712/1‑140 (MQ=255)                       cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCACCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGc                                                                                                      >  2:815518/1‑140 (MQ=255)                           atgaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCg                                                                                                  <  1:101645/140‑1 (MQ=255)                             gaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAg                                                                                                >  2:31589/1‑140 (MQ=255)                              aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAgg                                                                                               <  1:762383/140‑1 (MQ=255)                                   tAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGata                                                                                          <  1:352997/140‑1 (MQ=255)                                      gcgcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCa                                                                                       >  2:666612/1‑140 (MQ=255)                                        gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                     >  1:209984/1‑140 (MQ=255)                                        gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                     <  1:231868/140‑1 (MQ=255)                                        gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                     >  1:271046/1‑140 (MQ=255)                                        gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                     >  1:773070/1‑140 (MQ=255)                                        gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                     <  1:778451/140‑1 (MQ=255)                                          aaaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCCCCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCt                                                                                   <  2:205625/140‑1 (MQ=255)                                          aaaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCt                                                                                   <  1:666612/140‑1 (MQ=255)                                          aaaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCt                                                                                   <  1:671374/140‑1 (MQ=255)                                          aaaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCt                                                                                   >  2:535767/1‑140 (MQ=255)                                           aaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTg                                                                                  <  1:571839/140‑1 (MQ=255)                                                 gCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATa                                                                            >  2:334455/1‑140 (MQ=255)                                                        tCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTc                                                                     >  2:535718/1‑140 (MQ=255)                                                           gAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTa                                                                  >  1:144503/1‑140 (MQ=255)                                                           gAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTa                                                                  >  2:766554/1‑140 (MQ=255)                                                           gAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTa                                                                  <  1:766554/140‑1 (MQ=255)                                                           gAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTa                                                                  >  1:681001/1‑140 (MQ=255)                                                                 aCATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGCACATACCGATTCTTAAAATCa                                                            <  2:588338/140‑1 (MQ=255)                                                                      cGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAAtt                                                       <  2:608867/140‑1 (MQ=255)                                                                          tGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTg                                                   >  1:651481/1‑140 (MQ=255)                                                                           gTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGc                                                  <  2:651481/140‑1 (MQ=255)                                                                               cTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGa                                              <  2:593688/140‑1 (MQ=255)                                                                                 gTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAAt                                            >  2:149310/1‑140 (MQ=255)                                                                                   ataAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCg                                          >  1:165850/1‑140 (MQ=255)                                                                                    taAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCgg                                         >  1:618453/1‑140 (MQ=255)                                                                                       aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                      <  1:826612/140‑1 (MQ=255)                                                                                       aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                      <  2:209984/140‑1 (MQ=255)                                                                                         gAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATc                                    <  1:535718/140‑1 (MQ=255)                                                                                          aaTGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATcc                                   >  2:115314/1‑140 (MQ=255)                                                                                            tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                      <  1:753324/135‑1 (MQ=255)                                                                                            tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                      >  2:753324/1‑135 (MQ=255)                                                                                            tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                 >  2:434750/1‑140 (MQ=255)                                                                                            tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                 >  2:644764/1‑140 (MQ=255)                                                                                            tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                 >  1:478049/1‑140 (MQ=255)                                                                                            tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                 >  1:432020/1‑140 (MQ=255)                                                                                              ccTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATc                                    <  1:716327/135‑1 (MQ=255)                                                                                              ccTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATc                                    >  2:716327/1‑135 (MQ=255)                                                                                               cTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTg                              <  2:681001/140‑1 (MQ=255)                                                                                                 cGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTa                            >  1:505615/1‑140 (MQ=255)                                                                                                   gATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGt                          <  1:149310/140‑1 (MQ=255)                                                                                                               aTCAAAGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCAc              >  2:224563/1‑140 (MQ=255)                                                                                                                   aaGGTTTCGCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAg          <  1:103712/140‑1 (MQ=255)                                                                                                                           gCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATg  <  2:271046/140‑1 (MQ=255)                                                                                                                           gCCCGTCGTATAAGGCTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATg  <  2:478049/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                              ACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATG  >  NC_000913/154537‑154798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑