BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,338,055	T→C	D136G (GAC→GGC)	mlaD ←	intermembrane phospholipid transport system, substrate binding protein MlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,338,055	0	T	C	100.0%	508.4 / NA	138	D136G (GAC→GGC)	mlaD	intermembrane phospholipid transport system, substrate binding protein MlaD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (74/64); total (74/64)

Only 100 of 139 total aligned reads displayed.

TTGTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGT  >  NC_000913/3337918‑3338190

ttGTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGccc                                                                                                                                       >  1:614216/1‑140 (MQ=255)

   tACCCACAGGTTCAGTGGTTTCATTATTACC‑GGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGaa                                                                                                                                   <  2:141751/140‑1 (MQ=255)

          aGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTAt                                                                                                                             >  1:1580126/1‑140 (MQ=255)

             ttCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGc                                                                                                                          >  2:695554/1‑140 (MQ=255)

             ttCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGc                                                                                                                          >  1:1326544/1‑140 (MQ=255)

               cAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCa                                                                                                                        <  1:961289/140‑1 (MQ=255)

               cAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCa                                                                                                                        >  1:270531/1‑140 (MQ=255)

               cAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCa                                                                                                                        <  2:644226/140‑1 (MQ=255)

                aGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCa                                                                                                                        >  2:549993/1‑139 (MQ=255)

                 gTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATc                                                                                                                      <  1:695554/140‑1 (MQ=255)

                    gTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTAt                                                                                                                             <  2:1488457/130‑1 (MQ=255)

                      ttCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCtt                                                                                                                   >  2:990794/1‑138 (MQ=255)

                      ttCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCtt                                                                                                                   <  1:990794/138‑1 (MQ=255)

                      ttCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCa                                                                                                                 >  1:1198929/1‑140 (MQ=255)

                                ccTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGt                                                                                                       >  1:961708/1‑140 (MQ=255)

                                ccTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGt                                                                                                       >  1:209682/1‑140 (MQ=255)

                                   ggCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTccc                                                                                                    <  2:614216/140‑1 (MQ=255)

                                   ggCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTccc                                                                                                    >  2:879999/1‑140 (MQ=255)

                                   ggCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTccc                                                                                                    >  2:593592/1‑140 (MQ=255)

                                    gcgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTcccc                                                                                                   >  1:720047/1‑140 (MQ=255)

                                    gcgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTcccc                                                                                                   >  2:1418321/1‑140 (MQ=255)

                                     cgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTccc                                                                                                    >  1:343825/1‑138 (MQ=255)

                                     cgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTccc                                                                                                    <  2:343825/138‑1 (MQ=255)

                                     cgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCa                                                                                                  >  2:911568/1‑140 (MQ=255)

                                     cgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCa                                                                                                  >  2:1283590/1‑140 (MQ=255)

                                     cgcAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCa                                                                                                  >  2:333278/1‑140 (MQ=255)

                                        agcagcTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGtt                                                                                               <  2:961708/140‑1 (MQ=255)

                                          cagcTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTcc                                                                                             <  1:48740/140‑1 (MQ=255)

                                            gcTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCgg                                                                                           <  2:1129836/140‑1 (MQ=255)

                                                  gcATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTc                                                                                     <  1:755331/140‑1 (MQ=255)

                                                              ttCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGtt                                                                         <  1:688988/140‑1 (MQ=255)

                                                               tCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGttt                                                                        <  2:209682/140‑1 (MQ=255)

                                                                cTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTa                                                                       <  1:333278/140‑1 (MQ=255)

                                                                   aTTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATg                                                                    <  2:1527561/140‑1 (MQ=255)

                                                                     tGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGcc                                                                  <  1:765239/140‑1 (MQ=255)

                                                                      gTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTa                                                                       <  2:1111426/134‑1 (MQ=255)

                                                                      gTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTa                                                                       >  1:1111426/1‑134 (MQ=255)

                                                                      gTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCa                                                                 >  1:436347/1‑140 (MQ=255)

                                                                      gTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCa                                                                 >  2:1569143/1‑140 (MQ=255)

                                                                        cATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGa                                                               >  1:1237799/1‑140 (MQ=255)

                                                                         aTCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGat                                                              >  2:1370349/1‑140 (MQ=255)

                                                                         aTCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGat                                                              >  1:298223/1‑140 (MQ=255)

                                                                         aTCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGat                                                              >  2:679986/1‑140 (MQ=255)

                                                                             ccTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTg                                                          <  2:1237799/140‑1 (MQ=255)

                                                                              cTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGt                                                         >  2:949673/1‑140 (MQ=255)

                                                                                 tactacCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTcc                                                      >  1:121828/1‑140 (MQ=255)

                                                                                 tactacCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTcc                                                      >  1:1241513/1‑140 (MQ=255)

                                                                                        gTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCcagca                                               >  1:516513/1‑140 (MQ=255)

                                                                                        gTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCcagca                                               >  1:1120673/1‑140 (MQ=255)

                                                                                           aaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGc                                            <  1:1283590/140‑1 (MQ=255)

                                                                                           aaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGc                                            >  1:1538698/1‑140 (MQ=255)

                                                                                           aaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGc                                            >  2:1100577/1‑140 (MQ=255)

                                                                                           aaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGc                                            >  1:815192/1‑140 (MQ=255)

                                                                                           aaGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGc                                            >  1:573753/1‑140 (MQ=255)

                                                                                            aGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGcc                                           <  1:1569143/140‑1 (MQ=255)

                                                                                              gAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCcag                                                 <  2:235692/132‑1 (MQ=255)

                                                                                              gAACTGACCAATGAGATCTTCCAACACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCgg                                         <  2:698464/140‑1 (MQ=255)

                                                                                                aCTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGaa                                       >  2:29518/1‑140 (MQ=255)

                                                                                                 cTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCcag                                                 >  1:867609/1‑129 (MQ=255)

                                                                                                 cTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCcag                                                 <  2:867609/129‑1 (MQ=255)

                                                                                                  tGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGt                                     >  1:97927/1‑140 (MQ=255)

                                                                                                   gACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTa                                    >  2:1117580/1‑140 (MQ=255)

                                                                                                   gACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTa                                    >  2:363837/1‑140 (MQ=255)

                                                                                                   gACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTa                                    <  1:1053681/140‑1 (MQ=255)

                                                                                                        aTGAGATCTTCCAGCACCATCGTAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAAt                               >  2:427349/1‑140 (MQ=255)

                                                                                                        aTGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAAt                               <  1:923445/140‑1 (MQ=255)

                                                                                                        aTGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAAt                               <  1:569391/140‑1 (MQ=255)

                                                                                                         tGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATg                              >  2:936930/1‑140 (MQ=255)

                                                                                                         tGAGATCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATg                              >  1:258302/1‑140 (MQ=255)

                                                                                                            gaTCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTc                           <  2:121828/140‑1 (MQ=255)

                                                                                                             aTCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCa                          <  2:815192/140‑1 (MQ=255)

                                                                                                             aTCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCa                          <  2:11558/140‑1 (MQ=255)

                                                                                                             aTCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCa                          <  1:394869/140‑1 (MQ=255)

                                                                                                              tCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAg                         >  2:544552/1‑140 (MQ=255)

                                                                                                              tCTTCCAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAg                         <  1:1450735/140‑1 (MQ=255)

                                                                                                                  ccAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGaa                     <  1:483066/140‑1 (MQ=255)

                                                                                                                  ccAGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGaa                     <  1:181997/140‑1 (MQ=255)

                                                                                                                    aGCACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACt                   <  2:1241513/140‑1 (MQ=255)

                                                                                                                      cACCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTgg                 >  2:784315/1‑140 (MQ=255)

                                                                                                                       aCCATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGt                >  1:1200016/1‑140 (MQ=255)

                                                                                                                         cATCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTAt              <  2:436347/140‑1 (MQ=255)

                                                                                                                          aTCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATc             >  2:728817/1‑140 (MQ=255)

                                                                                                                           tCGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTAt              <  2:178335/138‑1 (MQ=255)

                                                                                                                            cacAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGCTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTg           >  2:282847/3‑140 (MQ=255)

                                                                                                                            cGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGaa                     <  1:618725/130‑1 (MQ=255)

                                                                                                                            cGCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTg           >  2:609412/1‑140 (MQ=255)

                                                                                                                             gCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTgg          <  1:1418321/140‑1 (MQ=255)

                                                                                                                             gCAGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTgg          <  2:97927/140‑1 (MQ=255)

                                                                                                                               aGACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGaa        <  2:1326544/140‑1 (MQ=255)

                                                                                                                                gACTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAt       <  1:879999/140‑1 (MQ=255)

                                                                                                                                 aCTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtg      <  1:949673/140‑1 (MQ=255)

                                                                                                                                  cTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtgt     <  2:806202/140‑1 (MQ=255)

                                                                                                                                  cTTAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtgt     <  2:470651/140‑1 (MQ=255)

                                                                                                                                   ttAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATTCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtgtg    >  1:1330756/1‑140 (MQ=255)

                                                                                                                                   ttAGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAAtgtg    >  1:239900/1‑140 (MQ=255)

                                                                                                                                     aGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGt                >  1:920517/1‑126 (MQ=255)

                                                                                                                                     aGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGt                <  2:920517/126‑1 (MQ=255)

                                                                                                                                     aGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGt  >  1:611747/1‑140 (MQ=255)

                                                                                                                                     aGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGt  >  2:1472634/1‑140 (MQ=255)

                                                                                                                                     aGTGCCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGt  >  2:268261/1‑140 (MQ=255)

TTGTACCCACAGGTTCAGTGGTTTCATTATTACCTGGCGCAGCAGCTGGCGCATCGCCACTATTCTTATTGTCATCGCCTTTACTACCGTAAAGGAACTGACCAATGAGATCTTCCAGCACCATCGCAGACTTAGTGTCCTGAATTGTATCGCCATCCTTCAGGATAGCAGTCCCCAGTTCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGT  >  NC_000913/3337918‑3338190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`