Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,360	Δ1 bp	coding (773/804 nt)	ydjO ←	protein YdjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,357	0	G	.	100.0%	213.6 / NA	51	coding (776/804 nt)	ydjO	protein YdjO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (24/27);  total (24/27)

TAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGA  >  NC_000913/1812223‑1812489                                                                                                                                       |                                                                                                                                     tAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAgggccg                                                                                                                                 <  1:1420170/140‑4 (MQ=255) tAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAgggccg                                                                                                                                 >  1:132729/1‑137 (MQ=255) tAGTGGCTCGATGACAGCTGGCACGCCGACCAGGCTGAGGCTGAAGCAAACCGATAAAGCCTTTTTGGAAAGCGCAGTCGACGCCCAACTGGCACCACATTGATTCTTTTTCTGGCAACCGTCCGATTCCTATAgggccg                                                                                                                                 >  2:1158829/1‑137 (MQ=255)      gCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGcc                                                                                                                           >  1:1237674/1‑140 (MQ=255)       cTCGATGACAGCTGGCACGCTGACCAGCCGGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCt                                                                                                                          <  1:532547/140‑1 (MQ=255)       cTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCt                                                                                                                          <  2:946719/140‑1 (MQ=255)                      cACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGtt                                                                                                           >  1:307157/1‑140 (MQ=255)                          cTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCAt                                                                                                       <  1:1352610/140‑1 (MQ=255)                             aCCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATcgc                                                                                                    >  1:1125900/1‑140 (MQ=255)                              ccagccagTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATcgcg                                                                                                   <  1:1682285/140‑1 (MQ=255)                                    agTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATg                                                                                             <  1:842346/140‑1 (MQ=255)                                       ggCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCgcggc                                                                                                 >  2:1626613/1‑133 (MQ=255)                                       ggCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCgcggc                                                                                                 <  1:1626613/133‑1 (MQ=255)                                        gCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCgcggc                                                                                                 >  2:627741/1‑132 (MQ=255)                                        gCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCgcggc                                                                                                 <  1:627741/132‑1 (MQ=255)                                          tGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACt                                                                                       <  1:154292/140‑1 (MQ=255)                                              gCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACt                                                                                   <  1:1219117/140‑1 (MQ=255)                                              gCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACt                                                                                   <  2:906162/140‑1 (MQ=255)                                                aaaCCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCa                                                                                 >  2:1507783/1‑140 (MQ=255)                                                aaaCCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCa                                                                                 <  2:857225/140‑1 (MQ=255)                                                   ccGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCatat                                                                              <  2:307157/140‑1 (MQ=255)                                                             cATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGAt                                                                    >  1:880426/1‑140 (MQ=255)                                                                    gATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGc                                                             >  2:1383803/1‑140 (MQ=255)                                                                         cGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGaaaa                                                        >  2:26694/1‑140 (MQ=255)                                                                         cGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGaaaa                                                        >  2:1024608/1‑140 (MQ=255)                                                                         cGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATAGTTAAACAGATCGTCAGCGaaaa                                                        >  1:1199216/1‑140 (MQ=255)                                                                         cGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTAAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGaaaa                                                        >  1:1050623/1‑140 (MQ=255)                                                                                  cgCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCa                                               >  2:1370993/1‑140 (MQ=255)                                                                                   gCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCaa                                              <  1:1507783/140‑1 (MQ=255)                                                                                   gCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCaa                                              >  1:888045/1‑140 (MQ=255)                                                                                   gCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCaa                                              <  2:1674024/140‑1 (MQ=255)                                                                                       aaCTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTa                                          >  1:1639770/1‑140 (MQ=255)                                                                                        aCTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTAt                                         <  2:888045/140‑1 (MQ=255)                                                                                             cacaCCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATa                                    <  1:26694/140‑1 (MQ=255)                                                                                             cacaCCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATa                                    <  2:1639770/140‑1 (MQ=255)                                                                                                  cATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTc                               <  2:880426/140‑1 (MQ=255)                                                                                                      aaTTCTTATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTgg                           <  1:1024608/140‑1 (MQ=255)                                                                                                            tATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATa                                    <  2:62914/125‑1 (MQ=255)                                                                                                            tATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATa                                    >  1:62914/1‑125 (MQ=255)                                                                                                            tATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaat                     >  2:987572/1‑140 (MQ=255)                                                                                                            tATGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaat                     >  1:1671240/1‑140 (MQ=255)                                                                                                              tGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTc                               <  2:1108303/128‑1 (MQ=255)                                                                                                              tGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTc                               >  1:1108303/1‑128 (MQ=255)                                                                                                              tGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatga                   >  2:185255/1‑140 (MQ=255)                                                                                                              tGCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatga                   >  1:370195/1‑140 (MQ=255)                                                                                                               gCTGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTAAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatgaa                  <  2:1050623/140‑1 (MQ=255)                                                                                                                 tGGCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatgaatg                <  1:1002721/140‑1 (MQ=255)                                                                                                                   gCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCa                       >  2:163651/1‑131 (MQ=255)                                                                                                                   gCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCa                       <  1:163651/131‑1 (MQ=255)                                                                                                                   gCAACGGTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAAtgtg              >  2:796832/1‑140 (MQ=255)                                                                                                                         gTCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTa        <  2:132729/140‑1 (MQ=255)                                                                                                                          tCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatgaa                  <  1:701054/129‑1 (MQ=255)                                                                                                                          tCCGTTTTGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCaatgaa                  >  2:701054/1‑129 (MQ=255)                                                                                                                               tttGTATA‑GGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCgaga  <  1:796832/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TAGTGGCTCGATGACAGCTGGCACGCTGACCAGCCAGTGGCTGAAGCAAACCGATAAAGCCATTCTGGATAGCGAAGACGACGCCGAACTGGCACACCATTAATTCTTATGCTGGCAACGGTCCGTTTTGTATAGGGGCCGTTGCCTTACTTTTTAAATGTTCCATCGCGGCGATGCATACTGACTCATATTTAAACAGATCGTCAGCGAAAAAAATGTCCAACTTATCAATACATTCCTGGCTCAATGAATGTGCCTGTAGCGAGA  >  NC_000913/1812223‑1812489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑