Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,670	C→A	T1160K (ACG→AAG)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,670	0	C	A	98.8%	236.8 / ‑3.0	84	T1160K (ACG→AAG)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (44/39);  total (44/40)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

CCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAAC  >  NC_000913/3622539‑3622806                                                                                                                                    |                                                                                                                                         ccgcTGGCGGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAgggg                                                                                                                                  <  2:329720/140‑1 (MQ=17)                         gACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATatg                                                                                                          <  2:387268/140‑1 (MQ=255)                         gACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATatg                                                                                                          <  1:1512686/140‑1 (MQ=255)                          aCCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATatga                                                                                                         >  2:842801/1‑140 (MQ=12)                                  gCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATggg                                                                                                 <  2:983897/140‑1 (MQ=255)                                    aGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGc                                                                                               <  1:842801/140‑1 (MQ=12)                                    aGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGc                                                                                               >  1:465662/1‑140 (MQ=12)                                     gATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGc                                                                                               >  2:395048/1‑139 (MQ=12)                                     gATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGc                                                                                               <  1:395048/139‑1 (MQ=12)                                     gATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCa                                                                                              <  2:1046462/140‑1 (MQ=12)                                      aTGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCaa                                                                                             <  1:1466178/140‑1 (MQ=12)                                       tGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAc                                                                                            <  1:1116877/140‑1 (MQ=12)                                       tGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAc                                                                                            <  1:834404/140‑1 (MQ=12)                                       tGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAc                                                                                            <  2:1060188/140‑1 (MQ=12)                                        gCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAAcc                                                                                           >  2:420335/1‑140 (MQ=9)                                         cAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACct                                                                                          <  1:1668011/140‑1 (MQ=2)                                                  aTGGACCCGGTGTACGCGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCtgaatga                                                                                 >  2:144347/1‑140 (MQ=255)                                                       cccGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAgaga                                                                            >  2:225126/1‑140 (MQ=12)                                                           gtgtACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAAccc                                                                        >  1:627110/1‑140 (MQ=12)                                                            tgtACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCg                                                                       <  1:1545465/140‑1 (MQ=16)                                                             gtACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGc                                                                      >  1:162217/1‑140 (MQ=12)                                                                cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                                   >  1:644984/1‑140 (MQ=12)                                                                cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATc                                                                   >  1:131344/1‑140 (MQ=12)                                                                   gCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGc                                                                <  2:162217/140‑1 (MQ=16)                                                                     cGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTg                                                              >  2:1184069/1‑140 (MQ=12)                                                                     cGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTg                                                              >  1:903622/1‑140 (MQ=12)                                                                     cGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTg                                                              >  2:261209/1‑140 (MQ=12)                                                                     cGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTg                                                              >  2:410468/1‑140 (MQ=12)                                                                      ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAaga                                                                              <  2:896653/123‑1 (MQ=255)                                                                      ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAaga                                                                              >  1:896653/1‑123 (MQ=12)                                                                      ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                             >  1:1224829/1‑140 (MQ=12)                                                                      ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                             >  1:1321296/1‑140 (MQ=12)                                                                      ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                             >  1:28360/1‑140 (MQ=12)                                                                      ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                                             >  2:505876/1‑140 (MQ=12)                                                                        cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCAt                                                                    <  2:1352538/131‑1 (MQ=16)                                                                        cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCAt                                                                    >  1:1352538/1‑131 (MQ=12)                                                                        cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                                                           >  1:65918/1‑140 (MQ=12)                                                                        cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                                                           >  2:129114/1‑140 (MQ=12)                                                                        cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                                                           >  2:1615659/1‑140 (MQ=12)                                                                        cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGACAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                                                           >  1:1497924/1‑140 (MQ=255)                                                                                         tACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGcc                                          >  2:1439696/1‑140 (MQ=255)                                                                                         tACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGcc                                          >  2:865502/1‑140 (MQ=255)                                                                                           ccACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgg                                        <  2:472365/140‑1 (MQ=255)                                                                                            cACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCggg                                       >  1:1227827/1‑140 (MQ=255)                                                                                             aCTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg                                      <  1:1593973/140‑1 (MQ=255)                                                                                             aCTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg                                      <  2:131344/140‑1 (MQ=255)                                                                                             aCTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg                                      <  2:627110/140‑1 (MQ=255)                                                                                             aCTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg                                      <  1:1184069/140‑1 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg                                      >  2:1560468/1‑137 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg                                      <  1:1560468/137‑1 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag                                   <  1:1438689/140‑1 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag                                   >  1:1488291/1‑140 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag                                   <  2:48936/140‑1 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag                                   >  1:1291013/1‑140 (MQ=255)                                                                                                gCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag                                   >  2:1086958/1‑140 (MQ=255)                                                                                                 cGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagc                                  <  2:1435391/140‑1 (MQ=255)                                                                                                  gACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca                                 <  1:410468/140‑1 (MQ=255)                                                                                                  gACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca                                 <  1:505876/140‑1 (MQ=255)                                                                                                   aCCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag                                >  2:894308/1‑140 (MQ=255)                                                                                                   aCCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag                                >  1:605486/1‑140 (MQ=255)                                                                                                      aTCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTat                             <  2:1224829/140‑1 (MQ=255)                                                                                                        cgcgGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca                                 >  2:457518/1‑134 (MQ=255)                                                                                                        cgcgGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca                                 <  1:457518/134‑1 (MQ=255)                                                                                                        cgcgGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTatga                           >  1:1545474/1‑140 (MQ=255)                                                                                                        cgcgGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGCatga                           >  2:603075/1‑140 (MQ=11)                                                                                                          cgGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTatgatg                         <  2:1545474/140‑1 (MQ=255)                                                                                                           ggCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTAtgatga                        <  1:1086958/140‑1 (MQ=255)                                                                                                           ggCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTAtgatga                        <  1:1235638/140‑1 (MQ=255)                                                                                                              cTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATgagga                     >  2:424639/1‑140 (MQ=255)                                                                                                              cTGCCGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATgagga                     <  1:1615659/140‑1 (MQ=255)                                                                                                                 ccGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTc                  >  1:178473/1‑140 (MQ=255)                                                                                                                 ccGCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTc                  >  2:1284736/1‑140 (MQ=255)                                                                                                                   gCTGGCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCg                <  2:465137/140‑1 (MQ=255)                                                                                                                      ggCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGcc             <  2:1031952/140‑1 (MQ=255)                                                                                                                      ggCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGcc             <  1:1439696/140‑1 (MQ=255)                                                                                                                      ggCCCTTATCAGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGcc             <  2:16706/140‑1 (MQ=255)                                                                                                                       gcccttatcaGCAAGGAAGGGGCAACAGCGTGGTGCGCGGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCt            >  2:1637746/7‑140 (MQ=255)                                                                                                                       gcccttatcaGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc              >  1:1141642/7‑138 (MQ=255)                                                                                                                       gcccttatcaGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGc              <  2:1141642/132‑1 (MQ=255)                                                                                                                            tatcaGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTAtt       >  2:219507/2‑140 (MQ=255)                                                                                                                                aGCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTataa   <  2:465662/140‑4 (MQ=255)                                                                                                                                 gCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGcc             <  1:885625/129‑1 (MQ=255)                                                                                                                                 gCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAAc  >  2:1324254/1‑140 (MQ=255)                                                                                                                                 gCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAAc  <  1:219507/140‑1 (MQ=255)                                                                                                                                 gCAAGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAAc  >  2:1126830/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                         CCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAAC  >  NC_000913/3622539‑3622806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑