Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,420,357	T→G	intergenic (‑116/‑8)	racR ← / → ydaS	Rac prophage; DNA‑binding transcriptional repressor RacR/Rac prophage; toxin YdaS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,420,357	0	T	G	100.0%	144.9 / NA	42	intergenic (‑116/‑8)	racR/ydaS	Rac prophage; DNA‑binding transcriptional repressor RacR/Rac prophage; toxin YdaS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/15);  total (27/15)

GTCTTTACCACTAAGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGTTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAAGGGGTACGTCAATTGC  >  NC_000913/1420224‑1420491                                                                                                                                      |                                                                                                                                       gTCTTTACCACTAAGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTc                                                                                                                                  <  1:97688/140‑1 (MQ=255)      tACCACTAAGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGa                                                                                                                             >  1:773787/1‑140 (MQ=255)           cTAAGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGaaaaaa                                                                                                                        <  1:470522/140‑1 (MQ=255)              aGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAagag                                                                                                                     <  2:264332/140‑1 (MQ=255)              aGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAagag                                                                                                                     <  2:132614/140‑1 (MQ=255)                             tctcCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTcaag                                                                                                      <  1:763390/140‑1 (MQ=255)                               tcCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTcaagca                                                                                                    >  2:602081/1‑140 (MQ=255)                                ccTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTcaagcaa                                                                                                   >  1:259831/1‑140 (MQ=255)                                     gcgcATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTtg                                                                                              <  1:325168/140‑1 (MQ=255)                                           tacattaGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCtgt                                                                                        >  1:762656/1‑140 (MQ=255)                                                       tCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCa                                                                            >  2:652686/1‑140 (MQ=255)                                                         ccTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAAt                                                                          <  2:773787/140‑1 (MQ=255)                                                                  aCTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATgg                                                                 >  1:639981/1‑140 (MQ=255)                                                                  aCTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATgg                                                                 >  2:135468/1‑140 (MQ=255)                                                                  aCTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATgg                                                                 >  1:724221/1‑140 (MQ=255)                                                                   cTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGc                                                                >  1:59015/1‑140 (MQ=255)                                                                    tGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCt                                                               <  2:639981/140‑1 (MQ=255)                                                                       aTTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAgg                                                            >  2:451389/1‑140 (MQ=255)                                                                        ttAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGc                                                           >  1:85718/1‑140 (MQ=255)                                                                           ggCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCttt                                                        >  1:390546/1‑140 (MQ=255)                                                                           ggCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCttt                                                        >  1:76950/1‑140 (MQ=255)                                                                             cacaGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTa                                                      <  2:259831/140‑1 (MQ=255)                                                                                aGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGgt                                                   >  1:285285/1‑140 (MQ=255)                                                                                  ccTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGgtgt                                                 >  1:675940/1‑140 (MQ=255)                                                                                      ttGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCa                                             >  2:200174/1‑140 (MQ=255)                                                                                            aTTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCa                                       >  2:192301/1‑140 (MQ=255)                                                                                            aTTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCa                                       >  2:812688/1‑140 (MQ=255)                                                                                            aTTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCa                                       >  2:169873/1‑140 (MQ=255)                                                                                              tGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAg                                     >  2:274776/1‑140 (MQ=255)                                                                                                cGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCg                                   >  2:220037/1‑140 (MQ=255)                                                                                                       cGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCa                            >  2:494432/1‑140 (MQ=255)                                                                                                       cGTCGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCa                            <  1:812688/140‑1 (MQ=255)                                                                                                          cGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTGTCACCTCCAAGCGTAAATCaa                           >  2:311795/1‑138 (MQ=255)                                                                                                          cGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTGTCACCTCCAAGCGTAAATCaa                           <  1:311795/138‑1 (MQ=255)                                                                                                          cGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGt                                  >  1:496705/1‑131 (MQ=255)                                                                                                          cGCCTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGt                                  <  2:496705/131‑1 (MQ=255)                                                                                                            ccTAATATTTCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGa                       >  1:810906/1‑140 (MQ=255)                                                                                                                     tCTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAAgggg              <  2:85718/140‑1 (MQ=255)                                                                                                                      cTGTGTGTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAAGGGGt             >  1:421567/1‑140 (MQ=255)                                                                                                                       tgtgtgTTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAAGGGGTa            <  1:169873/140‑1 (MQ=255)                                                                                                                         tgtgtTTTTGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAAGGGGTACg          >  1:714447/1‑140 (MQ=255)                                                                                                                                ttGGAGGTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAA‑GGGTACGTCAATtgc  <  2:59015/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GTCTTTACCACTAAGCATTGCTTAATATTCTCCTATGCGCATTACATTAGGCAATCCCTACCCTTACTGCATTAGGCACAGCCTATTGACAATTGCGTTAGGCGTCGCCTAATATTTCTGTGTGTTTTTGGAGTTCATTCGATGAAAAAAGAGAACTATTCATTCAAGCAAGCTTGTGCTGTTGTCGGTGGGCAATCAGCAATGGCTAGGCTTTTAGGTGTATCACCTCCAAGCGTAAATCAATGGATCAAAGGGGTACGTCAATTGC  >  NC_000913/1420224‑1420491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑