Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,071	Δ1 bp	coding (189/1671 nt)	menD ←	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,068	0	C	.	100.0%	282.4 / NA	67	coding (192/1671 nt)	menD	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (33/34);  total (33/34)

GCGATCGGCGGTTAAGAGAATCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGA  >  NC_000913/2378939‑2379200                                                                                                                                   |                                                                                                                                     gcgATCGGCGGTTAAGAGAATCAGGTTTTTTCCCGGTTAACCC‑GCTTCAATCAGGGGCGGATAGAGATTTGCCACCGCCGGGCCGGGGGGCACAAACACCGCCACCCGCTGCTTGCTGGCTTTTGCCCGCCCC‑GCGcccg                                                                                                                           >  2:546084/1‑138 (MQ=255)  cgATCGGCGGTTAAGAGAATCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGa                                                                                                                          <  1:563264/140‑1 (MQ=255)        gcggTTAAGAGAATCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGcccc                                                                                                                    <  1:1311512/140‑1 (MQ=255)            ttAAGAGAATCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAAcc                                                                                                                <  1:144214/140‑1 (MQ=255)               agagAATCA‑GTTTTTTTCCCGTTAAACCGGCTTCAATCAGTGCCGGGTAGAGATTTTCCACCGCCGCGCCGGGGGGCACAATCACCGCCCCCCGCCGCTTGCTGACTTTTGCCCGCCCC‑GCGCCCGATGCCCCAAccccc                                                                                                             >  2:1515775/1‑138 (MQ=255)                 agaATCA‑TTTTTTCCCCGTTTACCCCGCCTTCAACCATTCCAGGAAAGAAATTGCCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGc                                                                                                           <  2:836092/140‑1 (MQ=255)                   aaTCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTc                                                                                                         >  1:767379/1‑140 (MQ=255)                     tCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCAt                                                                                                       >  2:1505309/1‑140 (MQ=255)                     tCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCAt                                                                                                       <  1:200231/140‑1 (MQ=255)                     tCA‑GTTTTTCCCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCAt                                                                                                       <  2:391231/140‑1 (MQ=255)                      cA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATc                                                                                                      >  1:314300/1‑140 (MQ=255)                           ttttCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGaaa                                                                                                  <  1:170938/140‑1 (MQ=255)                             ttCTCCGGTTACCCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATg                                                                                                <  2:1280066/140‑1 (MQ=255)                             ttCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATg                                                                                                >  1:1625/1‑140 (MQ=255)                              tctcCGGTTACCCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATgg                                                                                               <  2:1602127/140‑1 (MQ=255)                              tctcCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATgg                                                                                               >  2:631704/1‑140 (MQ=255)                               ctcCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATggg                                                                                              <  1:711898/140‑1 (MQ=255)                               ccccGGTTACCCCGGCTTCAATCAGTGCCGGATAGAGTTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATggg                                                                                              <  1:413228/138‑1 (MQ=255)                                tcCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGgt                                                                                             >  1:832752/1‑140 (MQ=255)                                  cGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGgtgt                                                                                           >  1:264799/1‑140 (MQ=255)                                    gTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGc                                                                                                           <  2:616814/122‑1 (MQ=255)                                    gTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGc                                                                                                           >  1:616814/1‑122 (MQ=255)                                    gTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTgtgg                                                                                         >  2:430361/1‑140 (MQ=255)                                    gTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTgtgg                                                                                         >  1:337079/1‑140 (MQ=255)                                       aaCCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGtga                                                                                      >  2:1207305/1‑140 (MQ=255)                                        aCCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGtgaa                                                                                     >  2:967083/1‑140 (MQ=255)                                             gCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTgaatgaat                                                                                >  1:716842/1‑140 (MQ=255)                                              cTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGaatgaatg                                                                               >  1:201696/1‑140 (MQ=255)                                                tCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCg                                                                             <  1:1003806/140‑1 (MQ=255)                                                  aaTCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGa                                                                           >  1:365077/1‑140 (MQ=255)                                                              aTAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTccgccgc                                                               >  1:1285941/1‑140 (MQ=255)                                                                  agaTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCt                                                           >  2:1082340/1‑140 (MQ=255)                                                                  agaTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCt                                                           <  2:1625/140‑1 (MQ=255)                                                                  agaTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCt                                                           >  2:1491490/1‑140 (MQ=255)                                                                  agaTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCt                                                           >  1:1642573/1‑140 (MQ=255)                                                                  agaTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCt                                                           >  1:256723/1‑140 (MQ=255)                                                                  agaTTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCAACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCt                                                           >  2:800982/1‑140 (MQ=255)                                                                     tttGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAAc                                                        <  2:716842/140‑1 (MQ=255)                                                                       tGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGt                                                      >  1:991604/1‑140 (MQ=255)                                                                       tGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGt                                                      >  1:169875/1‑140 (MQ=255)                                                                       tGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGt                                                      >  1:272662/1‑140 (MQ=255)                                                                       tGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGt                                                      >  2:472173/1‑140 (MQ=255)                                                                       tGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGt                                                      <  1:1207305/140‑1 (MQ=255)                                                                          cACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTaa                                                   <  2:905641/140‑1 (MQ=255)                                                                             cgccgTGCCGGAGGTCACAATCACCGCCACCGGCTGCTGGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAAc                                                  >  2:58533/1‑138 (MQ=255)                                                                             cgccgTGCCGGAGGTCACAATCACCGCCACCGGCTGCTGGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAAc                                                  <  1:58533/138‑1 (MQ=255)                                                                               ccgTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGgtg                                              <  1:1505309/140‑1 (MQ=255)                                                                                  tGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTa                                            >  2:851323/1‑139 (MQ=255)                                                                                  tGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTa                                            <  1:851323/139‑1 (MQ=255)                                                                                  tGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAg                                           >  1:918874/1‑140 (MQ=255)                                                                                      ggAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcg                                       <  2:256723/140‑1 (MQ=255)                                                                                      ggAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcg                                       <  2:991604/140‑1 (MQ=255)                                                                                      ggAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcg                                       <  2:365077/140‑1 (MQ=255)                                                                                      ggAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcg                                       <  2:169875/140‑1 (MQ=255)                                                                                      ggAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcg                                       <  1:421896/140‑1 (MQ=255)                                                                                       gAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAAcgc                                      <  2:1659737/140‑1 (MQ=255)                                                                                            cacaATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGcc                                 >  1:48926/1‑140 (MQ=255)                                                                                            cacaATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGcc                                 <  2:1285941/140‑1 (MQ=255)                                                                                                 tCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTgggg                            <  2:264799/140‑1 (MQ=255)                                                                                                 tCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTgggg                            <  1:1491490/140‑1 (MQ=255)                                                                                                 tCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTgggg                            >  1:832207/1‑140 (MQ=255)                                                                                                 tCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTgggg                            <  2:1522960/140‑1 (MQ=255)                                                                                                 tCACCGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTgggg                            <  2:337079/140‑1 (MQ=255)                                                                                                     cGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGAt                        >  2:1418786/1‑140 (MQ=255)                                                                                                     cGCCACCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGAt                        <  1:631704/140‑1 (MQ=255)                                                                                                         aCCGGCTGCTTGCTGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAg                    <  2:48926/140‑1 (MQ=255)                                                                                                                 cttgctGACTTTCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCt            >  2:1451654/1‑140 (MQ=255)                                                                                                                          tttCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTg   <  1:1451654/140‑1 (MQ=255)                                                                                                                           ttCGCCAG‑CCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGa  >  1:8267/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     GCGATCGGCGGTTAAGAGAATCA‑GTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGATTTGCCACCGCCGTGCCGGAGGTCACAATCACCGCCACCGGCTGCTTGCTGACTTTCGCCAGCCCCAGCGCCAGATGCCCCAACCCACGCTCATCGAAATGGGTGTGGTGAATGAATGCGGAATTCTCCGCCGCCGCTAACGTTAACGGTGTAGAACGCGAGCCTGGGGCGATACAGATGTGTCTGACGCCGTGA  >  NC_000913/2378939‑2379200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑