Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,324,648	T→C	V184A (GTT→GCT)	yciO →	putative RNA‑binding protein YciO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,324,648	0	T	C	100.0%	99.9 / NA	30	V184A (GTT→GCT)	yciO	putative RNA‑binding protein YciO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/18);  total (12/18)

ACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGT  >  NC_000913/1324511‑1324776                                                                                                                                          |                                                                                                                                 aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTa                                                                                                                                >  2:629180/1‑140 (MQ=255) aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTa                                                                                                                                >  2:451328/1‑140 (MQ=255) aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTa                                                                                                                                <  1:451328/140‑1 (MQ=255)      tGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGAt                                                                                                                           >  2:334850/1‑140 (MQ=255)           tCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTAc                                                                                                                      <  2:94540/140‑1 (MQ=255)                ttCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCgatg                                                                                                                 <  2:444503/140‑1 (MQ=255)                 tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCgatga                                                                                                                <  2:214454/140‑1 (MQ=255)                    cTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATAc                                                                                                             >  2:662853/1‑140 (MQ=255)                                    gCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGt                                                                                             <  1:629180/140‑1 (MQ=255)                                     cGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTg                                                                                            >  2:798212/1‑140 (MQ=255)                                         tttACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAgg                                                                                        <  1:104062/140‑1 (MQ=255)                                            aCCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGt                                                                                     <  1:798212/140‑1 (MQ=255)                                             ccGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCgga                                                                                    >  1:577960/1‑138 (MQ=255)                                                aaTCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt                                                                                 <  2:566796/140‑1 (MQ=255)                                                           gaagaaATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGcc                                                                      <  2:617472/140‑1 (MQ=255)                                                                      aGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAAc                                                           >  2:557341/1‑140 (MQ=255)                                                                           gTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTAGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAAtt                                                      >  2:422272/1‑140 (MQ=255)                                                                            tCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTg                                                     >  1:771739/1‑140 (MQ=255)                                                                                gAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGtt                                                 <  1:662853/140‑1 (MQ=255)                                                                                gAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAAC‑GCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTc                                                >  2:629897/1‑140 (MQ=255)                                                                                   aaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCa                                              <  1:375141/140‑1 (MQ=255)                                                                                   aaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCa                                              <  2:771739/140‑1 (MQ=255)                                                                                   aaaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAAC‑GCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCAc                                             <  1:629897/140‑1 (MQ=255)                                                                                    aaCAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCAc                                             <  1:334850/140‑1 (MQ=255)                                                                                          tAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATgg                                       <  2:310553/140‑1 (MQ=255)                                                                                           aGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGa                                      <  1:230158/140‑1 (MQ=255)                                                                                                       tCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAg                          <  1:813656/140‑1 (MQ=255)                                                                                                       tCACGGCGGCTATCTCGGGCAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAg                          >  1:798244/1‑140 (MQ=255)                                                                                                                          cAGAAACCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCAtttt       <  1:590290/140‑1 (MQ=255)                                                                                                                             aaaCCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCt    >  2:234168/1‑140 (MQ=255)                                                                                                                               aCCGACAACGGCTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGt  >  1:558394/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                 ACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGT  >  NC_000913/1324511‑1324776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑