Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,158,486	A→G	V206V (GTA→GTG)	ptsG →	glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,158,486	0	A	G	100.0%	146.2 / NA	42	V206V (GTA→GTG)	ptsG	glucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/28);  total (14/28)

CATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAA  >  NC_000913/1158351‑1158615                                                                                                                                        |                                                                                                                                  cATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGt                                                                                                                               <  1:485543/140‑1 (MQ=255)      ttACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGt                                                                                                                          <  2:240502/140‑1 (MQ=255)      ttACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGt                                                                                                                          <  1:490166/140‑1 (MQ=255)      ttACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGt                                                                                                                          <  2:167165/140‑1 (MQ=255)         cTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTg                                                                                                                       <  2:83641/140‑1 (MQ=255)         cTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTg                                                                                                                       <  2:674977/140‑1 (MQ=255)            gCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGcac                                                                                                                    <  1:198669/140‑1 (MQ=255)              gTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGcacca                                                                                                                  <  1:40308/140‑1 (MQ=255)              gTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGcac                                                                                                                    >  2:695209/1‑138 (MQ=255)              gTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGcac                                                                                                                    <  1:695209/138‑1 (MQ=255)                  tgCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATc                                                                                                              <  1:351697/140‑1 (MQ=255)                  tgCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATc                                                                                                              <  1:680351/140‑1 (MQ=255)                            cATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATc                                                                                                              >  2:549326/1‑130 (MQ=255)                            cATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATc                                                                                                              <  1:549326/130‑1 (MQ=255)                                 ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                                                               >  2:171208/1‑140 (MQ=255)                                 ggCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTc                                                                                               >  2:455163/1‑140 (MQ=255)                                           tGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGa                                                                                     <  1:819633/140‑1 (MQ=255)                                           tGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGa                                                                                     <  2:170229/140‑1 (MQ=255)                                              ttCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTg                                                                                  <  2:616838/140‑1 (MQ=255)                                              ttCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTg                                                                                  <  2:360723/140‑1 (MQ=255)                                              ttCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTg                                                                                  <  2:211567/140‑1 (MQ=255)                                                        cAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGt                                                                                >  1:229113/1‑132 (MQ=255)                                                        cAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGt                                                                                <  2:229113/132‑1 (MQ=255)                                                                ctctcAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACgcag                                                                >  2:32797/1‑140 (MQ=255)                                                                     aGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggt                                                           >  1:208279/1‑140 (MQ=255)                                                                         ggCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGcaggtcagg                                                       >  1:70184/1‑140 (MQ=255)                                                                           ctgctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGtt                                                     >  1:512813/1‑140 (MQ=255)                                                                            tgctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACgca                                                                 >  2:24359/1‑127 (MQ=255)                                                                            tgctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACgca                                                                 <  1:24359/127‑1 (MQ=255)                                                                               ttACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTcc                                                 >  2:699820/1‑140 (MQ=255)                                                                                        cccGGTAGTTGCTTTTGGCATTTGCGGTTTCATCGAGCGTTGCCTGGTGCCGTATTGACTGCGCCTCATCTTGAACCTACCTTTACAGATGCAGATAGGTGAATACACCAACGCAGCAGGTCAGGTGTTCCACGGCGCCa                                        <  2:377296/140‑1 (MQ=255)                                                                                          cGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACAtt                                      <  2:348016/140‑1 (MQ=255)                                                                                            gTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTcc                                    <  2:512813/140‑1 (MQ=255)                                                                                               gTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCcgcg                                 <  1:455163/140‑1 (MQ=255)                                                                                                     tttGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTatat                           <  1:244464/140‑1 (MQ=255)                                                                                                     tttGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTatat                           >  2:723516/1‑140 (MQ=255)                                                                                                       tGGCATTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATgg                         <  1:699820/140‑1 (MQ=255)                                                                                                           aTTTACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCggg                     <  1:678193/140‑1 (MQ=255)                                                                                                              tACGGTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGa                  >  2:382045/1‑140 (MQ=255)                                                                                                                  gTTTCATCGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCg              >  2:737135/1‑140 (MQ=255)                                                                                                                         cGAACGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg       <  2:70184/140‑1 (MQ=255)                                                                                                                            aCGTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTa    <  2:208279/140‑1 (MQ=255)                                                                                                                              gTTGCCTGGTGCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaaa  <  1:382045/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CATCTTTACTGGCGTTGTGCTGTCCTTCATTTGGCCGCCGATTGGTTCTGCAATCCAGACCTTCTCTCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAA  >  NC_000913/1158351‑1158615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑