Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,488,752	T→C	V174A (GTC→GCC)	aldA →	aldehyde dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,488,752	0	T	C	100.0%	95.2 / NA	28	V174A (GTC→GCC)	aldA	aldehyde dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

TCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACTGGGGCGTGGTGAAACCGTTGGGCAAGAACTGGCGGG  >  NC_000913/1488621‑1488884                                                                                                                                    |                                                                                                                                     tCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAAc                                                                                                                              >  2:334237/1‑140 (MQ=255) tCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAAc                                                                                                                              >  1:344525/1‑140 (MQ=255)             aaaTATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTa                                                                                                                  >  1:67944/1‑140 (MQ=255)               atatTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACg                                                                                                                <  1:284804/140‑1 (MQ=255)                tatTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGc                                                                                                               <  2:337983/140‑1 (MQ=255)                   tCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCaa                                                                                                            >  2:747910/1‑140 (MQ=255)                       ttGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACaa                                                                                                        <  2:344525/140‑1 (MQ=255)                         gTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATg                                                                                                      >  1:185941/1‑140 (MQ=255)                                    cgcTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTc                                                                                           >  1:483098/1‑140 (MQ=255)                                    cgcTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTc                                                                                           >  2:297440/1‑140 (MQ=255)                                                   ccGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGAt                                                                            >  1:511490/1‑140 (MQ=255)                                                    cGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgtcg                                                                              <  2:669206/137‑1 (MQ=255)                                                    cGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAAtcgtcg                                                                              >  1:669206/1‑137 (MQ=255)                                                    cGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATg                                                                           >  1:334254/1‑140 (MQ=255)                                                         ttCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATa                                                                      >  2:522422/1‑140 (MQ=255)                                                                   gAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCcgc                                                            >  2:357022/1‑140 (MQ=255)                                                                       ttCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCgcgg                                                        <  1:334237/140‑1 (MQ=255)                                                                         cccGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCgcggcg                                                      >  2:266743/1‑140 (MQ=255)                                                                             ttcttcCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGtt                                                  <  2:483098/140‑1 (MQ=255)                                                                             ttcttcCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGtt                                                  <  1:266743/140‑1 (MQ=255)                                                                                  ccTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAAcc                                             <  2:613817/140‑1 (MQ=255)                                                                                  ccTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAAcc                                             <  2:67944/140‑1 (MQ=255)                                                                                        tGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTAc                                       <  1:522422/140‑1 (MQ=255)                                                                                         gCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACt                                      <  2:185941/140‑1 (MQ=255)                                                                                                      cTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACTgggg                                  >  2:418450/1‑131 (MQ=255)                                                                                                      cTCCCGCTCTTTTGACCGGTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACTgggg                                  <  1:418450/131‑1 (MQ=255)                                                                                                                        gTAATACCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACTGGGGCGTGGTGAAACCGTTGGGCAAGAACTg       <  1:487506/140‑1 (MQ=255)                                                                                                                             aCCATCGCCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACTGGGGCGTGGTGAAACCGTTGGGCAAGAACTGGCggg  <  2:12189/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     TCGTCCAGGAGAAAATATTCTTTTGTTTAAACGTGCGCTTGGTGTGACTACCGGCATTCTGCCGTGGAACTTCCCGTTCTTCCTCATTGCCCGCAAAATGGCTCCCGCTCTTTTGACCGGTAATACCATCGTCATTAAACCTAGTGAATTTACGCCAAACAATGCGATTGCATTCGCCAAAATCGTCGATGAAATAGGCCTTCCGCGCGGCGTGTTTAACCTTGTACTGGGGCGTGGTGAAACCGTTGGGCAAGAACTGGCGGG  >  NC_000913/1488621‑1488884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑