Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,348,051	G→A	L288F (CTC→TTC)	rnb ←	RNase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,348,051	0	G	A	100.0%	118.3 / NA	35	L288F (CTC→TTC)	rnb	RNase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/16);  total (19/16)

ATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAGCGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAATTTTCGCGGCTTT  >  NC_000913/1347915‑1348186                                                                                                                                         |                                                                                                                                        aTTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGt                                                                                                                                      >  2:217836/1‑140 (MQ=255)   tGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCa                                                                                                                                    <  1:413004/140‑1 (MQ=255)     cTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATg                                                                                                                                  <  1:357526/140‑1 (MQ=255)      ttCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGc                                                                                                                                 <  1:217836/140‑1 (MQ=255)        cACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCgg                                                                                                                               <  1:547474/140‑1 (MQ=255)        cACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCgg                                                                                                                               <  1:335811/140‑1 (MQ=255)              cAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGcc                                                                                                                         >  1:495416/1‑140 (MQ=255)                   tGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTAc                                                                                                                    >  2:55723/1‑140 (MQ=255)                     ccAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCg                                                                                                                  >  1:227802/1‑140 (MQ=255)                         tCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCg                                                                                                              <  2:227802/140‑1 (MQ=255)                                         gccagTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCg                                                                                                              >  1:157717/1‑124 (MQ=255)                                         gccagTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCg                                                                                                              <  2:157717/124‑1 (MQ=255)                                          ccagTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCg                                                                                                              >  2:804862/1‑123 (MQ=255)                                          ccagTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCg                                                                                                              <  1:804862/123‑1 (MQ=255)                                          ccagTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCa                                                                                             <  2:538445/140‑1 (MQ=255)                                          ccagTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCa                                                                                             >  2:481184/1‑140 (MQ=255)                                                                   aGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTc                                                                    >  1:750385/1‑140 (MQ=255)                                                                   aGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTc                                                                    >  2:806132/1‑140 (MQ=255)                                                                    gctttgctttGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTcg                                                                   <  1:481184/140‑1 (MQ=255)                                                                    gctttgctttGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTcg                                                                   >  2:529495/1‑140 (MQ=255)                                                                     ctttgctttgGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTcgc                                                                  <  1:806132/140‑1 (MQ=255)                                                                        tgctttgGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAg                                                               >  2:327711/1‑140 (MQ=255)                                                                        tgctttgGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAg                                                               >  1:726670/1‑140 (MQ=255)                                                                         gctttgGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAgg                                                              <  1:327711/140‑1 (MQ=255)                                                                                  tCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGTGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAgg                                                     <  2:726670/140‑1 (MQ=255)                                                                                            cggcAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAg                                           >  2:609824/1‑140 (MQ=255)                                                                                                aaaGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAg                                       >  1:534993/1‑140 (MQ=255)                                                                                                           tattatCTTCAATGGTGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTg                            >  1:169690/1‑140 (MQ=255)                                                                                                                          tGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAAtt             >  2:180875/1‑140 (MQ=255)                                                                                                                          tGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAAtt             >  2:333047/1‑140 (MQ=255)                                                                                                                          tGCCATCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAAtt             >  2:668136/1‑140 (MQ=255)                                                                                                                              aTCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAATTTTcg         <  2:750385/140‑1 (MQ=255)                                                                                                                              aTCAGCGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTACATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAATTTTcg         <  2:769360/140‑1 (MQ=255)                                                                                                                                   cGGAGAACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAATTTTCGCGGCt    >  1:695367/1‑140 (MQ=255)                                                                                                                                     gagaACGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAATTTTCGCGGCttt  <  1:180875/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ATTGCTTCACTTTCAGGCTGCCAGTCACCGGTATTTTCCAGCCAGTCAGAAACCTGGTCATACACCAGCTTTGCTTTGGATTCGATGGTGGCGGCAAAGAATTCGATATTATCTTCAATGGTGCCATCAGCGGAGAGCGTCATGCGGCATGCCAGTACCGGGCGGACTTCATTGGCGCGCAGTGAGCAGAGATCGTCAGAAAGCTCGCGAGGCAGCATAGGGATGTTGAAGCCAGGCAGATAGTTGGTGAATGCGCGAATTTTCGCGGCTTT  >  NC_000913/1347915‑1348186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑