Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,376,293	C→T	A87V (GCG→GTG)	ycjR →	putative ketohexose‑3‑epimerase YcjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,293	0	C	T	100.0%	75.5 / NA	23	A87V (GCG→GTG)	ycjR	putative ketohexose‑3‑epimerase YcjR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/10);  total (13/10)

AAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGC  >  NC_000913/1376167‑1376423                                                                                                                               |                                                                                                                                   aaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTg                                                                                                                       <  1:759143/140‑1 (MQ=255)                aaaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCAtc                                                                                                        <  2:381948/140‑1 (MQ=255)                 aaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTgg                                                                                                                      <  2:579133/125‑1 (MQ=255)                 aaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTgg                                                                                                                      >  1:579133/1‑125 (MQ=255)                    cGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCAtcgtcg                                                                                                    >  1:404331/1‑140 (MQ=255)                         tACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCg                                                                                               <  1:680942/140‑1 (MQ=255)                              gTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGc                                                                                          <  1:285559/140‑1 (MQ=255)                                    accGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg                                                                                    <  2:687100/140‑1 (MQ=255)                                    accGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg                                                                                    <  2:282438/140‑1 (MQ=255)                                    accGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg                                                                                    <  2:404331/140‑1 (MQ=255)                                            cGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTa                                                                            >  2:341843/1‑140 (MQ=255)                                                   tATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCg                                                                     <  2:331655/140‑1 (MQ=255)                                                           atggatTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTAccgc                                                             >  1:572234/1‑140 (MQ=255)                                                                   gCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGAcc                                                     >  1:699573/1‑140 (MQ=255)                                                                        tttATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTcgcc                                                >  1:501493/1‑140 (MQ=255)                                                                                          cTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGa                              <  1:341843/140‑1 (MQ=255)                                                                                                 gCTTAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCaaa                       >  1:280251/1‑140 (MQ=255)                                                                                                   ttAAAGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAAt                     >  1:317917/1‑140 (MQ=255)                                                                                                       aGCAGATCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtg                 >  1:114717/1‑140 (MQ=255)                                                                                                            aTCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtgagtga            >  2:724998/1‑140 (MQ=255)                                                                                                            aTCGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGgtgagtga            >  1:574206/1‑140 (MQ=255)                                                                                                              cGAGCGCATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGAtt          <  2:280251/140‑1 (MQ=255)                                                                                                                  cgcATTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCt      >  1:704495/1‑140 (MQ=255)                                                                                                                     aTTCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTCCCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcg   <  2:556164/140‑1 (MQ=255)                                                                                                                      ttCTCGAAGTGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTgcgc  >  2:360364/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                   AAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACCTTCCGCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGC  >  NC_000913/1376167‑1376423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑