Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2071.G90.4% 260.0 / 19.1 83noncoding (80/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base G (33/42);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606068‑4606341
                                                                                                                                              |                                                                                                                                         
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑a                                                                                                                                            <  1:170997/140‑1 (MQ=255)
    aGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCACGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑ggg‑g                                                                                                                                     >  1:264402/1‑140 (MQ=255)
      aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑ggg‑ggg                                                                                                                                   <  2:551788/140‑1 (MQ=255)
       tAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑ggg‑gggg                                                                                                                                  <  2:1247785/140‑1 (MQ=255)
        aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGCTATCGCCTCGTTTGGAGC‑G‑A‑‑ggg‑ggggg                                                                                                                                 >  2:1222788/1‑140 (MQ=255)
             gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑ggg‑ggggg                                                                                                                                 <  1:1075703/135‑1 (MQ=255)
             gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑ggg‑ggggg                                                                                                                                 >  2:1075703/1‑135 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGa                                                                                                                          <  1:586086/140‑1 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGa                                                                                                                          <  2:450816/140‑1 (MQ=255)
                cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGaa                                                                                                                         <  1:943222/140‑1 (MQ=255)
                 agggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAAc                                                                                                                        >  1:1683793/3‑140 (MQ=255)
                  aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAAcc                                                                                                                       >  1:494471/1‑140 (MQ=255)
                   ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAAccc                                                                                                                      <  1:84386/140‑1 (MQ=255)
                      gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCa                                                                                                                   <  1:697126/140‑1 (MQ=255)
                       ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAA‑‑GGG‑GGGGG‑ACTTGAACCCCCAc                                                                                                                  <  2:1077071/140‑1 (MQ=255)
                       ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCAc                                                                                                                  <  1:381297/140‑1 (MQ=255)
                       ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCAc                                                                                                                  <  2:1683793/140‑1 (MQ=255)
                       ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCAc                                                                                                                  >  2:491637/1‑140 (MQ=255)
                       ggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCAc                                                                                                                  <  2:981574/140‑1 (MQ=255)
                        gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGGGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACg                                                                                                                 >  2:788183/1‑140 (MQ=255)
                         ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGt                                                                                                                >  1:1207347/1‑140 (MQ=255)
                             ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGt                                                                                                            >  2:1634879/1‑140 (MQ=255)
                             ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGAGTCTACCAATTCCGCCGCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGt                                                                                                            <  2:554612/140‑1 (MQ=255)
                               gAACCCCCACGTCCGTAAGGACACTAACA‑CTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAg                                                                                                         <  2:991470/140‑1 (MQ=37)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAg                                                                                                         >  1:464147/1‑140 (MQ=255)
                                 aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AGGGGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAg                                                                                                         <  1:1645097/140‑1 (MQ=255)
                                 aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCACGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGa                                                                                                        <  2:264402/140‑1 (MQ=255)
                                      cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                   <  1:1496874/140‑1 (MQ=255)
                                      cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                   <  1:491637/140‑1 (MQ=255)
                                       aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                  >  1:606937/1‑140 (MQ=255)
                                        cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 <  1:1032326/140‑1 (MQ=255)
                                        cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                 <  2:1276606/140‑1 (MQ=255)
                                          tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                               >  2:543366/1‑140 (MQ=255)
                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                             <  2:464147/140‑1 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                             <  2:621438/139‑1 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                             >  1:621438/1‑139 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                            >  2:1492189/1‑140 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                            <  2:606937/140‑1 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                            >  1:635113/1‑140 (MQ=255)
                                                    cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                     <  1:389283/140‑1 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTat                                                                                    >  1:1388125/1‑139 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                    <  2:635113/140‑1 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                    >  2:922830/1‑140 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                    <  2:494471/140‑1 (MQ=255)
                                                     acTAACACCAGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACCTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                    <  2:896578/140‑1 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAACCTAacg                                                                                  >  2:501283/1‑140 (MQ=255)
                                                         acacCTGAAGCTAGCGCGTCAACCAAATCCGGGACCTAAACACAGTCAACTTACTATGTATGAAATCAACTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                <  2:850078/140‑1 (MQ=18)
                                                               gAAGCTAGCGCGTCTATCAAATCCGCCACCTACGAACAGTCATCTTACCATTTTTGATATCGCATCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                          <  2:42771/140‑1 (MQ=21)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                          >  2:1268354/1‑140 (MQ=255)
                                                                 aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                        >  1:520096/1‑140 (MQ=255)
                                                                 aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGAGATCTTTGCGTTTGGTGC‑G‑G‑‑GGGCGGAGG‑CCCATCACCCCCACCCCCGGAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                        >  2:1096308/1‑140 (MQ=21)
                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:926771/1‑140 (MQ=255)
                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc                                                                   >  1:16571/1‑139 (MQ=255)
                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTAC‑G‑A‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                   >  2:1541528/1‑140 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  2:1207347/140‑1 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:1268354/140‑1 (MQ=255)
                                                                         cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                <  1:1222788/140‑1 (MQ=255)
                                                                                cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  1:1541528/140‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  1:922830/140‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                        <  1:788183/140‑1 (MQ=255)
                                                                                  aTTCCGCCGCCTACGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGG‑G‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                       <  2:945411/140‑1 (MQ=21)
                                                                                      cgccgcCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  1:518961/140‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  1:1634879/140‑1 (MQ=255)
                                                                                       gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AGGGGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                   <  1:1042758/140‑1 (MQ=255)
                                                                                       gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                  >  2:1013947/1‑140 (MQ=255)
                                                                                       gCCACCTTCACTCAGGCATCTTACTTTTTTGGAAATAGCCACGTTTGGTGC‑G‑A‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                 <  2:1669184/140‑1 (MQ=18)
                                                                                        gcACCTTCGCACAGTCATCTTACTTTTTATGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                 <  2:11055/139‑1 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGcc                                                 >  2:1323465/1‑139 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                 >  2:667748/1‑140 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAgcacg                                                 >  2:126294/1‑136 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑GGG‑GGGGGTACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                 >  1:562896/1‑140 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  1:1492189/140‑1 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  2:926771/140‑1 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  1:667748/140‑1 (MQ=255)
                                                                                              tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                    <  2:419332/131‑1 (MQ=255)
                                                                                              tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                    >  1:419332/1‑131 (MQ=255)
                                                                                              tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                                           >  1:424373/1‑140 (MQ=255)
                                                                                              tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat                                           >  2:629865/1‑140 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               >  2:1176617/1‑128 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                               <  1:1176617/128‑1 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                   >  1:1527691/1‑140 (MQ=255)
                                                                                                         tCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                                >  1:441941/1‑140 (MQ=255)
                                                                                                          cTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                               >  1:1646676/1‑140 (MQ=255)
                                                                                                            tACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                             <  1:1096308/140‑1 (MQ=255)
                                                                                                                  ttttGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                       >  2:1207407/1‑140 (MQ=255)
                                                                                                                    ttGATATCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                     >  2:1208753/1‑140 (MQ=255)
                                                                                                                        tatCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                 >  1:1506987/1‑140 (MQ=255)
                                                                                                                          tCGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg               <  2:441941/140‑1 (MQ=255)
                                                                                                                          acaacgagTTTGGTGCGG‑G‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg               <  2:695661/133‑1 (MQ=21)
                                                                                                                           cGCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg              <  2:1506987/140‑1 (MQ=255)
                                                                                                                            gCCTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggg             <  1:219599/140‑1 (MQ=255)
                                                                                                                             ccTCGTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggggg            >  2:526230/1‑139 (MQ=255)
                                                                                                                                 gTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg              <  2:407259/134‑1 (MQ=255)
                                                                                                                                 gTTTGGTGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg              >  1:407259/1‑134 (MQ=255)
                                                                                                                                   ttGGTGCGG‑G‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa      <  1:1056444/140‑1 (MQ=255)
                                                                                                                                       tGC‑G‑AG‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc  >  2:78555/1‑140 (MQ=255)
                                                                                                                                              |                                                                                                                                         
GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑G‑A‑‑GGG‑GGGGG‑ACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCC  >  NC_000913/4606068‑4606341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑