Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,329,681 T→C V117A (GTG→GCG)  sohB → S49 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,329,6810TC93.1% 85.4 / ‑2.1 29V117A (GTG→GCG) sohBS49 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (12/15);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCT  >  NC_000913/1329548‑1329813
                                                                                                                                     |                                                                                                                                    
aaaGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTc                                                                                                                                >  2:354854/1‑140 (MQ=255)
  aGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGc                                                                                                                              <  2:613037/140‑1 (MQ=255)
       agaagaAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGt                                                                                                                         <  1:286306/140‑1 (MQ=255)
        gaagaaAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTg                                                                                                                        <  2:29533/140‑1 (MQ=255)
                 gCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATaa                                                                                                               <  2:358581/140‑1 (MQ=255)
                    tAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACgg                                                                                                            <  1:524783/140‑1 (MQ=255)
                      aGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCTGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCt                                                                                                          <  2:304856/140‑1 (MQ=255)
                            aagCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTc                                                                                                    >  1:528904/1‑140 (MQ=255)
                               cGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCgca                                                                                                 >  1:404003/1‑140 (MQ=255)
                                      agcaAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCa                                                                                          >  2:97225/1‑140 (MQ=255)
                                       gcaAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCaa                                                                                         <  1:354854/140‑1 (MQ=255)
                                           aaGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCg                                                                                     <  2:554497/140‑1 (MQ=255)
                                             gCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCa                                                                                   <  2:528904/140‑1 (MQ=255)
                                                aaaGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGa                                                                                <  2:153980/140‑1 (MQ=255)
                                                             gCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTc                                                                   >  1:423781/1‑140 (MQ=255)
                                                                                   aCCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgca                                             <  1:759984/140‑1 (MQ=255)
                                                                                   aCCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgca                                             <  1:782029/140‑1 (MQ=255)
                                                                                    ccccGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcat                                            >  2:717219/1‑140 (MQ=255)
                                                                                          gTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcatggtgca                                      <  2:510768/140‑1 (MQ=255)
                                                                                          gTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgca                                             >  2:73930/1‑133 (MQ=255)
                                                                                          gTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGgca                                             <  1:73930/133‑1 (MQ=255)
                                                                                              gggTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCATGTTGTGCTCCGTCTGGAAAGCCCTGGTGgcatggtgcatggt                                  >  2:58423/1‑140 (MQ=255)
                                                                                                    tGGATTTTAAAGGCAGCATGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACggg                            >  1:381968/1‑140 (MQ=255)
                                                                                                                  agcaTGGACGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCa              >  1:662471/1‑140 (MQ=255)
                                                                                                                          cGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTggcggc                    <  1:548993/126‑1 (MQ=255)
                                                                                                                          cGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTggcggc                    <  1:384734/126‑1 (MQ=255)
                                                                                                                          cGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTggcggc                    >  2:384734/1‑126 (MQ=255)
                                                                                                                          cGCCCATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTggcggc                    >  2:548993/1‑126 (MQ=255)
                                                                                                                              cATGAAGCGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCt  >  2:506372/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                    
AAAGCGCAGAAGAAAAAGCATAAGCAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCCCGCGTCTGGGTGCTGGATTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCTGTACTCGCAGCATTCAAACCGCAGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGCTGGCGGCTTCGCAACTGCAGCGTCT  >  NC_000913/1329548‑1329813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑