Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,334	T→C	S168S (AGT→AGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,334	0	T	C	100.0%	119.8 / NA	35	S168S (AGT→AGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/19);  total (16/19)

GTCGACCTGGTCATTGACCACTCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTC  >  NC_000913/1336197‑1336468                                                                                                                                          |                                                                                                                                       gTCGACCTGGTCATTGACCACTCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCg                                                                                                                                      >  2:546624/1‑140 (MQ=255)              ttGACCACTCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTg                                                                                                                         <  1:546624/140‑1 (MQ=255)                gACCACTCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTgcc                                                                                                                       >  1:21293/1‑140 (MQ=255)                gACCACTCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTgcc                                                                                                                       >  1:649141/1‑140 (MQ=255)                      tCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCcagg                                                                                                                 <  1:167785/140‑1 (MQ=255)                       cGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGTGTCGTGCCGCcaggc                                                                                                                >  1:514663/1‑140 (MQ=255)                          tGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCcaggcaca                                                                                                             >  2:680238/1‑140 (MQ=255)                            aCCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCcaggcacagg                                                                                                           <  1:782820/140‑1 (MQ=255)                               gTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCAt                                                                                                        <  2:79825/140‑1 (MQ=255)                               gTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCAt                                                                                                        <  1:471062/140‑1 (MQ=255)                                 cGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCAttt                                                                                                      <  2:31246/140‑1 (MQ=255)                                      gTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCAt                                                                                                 <  1:165770/140‑1 (MQ=255)                                           ggTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGt                                                                                            <  2:21293/140‑1 (MQ=255)                                           ggTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGt                                                                                            <  2:649141/140‑1 (MQ=255)                                                           ttGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATAtctc                                                                            >  2:473696/1‑140 (MQ=255)                                                                        aCGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAgtgt                                                               >  1:370331/1‑140 (MQ=255)                                                                                     gAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCa                                                  <  2:370331/140‑1 (MQ=255)                                                                                     gAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCa                                                  <  1:680238/140‑1 (MQ=255)                                                                                               aCGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGaa                                        <  2:87375/140‑1 (MQ=255)                                                                                                            gtTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTg                                 >  2:343057/1‑134 (MQ=255)                                                                                                            gtTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTg                                 <  1:343057/134‑1 (MQ=255)                                                                                                                  gAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATa                     >  1:484063/1‑140 (MQ=255)                                                                                                                   aaaTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATac                    >  1:489677/1‑140 (MQ=255)                                                                                                                   aaaTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATac                    >  1:31654/1‑140 (MQ=255)                                                                                                                    aaTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATaca                   <  2:223353/140‑1 (MQ=255)                                                                                                                     aTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTAt                            <  1:207559/130‑1 (MQ=255)                                                                                                                     aTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTAt                            >  2:207559/1‑130 (MQ=255)                                                                                                                     aTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATacac                  >  1:332699/1‑140 (MQ=255)                                                                                                                        gggAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCg               >  2:301787/1‑140 (MQ=255)                                                                                                                         ggAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGt              <  1:473696/140‑1 (MQ=255)                                                                                                                                  gcGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGa     >  1:479944/1‑140 (MQ=255)                                                                                                                                   cGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGAc    <  2:484063/140‑1 (MQ=255)                                                                                                                                    gTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACt   <  2:332699/140‑1 (MQ=255)                                                                                                                                     ttCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTc  >  1:6381/1‑140 (MQ=255)                                                                                                                                       cAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTa         <  2:341720/131‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GTCGACCTGGTCATTGACCACTCGGTGACCGTCGATCGTTTTGGTGATGATGAGGCATTTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTC  >  NC_000913/1336197‑1336468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑