Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,029	A→G	N374S (AAT→AGT)	ycjN →	putative ABC transporter periplasmic binding protein YcjN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,029	0	A	G	100.0%	119.0 / NA	35	N374S (AAT→AGT)	ycjN	putative ABC transporter periplasmic binding protein YcjN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

TGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAG  >  NC_000913/1372899‑1373164                                                                                                                                   |                                                                                                                                        tGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGt                                                                                                                                >  2:75234/1‑140 (MQ=255) tGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGt                                                                                                                                >  2:693014/1‑140 (MQ=255) tGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGt                                                                                                                                >  2:167919/1‑140 (MQ=255) tGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGt                                                                                                                                >  2:18055/1‑140 (MQ=255)               gCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAg                                                                                                                  <  1:75234/140‑1 (MQ=255)               gCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAg                                                                                                                  <  1:18055/140‑1 (MQ=255)               gCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCAAAGTATTCAGGTTTTTGGCGCAGTAg                                                                                                                  >  2:492091/1‑140 (MQ=255)                                  gTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCg                                                                                               <  1:167919/140‑1 (MQ=255)                                               tggtgACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGAtgtg                                                                                  >  1:283632/1‑140 (MQ=255)                                                 gtgACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGAc                                                                                <  2:247342/140‑1 (MQ=255)                                                         cGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTg                                                                        >  1:131549/1‑140 (MQ=255)                                                         cGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCAAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTg                                                                        >  1:3137/1‑140 (MQ=255)                                                              ccTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCgtg                                                                   <  1:320158/140‑1 (MQ=255)                                                              ccTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCgtg                                                                   >  1:830127/1‑140 (MQ=255)                                                                tGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGTGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCgtggt                                                                 <  1:565359/140‑1 (MQ=255)                                                                   aaaGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAg                                                              >  1:109867/1‑140 (MQ=255)                                                                         aaCGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAAt                                                        <  1:740200/140‑1 (MQ=255)                                                                          acgacgTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCAAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATg                                                       <  1:492091/140‑1 (MQ=255)                                                                               gTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCa                                                  <  2:495258/140‑1 (MQ=255)                                                                                     aaGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGt                                            <  2:283632/140‑1 (MQ=255)                                                                                        gcgcTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCAc                                         >  2:309151/1‑140 (MQ=255)                                                                                         cgcTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCAcc                                        <  1:555817/140‑1 (MQ=255)                                                                                           cTGGGTGAACTACCGAATCAGCTAATCGGTGTACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGt                                      >  2:225862/1‑140 (MQ=255)                                                                                           cTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGTGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGt                                      >  2:666319/1‑140 (MQ=255)                                                                                           cTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGt                                      >  1:559169/1‑140 (MQ=255)                                                                                            tGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTg                                     >  2:18759/1‑140 (MQ=255)                                                                                                     tACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGcc                            <  2:131549/140‑1 (MQ=255)                                                                                                     tACCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGcc                            <  1:309151/140‑1 (MQ=255)                                                                                                      aCCGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCg                           >  1:677763/1‑140 (MQ=255)                                                                                                        cGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAt                         <  1:693014/140‑1 (MQ=255)                                                                                                        cGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAt                         <  1:18759/140‑1 (MQ=255)                                                                                                        cGAATCAGCTAATCGGTGAACTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGAt                         <  2:830127/140‑1 (MQ=255)                                                                                                                          aaCTGCCAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCaa       >  1:203886/1‑140 (MQ=255)                                                                                                                               ccAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAg  <  1:225862/140‑1 (MQ=255)                                                                                                                               ccAAGTATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAg  <  2:677763/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGTTTTTGGCGCAGTAGGGGATAAAAACTTTACCCGCATGGGTGATGTGACGGGTTCTGGCGTGGTGAGTTCAATGGTGCATAACGTCACCGTGGGTAAAGCCGATCTCTCTACTACGCTGCAAGCGAG  >  NC_000913/1372899‑1373164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑