Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,381	A→G	N184S (AAC→AGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,381	0	A	G	100.0%	114.7 / NA	34	N184S (AAC→AGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

TTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGT  >  NC_000913/1336255‑1336517                                                                                                                               |                                                                                                                                         ttGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATAtctc                                                                                                                             >  2:473696/1‑140 (MQ=255)              aCGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAgtgt                                                                                                                >  1:370331/1‑140 (MQ=255)                           gAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCa                                                                                                   <  2:370331/140‑1 (MQ=255)                           gAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCa                                                                                                   <  1:680238/140‑1 (MQ=255)                                     aCGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGaa                                                                                         <  2:87375/140‑1 (MQ=255)                                                  gtTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTg                                                                                  >  2:343057/1‑134 (MQ=255)                                                  gtTCCTGAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTg                                                                                  <  1:343057/134‑1 (MQ=255)                                                        gAAATGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATa                                                                      >  1:484063/1‑140 (MQ=255)                                                         aaaTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATac                                                                     >  1:31654/1‑140 (MQ=255)                                                         aaaTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATac                                                                     >  1:489677/1‑140 (MQ=255)                                                          aaTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATaca                                                                    <  2:223353/140‑1 (MQ=255)                                                           aTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTAt                                                                             >  2:207559/1‑130 (MQ=255)                                                           aTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTAt                                                                             <  1:207559/130‑1 (MQ=255)                                                           aTGGGGAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATacac                                                                   >  1:332699/1‑140 (MQ=255)                                                              gggAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCg                                                                >  2:301787/1‑140 (MQ=255)                                                               ggAAAGCAAGCGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGt                                                               <  1:473696/140‑1 (MQ=255)                                                                        gcGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGa                                                      >  1:479944/1‑140 (MQ=255)                                                                         cGTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGAc                                                     <  2:484063/140‑1 (MQ=255)                                                                          gTTCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACt                                                    <  2:332699/140‑1 (MQ=255)                                                                           ttCAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTc                                                   >  1:6381/1‑140 (MQ=255)                                                                             cagccGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTa                                                          >  1:341720/5‑131 (MQ=255)                                                                             cAGCCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTa                                                          <  2:341720/131‑1 (MQ=255)                                                                              agccGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGca                                                >  2:342912/4‑140 (MQ=255)                                                                                ccGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGcaca                                              <  2:643061/139‑1 (MQ=255)                                                                                ccGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGcaca                                              <  1:342912/139‑1 (MQ=255)                                                                                          gtcgtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGAt                                    >  2:617025/1‑140 (MQ=255)                                                                                             gtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCaa                                 >  1:564311/1‑140 (MQ=255)                                                                                             gtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCaa                                 <  2:479944/140‑1 (MQ=255)                                                                                                     aggcacaggcaTTTGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTg                         <  1:617025/140‑1 (MQ=255)                                                                                                                  tGCCATCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCtggggtg            <  2:514663/140‑1 (MQ=255)                                                                                                                      aTCAGGTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGc        <  1:301787/140‑1 (MQ=255)                                                                                                                           gTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGc        <  2:399817/135‑1 (MQ=255)                                                                                                                           gTTAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGc        >  1:399817/1‑135 (MQ=255)                                                                                                                            ttAGCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTtggt  <  2:6381/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         TTGAAGAAAACGTACGCCTGGAAATGGAGCGCAACCACGAACGTTATGTGTTCCTGAAATGGGGAAAGCAAGCGTTCAGTCGGTTTAGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGT  >  NC_000913/1336255‑1336517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑