| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,445,992 | A→G | Y38C (TAC→TGC) | ydbL → | DUF1318 domain‑containing protein YdbL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,445,992 | 0 | A | G | 94.9% | 111.4 / ‑4.4 | 39 | Y38C (TAC→TGC) | ydbL | DUF1318 domain‑containing protein YdbL |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (16/21); minor base T (2/0); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCG > NC_000913/1445856‑1446119 | gTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTTCTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCgg < 1:52372/140‑1 (MQ=255)gTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCgg < 1:88936/140‑1 (MQ=255)gTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCgg > 2:535284/1‑140 (MQ=255) tAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGGTAGTGAAAAAATTTGCGGt > 2:417511/1‑140 (MQ=255) tCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTg > 1:148321/1‑140 (MQ=255) cTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTgcg < 2:619875/140‑1 (MQ=255) cTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTgcg < 1:587037/140‑1 (MQ=255) ggTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGa > 1:194180/1‑140 (MQ=255) gTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGaa > 2:311249/1‑140 (MQ=255) tgatgatgaAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAAAATTTTGCGGTTATCTGGTTGCGCTGaaa > 2:613597/1‑140 (MQ=255) atgatgaAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGGGAAACATTTTTCGGTTATCTGGTTGCGCTGaaaaa > 2:220145/1‑139 (MQ=255) tgatgaAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACg < 1:535284/140‑1 (MQ=255) tACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACagag < 1:352685/140‑1 (MQ=255) aCTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAgaga < 2:329458/140‑1 (MQ=255) tcttTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAAt > 1:449218/1‑140 (MQ=255) cttTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAAtt > 2:658032/1‑140 (MQ=255) cttTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGGGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAAtt > 1:528614/1‑140 (MQ=255) ttGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGGAAAATTTTTCGGTTATCTGGTTGCGCTGCAAACGGATGCTGAAACAGAGTAATtaa > 2:352685/1‑139 (MQ=255) tgCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGGGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATtagtag > 2:223259/1‑140 (MQ=255) cGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTTCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGcc > 1:488068/1‑140 (MQ=255) cGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGcc < 1:311249/140‑1 (MQ=255) cGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGcc < 1:279894/140‑1 (MQ=255) cGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGGTATTTGGTTGCGCTGAAAACGGATGCTGAAAAAGAGAAATTAATAGcc > 2:798867/1‑140 (MQ=255) gCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCAGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCAGACATTAATGCCg < 1:666518/140‑1 (MQ=255) ggTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGGTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAAc > 1:450338/1‑140 (MQ=255) gcagcaATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTaaa < 1:223259/140‑1 (MQ=255) aTGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTGGGGGAAAAATTTTGCGGGTATCTTGGTGCGCTGGAAACGGATGCTGAAACAGGGAAATTAGTAGCCGACATTAATGCCCAACGCAAAAAGAGt > 2:171443/1‑140 (MQ=255) tAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTAc < 1:170607/140‑1 (MQ=255) tAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTAc < 1:798867/140‑1 (MQ=255) tAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTAc < 1:304154/140‑1 (MQ=255) gCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACcaaca > 1:672987/1‑140 (MQ=255) ttGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACt > 1:119618/1‑140 (MQ=255) aCTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgc > 1:250961/1‑140 (MQ=255) ctGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgcaaa < 1:658032/140‑1 (MQ=255) ctGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgcaaa > 2:666314/1‑140 (MQ=255) tGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGgcaaag > 1:178447/1‑140 (MQ=255) aaGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAaat < 1:171443/140‑1 (MQ=255) aaGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAaat < 1:220145/140‑1 (MQ=255) aaGCCAGAACTCAGGGGCGGGTAGGGGAAACATTTTTCGGGTATCTGGGTGCGCTGGAAACGGAGGCTGAAACAGAGAAATTAGTAGCCGACATTTATGCCGAACGTAAAGCGAGTTACCAACAAATGGCAAAGCaaaaa > 2:201698/1‑139 (MQ=255) ccAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAaataat < 1:759077/140‑1 (MQ=255) cAGAACTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGGTACCAACAACTGGCAAAGCAAAATAAtg > 2:651985/1‑140 (MQ=255) aaCTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATgtgt < 1:449122/140‑1 (MQ=255) cTCAGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCg < 2:488068/140‑1 (MQ=255) aGGGGCGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTa > 2:455673/1‑140 (MQ=255) aGGGGCGGGTAGGGGAAACATTTTGCGGGTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTa > 2:515117/1‑140 (MQ=255) cGGGTAGGTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAgatga < 2:449218/140‑1 (MQ=255) gTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATcgcg > 2:64686/1‑140 (MQ=255) gTGAAACATTTTGCGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATcgcg > 1:521496/1‑140 (MQ=255) | GTAGCGATCTTTTCTGAGGTGATGATGAAGAAAACATTACTTCTTTGTGCGTTTCTTGTTGGGCTGGTAAGCAGCAATGTAATGGCATTGACTCTGGATGAAGCCAGAACTCAGGGGCGGGTAGGTGAAACATTTTACGGTTATCTGGTTGCGCTGAAAACGGATGCTGAAACAGAGAAATTAGTAGCCGACATTAATGCCGAACGTAAAGCGAGTTACCAACAACTGGCAAAGCAAAATAATGTGTCGGTAGATGATATCGCG > NC_000913/1445856‑1446119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |