Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,430,254 A→G R402R (AGA→AGG)  stfR → Rac prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,430,2540AG100.0% 62.3 / NA 19R402R (AGA→AGG) stfRRac prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/7);  total (12/7)

CAGCGAAGACATCCGAAACGAACGCGAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGACAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAACGCGCGCCGAGACAGCAGCTAAAC  >  NC_000913/1430118‑1430390
                                                                                                                                        |                                                                                                                                        
cAGCGAAGACATCCGAAACGAACGCGAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCaa                                                                                                                                       >  2:356487/1‑140 (MQ=255)
    gAAGACATCCGAAACGAACGCGAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGt                                                                                                                                   >  2:254105/1‑140 (MQ=255)
                aacgaacgCGAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAaga                                                                                                                       >  1:570883/1‑140 (MQ=255)
                    aacgCGAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGagcag                                                                                                                   >  1:549504/1‑140 (MQ=255)
                        cgAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGcc                                                                                                               <  1:254105/140‑1 (MQ=255)
                              cGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCacgacg                                                                                                         <  2:471516/140‑1 (MQ=255)
                                       cAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACg                                                                                                <  1:728598/140‑1 (MQ=255)
                                          gcgcAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAg                                                                                             >  1:653045/1‑140 (MQ=255)
                                                                               cAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTc                                                        >  1:351379/1‑140 (MQ=255)
                                                                                  ttCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCaga                                                     >  2:711783/1‑140 (MQ=255)
                                                                                    cggcggCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGACAGTGCGACGGCGGCAGCTCAGAGc                                                   >  2:654433/1‑140 (MQ=255)
                                                                                                      catcGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATcc                                 >  2:173832/1‑140 (MQ=255)
                                                                                                         cGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCcgcg                              >  1:234502/1‑140 (MQ=255)
                                                                                                         cGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCcgcg                              >  2:532367/1‑140 (MQ=255)
                                                                                                         cGGCGTCTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCcgcg                              >  2:826676/1‑140 (MQ=255)
                                                                                                               cTGCTTCAAAAGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAAcg                        >  2:25317/1‑140 (MQ=255)
                                                                                                                         aGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAACGCGCGCCgaga              <  2:570883/140‑1 (MQ=255)
                                                                                                                         aGATGAGGCGACCAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAACGCGCGCCgaga              <  2:653045/140‑1 (MQ=255)
                                                                                                                                    ccAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAACGCGCGCCGAGACAGCAGCTaaa   <  2:549504/140‑1 (MQ=255)
                                                                                                                                     cAGGCAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAACGCGCGCCGAGACAGCAGCTAAAc  <  1:711783/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CAGCGAAGACATCCGAAACGAACGCGAAAGCGTCGGAAACAAGCGCAGAATCCTCAAAAACGGCTGCCGCATCGTCAGCCAGTTCGGCGGCGTCATCGGCATCATCGGCGTCTGCTTCAAAAGATGAGGCGACCAGACAAGCGTCAGCAGCGAAGAGCAGCGCCACGACGGCATCCACGAAGGCGACAGAGGCTGCTGGCAGTGCGACGGCGGCAGCTCAGAGCAAAAGTACGGCGGAATCCGCGGCAACGCGCGCCGAGACAGCAGCTAAAC  >  NC_000913/1430118‑1430390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑