BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,542,066	A→T	I554F (ATC→TTC)	feoB →	Fe(2(+)) transporter FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,542,066	0	A	T	100.0%	409.7 / NA	119	I554F (ATC→TTC)	feoB	Fe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (58/61); total (58/61)

Only 100 of 120 total aligned reads displayed.

GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGG  >  NC_000913/3541930‑3542201

gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCa                                                                                                                                      <  2:218523/140‑1 (MQ=255)

   aGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCAccc                                                                                                                                   <  2:989657/140‑1 (MQ=255)

     gCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCg                                                                                                                                 >  2:30758/1‑140 (MQ=255)

     gCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCg                                                                                                                                 <  1:1561106/140‑1 (MQ=255)

     gCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCg                                                                                                                                 <  2:722164/140‑1 (MQ=255)

     gCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCg                                                                                                                                 <  1:650010/140‑1 (MQ=255)

         cGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCt                                                                                                                             <  2:1110863/140‑1 (MQ=255)

           ttCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTc                                                                                                                           <  2:1235519/140‑1 (MQ=255)

             cTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCaa                                                                                                                         <  1:1255010/140‑1 (MQ=255)

                cGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGcc                                                                                                                      >  1:137342/1‑140 (MQ=255)

                cGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGcc                                                                                                                      >  1:1382566/1‑140 (MQ=255)

                       gTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGc                                                                                                               >  1:309305/1‑140 (MQ=255)

                       gTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGc                                                                                                               >  1:470815/1‑140 (MQ=255)

                        tAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCg                                                                                                              <  2:441253/140‑1 (MQ=255)

                        tAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCg                                                                                                              <  2:45122/140‑1 (MQ=255)

                          aaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTg                                                                                                            >  2:1675303/1‑140 (MQ=255)

                           aGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                                                           <  1:1340584/140‑1 (MQ=255)

                           aGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                                                           <  1:442584/140‑1 (MQ=255)

                               atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                                       >  1:1503491/1‑140 (MQ=255)

                               atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                                       >  1:1591977/1‑140 (MQ=255)

                               atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                                       >  1:962474/1‑140 (MQ=255)

                               atcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                                       <  2:791916/140‑1 (MQ=255)

                               atcatcatcGTCAGCATTTTCCTAAGCGCTTTCAACAGCTTCTAGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                                       >  2:723868/1‑140 (MQ=255)

                                tcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGaa                                                                                                      <  2:962474/140‑1 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGc                                                                                                               <  1:515647/130‑1 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGc                                                                                                               >  2:515647/1‑130 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGa                                                                                                       >  1:1347347/1‑138 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                                                                     >  2:126671/1‑140 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                                                                     >  1:241840/1‑140 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                                                                     <  1:343051/140‑1 (MQ=255)

                                 catcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGGGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                                                                     >  2:1267619/1‑140 (MQ=255)

                                    catcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATa                                                                                                  >  2:162609/1‑140 (MQ=255)

                                      tcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCg                                                                                                              >  2:215756/1‑126 (MQ=255)

                                      tcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCg                                                                                                              <  1:215756/126‑1 (MQ=255)

                                       cGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACt                                                                                               >  2:1385977/1‑140 (MQ=255)

                                       cGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACt                                                                                               >  2:595797/1‑140 (MQ=255)

                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTgg                                                                                             >  1:189901/1‑140 (MQ=255)

                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTgg                                                                                             <  2:1591977/140‑1 (MQ=255)

                                             cATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                                                                         >  2:1441001/1‑140 (MQ=255)

                                               ttttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcaggca                                                                                       >  2:1545280/1‑140 (MQ=255)

                                                   ccTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTGGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACgg                                                                                   >  2:62485/1‑140 (MQ=255)

                                                   ccTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACgg                                                                                   >  2:1450399/1‑140 (MQ=255)

                                                     tGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGtt                                                                                 <  2:1448144/140‑1 (MQ=255)

                                                       aGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTgg                                                                               >  1:1510535/1‑140 (MQ=255)

                                                       aGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTgg                                                                               >  2:622063/1‑140 (MQ=255)

                                                        gcgcTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCACGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              <  2:1510535/140‑1 (MQ=255)

                                                        gcgcTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              <  1:1450399/140‑1 (MQ=255)

                                                        gcgcTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              <  2:309305/140‑1 (MQ=255)

                                                        gcgcTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              <  2:137342/140‑1 (MQ=255)

                                                        gcgcTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              <  2:898511/140‑1 (MQ=255)

                                                           cTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                                                                           <  1:68586/140‑1 (MQ=255)

                                                                     cTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTg                                                                 >  2:1536703/1‑140 (MQ=255)

                                                                      ttCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                                                                >  2:643248/1‑140 (MQ=255)

                                                                       tctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                               <  2:1204812/140‑1 (MQ=255)

                                                                       tctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                               <  1:403549/140‑1 (MQ=255)

                                                                        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              >  2:407530/1‑124 (MQ=255)

                                                                        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                                                              <  1:407530/124‑1 (MQ=255)

                                                                        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                               <  2:1700343/139‑1 (MQ=255)

                                                                        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                               >  1:1700343/1‑139 (MQ=255)

                                                                        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                                                              >  1:1171077/1‑140 (MQ=255)

                                                                        ctcGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                                                              >  2:1536235/1‑140 (MQ=255)

                                                                               aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGcc                                                               >  1:740335/1‑132 (MQ=255)

                                                                               aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                                       <  1:1536703/140‑1 (MQ=255)

                                                                               aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                                       <  2:1567655/140‑1 (MQ=255)

                                                                               aGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa                                                       >  1:655710/1‑140 (MQ=255)

                                                                                 cGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaag                                                     <  2:65934/140‑1 (MQ=255)

                                                                                 cGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaag                                                     <  1:577825/140‑1 (MQ=255)

                                                                                   ggAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaa                                                   <  2:572718/140‑1 (MQ=255)

                                                                                    gAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag                                                  <  1:1114131/140‑1 (MQ=255)

                                                                                         tcgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTa                                             >  2:1536019/1‑140 (MQ=255)

                                                                                         tcgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTa                                             <  1:520742/140‑1 (MQ=255)

                                                                                          cgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                                            <  1:684181/140‑1 (MQ=255)

                                                                                          cgtcgATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAg                                            <  2:209793/140‑1 (MQ=255)

                                                                                               aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGt                                       <  1:643248/140‑1 (MQ=255)

                                                                                               aTAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGt                                       >  2:1391570/1‑140 (MQ=255)

                                                                                                 aagaTCAACGACTCGGCGCTGGCGTCCGTCCGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTAc                                     <  2:1038319/137‑1 (MQ=255)

                                                                                                   cATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGc                                   >  2:1049378/1‑140 (MQ=255)

                                                                                                      cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                <  1:1545280/140‑1 (MQ=255)

                                                                                                      cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                <  1:1515447/140‑1 (MQ=255)

                                                                                                      cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                <  1:1391570/140‑1 (MQ=255)

                                                                                                      cAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCa                                <  2:457709/140‑1 (MQ=255)

                                                                                                           aCTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAcc                           <  1:62485/140‑1 (MQ=255)

                                                                                                           aCTCGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACAcc                           >  2:917572/1‑140 (MQ=255)

                                                                                                             tCGGCGCTGGCGTCCGTCAGTCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCct                         >  2:1414166/1‑140 (MQ=255)

                                                                                                              cGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                              <  1:1116088/134‑1 (MQ=255)

                                                                                                              cGGCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                              >  2:1116088/1‑134 (MQ=255)

                                                                                                               ggCGCTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCctct                       >  1:1447124/1‑140 (MQ=255)

                                                                                                                 cgcTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTac                     >  1:505521/1‑140 (MQ=255)

                                                                                                                  gcTGGCGTCCGTCAGCCGGGTGTTCAGCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAACGAAGTGGTAGTGGGTACGCTCAACACCCTCTaca                    <  2:1503491/140‑1 (MQ=255)

                                                                                                                  gcTGGCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTaca                    <  1:126671/140‑1 (MQ=255)

                                                                                                                     ggCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCg                 >  1:795710/1‑140 (MQ=255)

                                                                                                                      gCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                              <  2:65497/126‑1 (MQ=255)

                                                                                                                      gCGTCCGTCAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                              >  1:65497/1‑126 (MQ=255)

                                                                                                                         tccgtcAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTTCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa             >  2:1462360/1‑140 (MQ=255)

                                                                                                                         tccgtcAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                              <  2:1594970/123‑1 (MQ=255)

                                                                                                                         tccgtcAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAac                              >  1:1594970/1‑123 (MQ=255)

                                                                                                                         tccgtcAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGa             <  1:1675303/140‑1 (MQ=255)

                                                                                                                           cgtcAGCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGaaa           >  2:1172763/1‑140 (MQ=255)

                                                                                                                                gCCGGGTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATAtt      >  1:905578/1‑140 (MQ=255)

                                                                                                                                    ggTGTTCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAgg  <  2:655710/140‑1 (MQ=255)

GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTAGTGGGTACGCTCAACACCCTCTACACCGCAGAAAATATTCAGG  >  NC_000913/3541930‑3542201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`