Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,269	C→T	A307T (GCT→ACT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,269	0	C	T	100.0%	147.3 / NA	43	A307T (GCT→ACT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

CGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAG  >  NC_000913/1358135‑1358404                                                                                                                                       |                                                                                                                                        cgcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggc                                                                                                                                    >  1:24696/1‑140 (MQ=255)  gcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggcc                                                                                                                                   <  2:24696/140‑1 (MQ=255)  gcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggcc                                                                                                                                   <  1:238567/140‑1 (MQ=255)  gcgACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggcc                                                                                                                                   <  1:806741/140‑1 (MQ=255)    gACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggccag                                                                                                                                 <  1:486556/140‑1 (MQ=255)          cccGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTa                                                                                                                           <  2:442462/140‑1 (MQ=255)            cGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAAc                                                                                                                         <  1:567006/140‑1 (MQ=255)              aCCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggcca                                                                                                                                  <  1:818592/129‑1 (MQ=255)              aCCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAgtcaggcca                                                                                                                                  >  2:818592/1‑129 (MQ=255)              aCCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACgt                                                                                                                       <  2:736233/140‑1 (MQ=255)                   aaTGACGGTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTca                                                                                                                  >  1:737582/1‑140 (MQ=255)                         gTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACg                                                                                                            >  2:601143/1‑140 (MQ=255)                             aGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACgtggt                                                                                                        >  2:703961/1‑140 (MQ=255)                             aGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACgtggt                                                                                                        >  2:626231/1‑140 (MQ=255)                                gCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCa                                                                                                     <  2:406897/140‑1 (MQ=255)                                       tCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACg                                                                                                            >  2:281437/1‑126 (MQ=255)                                       tCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACg                                                                                                            <  1:281437/126‑1 (MQ=255)                                          gCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATc                                                                                           <  2:112463/140‑1 (MQ=255)                                                   ggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaaa                                                                                             >  1:287530/1‑129 (MQ=255)                                                   ggTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGaaaa                                                                                             <  2:287530/129‑1 (MQ=255)                                                        acaTAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCtt                                                                             >  1:54264/1‑140 (MQ=255)                                                        acaTAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCtt                                                                             >  2:451572/1‑140 (MQ=255)                                                                 aaCACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCt                                                                              >  1:26343/1‑130 (MQ=255)                                                                 aaCACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCt                                                                              <  2:26343/130‑1 (MQ=255)                                                                       cgcTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAg                                                              >  1:649061/1‑140 (MQ=255)                                                                        gcTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAgc                                                             >  2:112499/1‑140 (MQ=255)                                                                         cTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAgcg                                                            <  2:628921/140‑1 (MQ=255)                                                                         cTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAgcg                                                            <  1:536452/140‑1 (MQ=255)                                                                                      ttGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAg                                               >  1:179104/1‑140 (MQ=255)                                                                                        gTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTg                                             >  1:203352/1‑140 (MQ=255)                                                                                          cACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCg                                           <  1:749131/140‑1 (MQ=255)                                                                                          cACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCg                                           <  1:703961/140‑1 (MQ=255)                                                                                          cACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCg                                           <  1:601143/140‑1 (MQ=255)                                                                                             gCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCa                                        >  2:617174/1‑140 (MQ=255)                                                                                             gCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCa                                        >  2:495746/1‑140 (MQ=255)                                                                                                       aTACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCtt                              >  2:576606/1‑140 (MQ=255)                                                                                                       aTACAGCAGACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCtt                              <  1:521476/140‑1 (MQ=255)                                                                                                           agcagACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTaaa                          >  2:672492/1‑140 (MQ=255)                                                                                                             cagACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGc                        >  2:124006/1‑140 (MQ=255)                                                                                                             cagACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGc                        >  2:700066/1‑140 (MQ=255)                                                                                                             cagACGCGACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGc                        >  2:625487/1‑140 (MQ=255)                                                                                                                 cgcgACACGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCt                    >  2:150831/1‑140 (MQ=255)                                                                                                                      cacGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGatat               <  1:576606/140‑1 (MQ=255)                                                                                                                       acGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATc              <  1:358375/140‑1 (MQ=255)                                                                                                                       acGCTGGCATGTGAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATc              >  2:678790/1‑140 (MQ=255)                                                                                                                                tgtgAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGaa     <  1:451572/140‑1 (MQ=255)                                                                                                                                tgtgAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGaa     <  1:124006/140‑1 (MQ=255)                                                                                                                                  tgAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACa   <  2:649061/140‑1 (MQ=255)                                                                                                                                   gAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAg  <  1:678790/140‑1 (MQ=255)                                                                                                                                   gAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAg  <  1:625487/140‑1 (MQ=255)                                                                                                                                   gAAGTCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAg  <  1:150831/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAG  >  NC_000913/1358135‑1358404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑