Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,519,791	C→T	E305K (GAG→AAG)	hofQ ←	DNA utilization protein HofQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,519,791	0	C	T	100.0%	267.6 / NA	76	E305K (GAG→AAG)	hofQ	DNA utilization protein HofQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/37);  total (39/37)

GACCTGCGTTTCGATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTCCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACTGGCGAT  >  NC_000913/3519654‑3519926                                                                                                                                          |                                                                                                                                        gACCTGCGTTTCGATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCa                                                                                                                                       >  2:174587/1‑140 (MQ=255)          ttCGATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGa                                                                                                                              <  1:72239/140‑1 (MQ=255)           tCGATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGAc                                                                                                                             >  2:403080/1‑140 (MQ=255)           tCGATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGAc                                                                                                                             >  2:1394322/1‑140 (MQ=255)             gATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACgg                                                                                                                           <  2:1126505/140‑1 (MQ=255)               tctcCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGcc                                                                                                                         <  1:174587/140‑1 (MQ=255)                    tGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGcc                                                                                                                         >  2:631216/1‑135 (MQ=255)                    tGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGcc                                                                                                                         <  1:631216/135‑1 (MQ=255)                      cTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTaa                                                                                                                  >  2:552826/1‑140 (MQ=255)                      cTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTaa                                                                                                                  >  2:433521/1‑140 (MQ=255)                      cTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTaa                                                                                                                  >  2:421633/1‑140 (MQ=255)                              tCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCAcc                                                                                                          <  2:141832/140‑1 (MQ=255)                                 ccAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGAc                                                                                                       >  2:1096724/1‑140 (MQ=255)                                  cAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACg                                                                                                      <  2:1252371/140‑1 (MQ=255)                                   aGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGt                                                                                                     >  1:1096139/1‑140 (MQ=255)                                    gCACTTCGCCATCGGCCTGCTATAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTc                                                                                                    >  2:1205492/1‑140 (MQ=255)                                     cACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTcg                                                                                                   >  1:1649234/1‑140 (MQ=255)                                          cGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCa                                                                                              >  2:778255/1‑140 (MQ=255)                                          cGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCa                                                                                              <  1:1178885/140‑1 (MQ=255)                                               tCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTc                                                                                         >  2:489541/1‑140 (MQ=255)                                               tCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTc                                                                                         >  1:1288318/1‑140 (MQ=255)                                                cGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTcc                                                                                        >  1:1064640/1‑140 (MQ=255)                                                 ggCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCCCTTTccc                                                                                       <  2:1645679/140‑1 (MQ=255)                                                 ggCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTccc                                                                                       <  1:124710/140‑1 (MQ=255)                                                   ccTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCg                                                                                     <  1:421633/140‑1 (MQ=255)                                                           aGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAAc                                                                             <  1:552826/140‑1 (MQ=255)                                                           aGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATATCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAAc                                                                             <  2:1650780/140‑1 (MQ=255)                                                            gCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCTCTTTCCCCGCTGGAAAcc                                                                            <  2:1288318/140‑1 (MQ=255)                                                            gCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAAcc                                                                            >  2:1476874/1‑140 (MQ=255)                                                                 tGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCTACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGata                                                                       >  2:745667/1‑140 (MQ=255)                                                                 tGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGata                                                                       >  2:777750/1‑140 (MQ=255)                                                                 tGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGata                                                                       >  2:711750/1‑140 (MQ=255)                                                                 tGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGata                                                                       >  2:1387970/1‑140 (MQ=255)                                                                  gCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGatat                                                                      >  2:173444/1‑140 (MQ=255)                                                                  gCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGagat                                                                      >  2:1293897/1‑140 (MQ=255)                                                                   ccccGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATg                                                                     <  1:489541/140‑1 (MQ=255)                                                                      cGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGaa                                                                  <  1:1338813/140‑1 (MQ=255)                                                                        gAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAAtt                                                                >  2:288624/1‑140 (MQ=255)                                                                         aaCGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAAttt                                                               <  1:173444/140‑1 (MQ=255)                                                                           cGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCg                                                             <  1:1476874/140‑1 (MQ=255)                                                                           cGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGAGATGGAATTTCg                                                             <  1:1293897/140‑1 (MQ=255)                                                                             ttCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCt                                                           >  1:121343/1‑140 (MQ=255)                                                                             ttCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCt                                                           <  1:199178/140‑1 (MQ=255)                                                                             ttCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCt                                                           >  1:469465/1‑140 (MQ=255)                                                                                tGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTccc                                                        <  1:1118756/140‑1 (MQ=255)                                                                                tGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTccc                                                        <  1:711750/140‑1 (MQ=255)                                                                                tGGCTGATGTGTAATTCCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTccc                                                        <  1:1589774/140‑1 (MQ=255)                                                                                  gCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCt                                                      >  1:1408157/1‑140 (MQ=255)                                                                                     taTGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCTCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGtt                                                   <  1:1679359/139‑1 (MQ=255)                                                                                      aTGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGttt                                                  >  2:333253/1‑140 (MQ=255)                                                                                       tgtgTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTa                                                 <  2:66517/140‑1 (MQ=255)                                                                                       tgtgTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTa                                                 >  1:66517/1‑140 (MQ=255)                                                                                              tttCAGCCGGATGCGACCTTTTTGTAACGCCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTgg                                          <  2:1330788/140‑1 (MQ=255)                                                                                              tttCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTgg                                          <  1:777750/140‑1 (MQ=255)                                                                                               ttCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGcggca                                         >  2:1119956/1‑136 (MQ=255)                                                                                                tCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCa                                        >  1:1632108/1‑140 (MQ=255)                                                                                                 cAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAg                                       <  1:403080/140‑1 (MQ=255)                                                                                                 cAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAg                                       <  1:1394322/140‑1 (MQ=255)                                                                                                  aGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAgg                                      <  2:1632108/140‑1 (MQ=255)                                                                                                       gATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGaa                                 <  1:288624/140‑1 (MQ=255)                                                                                                       gATGCGACCTTTTTGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGaa                                 <  1:1387970/140‑1 (MQ=255)                                                                                                                tttttGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAgg                        <  1:333253/140‑1 (MQ=255)                                                                                                                  tttGTAACACCGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGcc                      >  1:728860/1‑140 (MQ=255)                                                                                                                            cGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCTACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGAcgcg            >  2:250520/1‑140 (MQ=255)                                                                                                                            cGTGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGAcgcg            >  1:988349/1‑140 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCcag                    <  2:653869/130‑1 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCcag                    >  1:653869/1‑130 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGa          >  1:949745/1‑140 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGa          >  2:684810/1‑140 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGa          >  1:1545007/1‑140 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGa          <  1:433521/140‑1 (MQ=255)                                                                                                                              tGGGCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGa          <  1:745667/140‑1 (MQ=255)                                                                                                                                ggCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACt        >  1:1230716/1‑140 (MQ=255)                                                                                                                                 gCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACTg       <  2:1404216/140‑1 (MQ=255)                                                                                                                                 gCGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACTg       <  1:1096724/140‑1 (MQ=255)                                                                                                                                  cGTGACCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACTgg      <  2:1394200/140‑1 (MQ=255)                                                                                                                                      aCCTTCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACTGGCGat  >  1:951540/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GACCTGCGTTTCGATCTCCTGCTTATCAATCGCCAGCACTTCGCCATCGGCCTGCTGTAGCACCTGCCCCGGAACGTTCTGGCTGATGTGTAATTTCAGCCGGATGCGACCTTTTTGTAACACCGTGGGCGTGACCTCCATCCCCAGGACGGCCTCTTTAAATTCCACCGACGTCGCGCCACTTTCCCCGCTGGAAACCTGATATGGAATTTCGCTCCCCTGTTTAATGCTGGCAGGCTGAAGATGTGAGGCCAGCAGACGCGGACTGGCGAT  >  NC_000913/3519654‑3519926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑