Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	163,921	A→G	D606G (GAC→GGC)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	163,921	0	A	G	100.0%	154.3 / NA	44	D606G (GAC→GGC)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/20);  total (24/20)

CGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCA  >  NC_000913/163788‑164054                                                                                                                                      |                                                                                                                                      cGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGtt                                                                                                                                 >  2:423472/1‑140 (MQ=255) cGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGtt                                                                                                                                 >  2:228/1‑140 (MQ=255)     gCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCAt                                                                                                                             <  1:137238/140‑1 (MQ=255)           aGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCg                                                                                                                       <  1:228/140‑1 (MQ=255)           aGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCg                                                                                                                       <  1:583556/140‑1 (MQ=255)             cTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGAt                                                                                                                     <  1:209457/140‑1 (MQ=255)                gTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCTGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGc                                                                                                                        >  2:352637/1‑134 (MQ=255)                gTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCTGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGc                                                                                                                        <  1:352637/134‑1 (MQ=255)                      cagTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATgg                                                                                                            >  1:129871/1‑140 (MQ=255)                      cagTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATgg                                                                                                            >  1:512486/1‑140 (MQ=255)                       agTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCAt                                                                                                                             <  2:798795/122‑1 (MQ=255)                       agTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCAt                                                                                                                             >  1:798795/1‑122 (MQ=255)                           tGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATcc                                                                                                       >  1:327405/1‑140 (MQ=255)                           tGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATcc                                                                                                       <  2:123047/140‑1 (MQ=255)                           tGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATcc                                                                                                       >  1:355776/1‑140 (MQ=255)                                  gtgGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATaa                                                                                                <  1:47499/140‑1 (MQ=255)                                   tgGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATaaa                                                                                               >  1:471751/1‑140 (MQ=255)                                   tgGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATaaa                                                                                               >  1:644709/1‑140 (MQ=255)                                   tgGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATaaa                                                                                               >  1:644710/1‑140 (MQ=255)                                     gAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAgg                                                                                             >  2:578422/1‑140 (MQ=255)                                        tGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATaa                                                                                                <  1:366465/134‑1 (MQ=255)                                        tGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATaa                                                                                                >  2:366465/1‑134 (MQ=255)                                             tGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCg                                                                                     >  1:251723/1‑140 (MQ=255)                                               aCGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTg                                                                                   >  1:608321/1‑140 (MQ=255)                                                cgcgCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGc                                                                                  >  1:292887/1‑140 (MQ=255)                                                                 aaGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCt                                                                                 >  1:650516/1‑124 (MQ=255)                                                                 aaGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCt                                                                                 <  2:650516/124‑1 (MQ=255)                                                                 aaGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGgcggaa                                                                 >  1:711558/1‑140 (MQ=255)                                                                      tGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGgcggaagcgga                                                            <  1:578422/140‑1 (MQ=255)                                                                      tGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGgcggaagcgga                                                            <  1:423472/140‑1 (MQ=255)                                                                        gCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAAc                                                          >  2:212423/1‑140 (MQ=255)                                                                                  cAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAAc                                                          >  2:348127/1‑130 (MQ=255)                                                                                  cAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAAc                                                          <  1:348127/130‑1 (MQ=255)                                                                                      tCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGt                                            <  2:129871/140‑1 (MQ=255)                                                                                             gTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTAt                                     <  2:608321/140‑1 (MQ=255)                                                                                                     tGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCa                             <  2:251723/140‑1 (MQ=255)                                                                                                           tGCAGCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTgg                       <  2:711558/140‑1 (MQ=255)                                                                                                               gCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGc                   <  2:512486/140‑1 (MQ=255)                                                                                                               gCCGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGc                   >  2:744578/1‑140 (MQ=255)                                                                                                                 cGCTGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCg                 >  1:5562/1‑140 (MQ=255)                                                                                                                    tGGCGAAACCGTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGaa              <  2:292887/140‑1 (MQ=255)                                                                                                                              gTCAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGc    <  2:5562/140‑1 (MQ=255)                                                                                                                                cAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCa  >  1:283939/1‑140 (MQ=255)                                                                                                                                cAGAAGGCGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCa  >  1:467005/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CGCTGCCCGCAGCTGGTACAGCAGTCTGACACTGTGGAGTGGGATGACGCGCAAGGTACGCTGAAAGCCTGGCGTCGGCTACAAATCGGTCAGTTGACGGTGAAAGTGCAGCCGCTGGCGAAACCGTCAGAAGACGAGTTGCATCAGGCGATGCTTAATGGCATCCGTGATAAAGGTTTAAGCGTGCTCAACTGGACGGCGGAAGCGGAACAGCTACGCTTGCGTTTGTTATGCGCCGCAAAGTGGTTGCCGGAATATGACTGGCCA  >  NC_000913/163788‑164054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑