Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,530,265	A→G	K164K (AAA→AAG)	hslO →	molecular chaperone Hsp33

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,530,265	0	A	G	100.0%	329.8 / NA	91	K164K (AAA→AAG)	hslO	molecular chaperone Hsp33
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/47);  total (44/47)

CGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGT  >  NC_000913/3530129‑3530401                                                                                                                                         |                                                                                                                                         cGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCg                                                                                                                                       >  2:1608898/1‑140 (MQ=255) cGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCg                                                                                                                                       >  2:617706/1‑140 (MQ=255)   aGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCTACGTAGACGGCAAGCCGGc                                                                                                                                     >  2:33837/1‑140 (MQ=255)   aGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGc                                                                                                                                     >  2:753422/1‑140 (MQ=255)   aGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGc                                                                                                                                     >  2:568672/1‑140 (MQ=255)   aGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGc                                                                                                                                     >  2:489150/1‑140 (MQ=255)     cGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTg                                                                                                                                   <  1:127342/140‑1 (MQ=255)      gAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGc                                                                                                                                  <  1:568672/140‑1 (MQ=255)        aGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAg                                                                                                                                <  1:1569572/140‑1 (MQ=255)         ggCGAATGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGc                                                                                                                                  <  1:455842/137‑1 (MQ=255)         ggCGAATGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGc                                                                                                                                  >  2:455842/1‑137 (MQ=255)         ggCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAgg                                                                                                                               <  1:648058/140‑1 (MQ=255)         ggCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAgg                                                                                                                               <  1:353683/140‑1 (MQ=255)         ggCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTACGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAgg                                                                                                                               >  2:987407/1‑140 (MQ=255)           cGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCg                                                                                                                             <  1:75904/140‑1 (MQ=255)           cGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCg                                                                                                                             <  1:489150/140‑1 (MQ=255)            gAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCgg                                                                                                                            <  1:753422/140‑1 (MQ=255)             aaCGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGt                                                                                                                           <  1:445622/140‑1 (MQ=255)              aCGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTa                                                                                                                          >  2:774674/1‑140 (MQ=255)              aCGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTa                                                                                                                          <  1:904583/140‑1 (MQ=255)                gCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTAtg                                                                                                                        <  1:1343928/140‑1 (MQ=255)                    tCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTAtgttgt                                                                                                                    >  2:664131/1‑140 (MQ=255)                    tCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTAtgttgt                                                                                                                    <  2:271027/140‑1 (MQ=255)                     cAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATgttgtt                                                                                                                   <  2:848141/140‑1 (MQ=255)                      aGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGttgttg                                                                                                                  <  1:1608898/140‑1 (MQ=255)                      aGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGttgttg                                                                                                                  <  1:318545/140‑1 (MQ=255)                      aGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGttgttg                                                                                                                  <  2:1627119/140‑1 (MQ=255)                        ggCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATgttgtt                                                                                                                   <  1:1562150/137‑1 (MQ=255)                        ggCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATgttgtt                                                                                                                   >  2:1562150/1‑137 (MQ=255)                          cGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAgg                                                                                                              >  2:1138537/1‑140 (MQ=255)                           gTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATgttgtt                                                                                                                   >  2:844306/1‑134 (MQ=255)                           gTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATgttgtt                                                                                                                   <  1:844306/134‑1 (MQ=255)                            tAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTAtg                                                                                                                        >  2:1188244/1‑128 (MQ=255)                            tAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTa                                                                                                            >  2:668452/1‑140 (MQ=255)                            tAGTTGGTCTGGAACGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTAtg                                                                                                                        <  1:1188244/128‑1 (MQ=255)                               ttGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATg                                                                                                         <  1:379275/140‑1 (MQ=255)                                       gAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCa                                                                                                 <  1:617706/140‑1 (MQ=255)                                               tACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGccc                                                                                         <  1:774674/140‑1 (MQ=255)                                               tACCCTGGCGGCCTGCCTGGAAGATTACTTTACGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGccc                                                                                         <  1:987407/140‑1 (MQ=255)                                                 cccTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCcag                                                                                       >  2:72307/1‑140 (MQ=255)                                                    tGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAAGTAATGCCTGCGCAAAATGCCcagcag                                                                                    >  2:1571472/1‑140 (MQ=255)                                                     ggcggcCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAgg                                                                                   >  2:1260037/1‑140 (MQ=255)                                                      gcggcCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  >  2:948087/1‑140 (MQ=255)                                                      gcggcCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  >  2:71566/1‑140 (MQ=255)                                                      gcggcCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  >  2:598303/1‑140 (MQ=255)                                                      gcggcCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  >  2:1237115/1‑140 (MQ=255)                                                      gcggcCTGCCTGGAAGACTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  >  2:1573827/1‑140 (MQ=255)                                                       cggcCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGgac                                                                                 <  1:1237115/140‑1 (MQ=255)                                                         gcctgcctGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  >  2:911308/1‑137 (MQ=255)                                                         gcctgcctGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGga                                                                                  <  1:911308/137‑1 (MQ=255)                                                             gcctgGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGgacga                                                                               <  1:1619783/136‑1 (MQ=255)                                                             gcctgGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGgacga                                                                               >  2:1619783/1‑136 (MQ=255)                                                                       ttACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTgg                                                                 <  1:948087/140‑1 (MQ=255)                                                                        tACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGc                                                                <  1:668452/140‑1 (MQ=255)                                                                            ttATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACg                                                            <  1:1138537/140‑1 (MQ=255)                                                                             tATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGc                                                           >  2:1244844/1‑140 (MQ=255)                                                                               tGCGGTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACCTTGACCACCTGGCGACGCTa                                                         >  2:1472022/1‑140 (MQ=255)                                                                                gCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTaa                                                        >  2:835507/1‑140 (MQ=255)                                                                                gCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTaa                                                        >  2:1549935/1‑140 (MQ=255)                                                                                    tCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGc                                                                >  2:1488487/1‑128 (MQ=255)                                                                                    tCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGc                                                                <  1:1488487/128‑1 (MQ=255)                                                                                    tCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCCACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGa                                                    >  2:200974/1‑140 (MQ=255)                                                                                     cTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCAGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGaa                                                   >  2:1492395/1‑140 (MQ=255)                                                                                          cAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCAt                                              <  1:1508273/140‑1 (MQ=255)                                                                                          cAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCAt                                              <  1:703816/140‑1 (MQ=255)                                                                                             cTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCaa                                           <  1:664131/140‑1 (MQ=255)                                                                                             cTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCaa                                           >  2:158565/1‑140 (MQ=255)                                                                                             cTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCaa                                           <  1:640577/140‑1 (MQ=255)                                                                                             cTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCaa                                           <  1:71566/140‑1 (MQ=255)                                                                                             cTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCaa                                           >  2:1000286/1‑140 (MQ=255)                                                                                             cTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCaa                                           >  2:1460195/1‑140 (MQ=255)                                                                                                ccGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAAc                                        >  2:701458/1‑140 (MQ=255)                                                                                                ccGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAAc                                        >  2:627870/1‑140 (MQ=255)                                                                                                  gACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCg                                      >  2:343651/1‑140 (MQ=255)                                                                                                     gcgcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaag                                   <  1:158565/140‑1 (MQ=255)                                                                                                     gcgcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaag                                   <  1:343651/140‑1 (MQ=255)                                                                                                     gcgcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaag                                   <  1:359384/140‑1 (MQ=255)                                                                                                     gcgcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaag                                   >  2:118204/1‑140 (MQ=255)                                                                                                     gcgcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaag                                   <  1:835507/140‑1 (MQ=255)                                                                                                       gcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaagaa                                 <  1:1573827/140‑1 (MQ=255)                                                                                                       gcCTGTTTATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCgaagaa                                 >  2:1518684/1‑140 (MQ=255)                                                                                                            tttATTCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAActgct                            <  1:1549935/140‑1 (MQ=255)                                                                                                                 tCGCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCt                       >  2:672323/1‑140 (MQ=255)                                                                                                                   gCACCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAAGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTa                     <  1:1571472/140‑1 (MQ=255)                                                                                                                     aCCGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTAcc                   >  2:582790/1‑140 (MQ=255)                                                                                                                      ccGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCg                  >  1:984247/1‑140 (MQ=255)                                                                                                                      ccGGCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCg                  >  1:1343024/1‑140 (MQ=255)                                                                                                                        ggCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGc                <  1:1244844/140‑1 (MQ=255)                                                                                                                         gCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCa               >  1:1278826/1‑140 (MQ=255)                                                                                                                         gCGACGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCa               >  2:1393534/1‑140 (MQ=255)                                                                                                                             cGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACg           <  1:1000286/140‑1 (MQ=255)                                                                                                                             cGTAGACGGCAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACg           <  1:703146/140‑1 (MQ=255)                                                                                                                                      cAAGCCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGtgttgt  <  1:72307/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGATTACTTTATGCGTTCTGAACAGCTGCCGACGCGCCTGTTTATTCGCACCGGCGACGTAGACGGCAAACCGGCTGCAGGCGGTATGTTGTTGCAGGTAATGCCTGCGCAAAATGCCCAGCAGGACGACTTTGACCACCTGGCGACGCTAACCGAAACCATCAAAACCGAAGAACTGCTGACCTTACCGGCAAACGAAGTGTTGT  >  NC_000913/3530129‑3530401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑