Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,923	T→C	intergenic (‑179/‑358)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,923	0	T	C	100.0%	364.9 / NA	98	intergenic (‑179/‑358)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (58/40);  total (58/40)

GGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTG  >  NC_000913/3771786‑3772058                                                                                                                                          |                                                                                                                                        ggCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGt                                                                                                                                       >  2:1259752/1‑140 (MQ=255)     gCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCTCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGAt                                                                                                                                   <  2:1176548/140‑1 (MQ=255)               aGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGAt                                                                                                                                   <  2:891751/130‑1 (MQ=255)               aGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGAt                                                                                                                                   >  1:891751/1‑130 (MQ=255)                 tCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGtta                                                                                                                       >  2:1151585/1‑138 (MQ=255)                 tCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGa                                                                                                                       >  1:64648/1‑140 (MQ=255)                 tCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGa                                                                                                                       >  2:168914/1‑140 (MQ=255)                  cAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGaa                                                                                                                      <  1:527017/140‑1 (MQ=255)                  cAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGaa                                                                                                                      <  1:837905/140‑1 (MQ=255)                  cAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGaa                                                                                                                      <  1:1259752/140‑1 (MQ=255)                    ggCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACg                                                                                                                    >  2:1424323/1‑140 (MQ=255)                    ggCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACg                                                                                                                    >  2:1178092/1‑140 (MQ=255)                       tGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCg                                                                                                                 <  2:64648/140‑1 (MQ=255)                        gATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGt                                                                                                                >  2:1422583/1‑140 (MQ=255)                        gATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGt                                                                                                                <  2:1457221/140‑1 (MQ=255)                           atTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAg                                                                                                             <  1:658827/140‑1 (MQ=255)                             tttGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGaa                                                                                                           <  1:754384/140‑1 (MQ=255)                               tGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGaa                                                                                                                      <  2:602031/127‑1 (MQ=255)                               tGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGaa                                                                                                                      >  1:602031/1‑127 (MQ=255)                                gACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAat                                                                                                        <  2:1060586/140‑1 (MQ=255)                                 aCAATAATCCGGGTTCGCGATTCTTGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAata                                                                                                       <  2:622117/140‑1 (MQ=255)                                 aCAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAata                                                                                                       <  2:964564/140‑1 (MQ=255)                                   aataatCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATc                                                                                                     >  1:1119864/1‑140 (MQ=255)                                   aataatCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATc                                                                                                     <  1:1158310/140‑1 (MQ=255)                                     taatCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCtt                                                                                                   >  2:1387443/1‑140 (MQ=255)                                     taatCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCtt                                                                                                   >  1:1339027/1‑140 (MQ=255)                                     taatCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCtt                                                                                                   >  2:1505482/1‑140 (MQ=255)                                     taatCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCtt                                                                                                   >  2:366574/1‑140 (MQ=255)                                        tCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTtgtg                                                                                                >  2:472961/1‑140 (MQ=255)                                          cGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAt                                                                                              <  1:958204/140‑1 (MQ=255)                                           gggTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAtt                                                                                             <  1:1387443/140‑1 (MQ=255)                                           gggTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAtt                                                                                             <  2:1339027/140‑1 (MQ=255)                                           gggTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAtt                                                                                             <  1:1505482/140‑1 (MQ=255)                                           gggTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAtt                                                                                             <  1:518279/140‑1 (MQ=255)                                           gggTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAtt                                                                                             <  2:1279736/140‑1 (MQ=255)                                            ggTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTc                                                                                            >  2:302334/1‑140 (MQ=255)                                             gTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCa                                                                                           >  2:1384625/1‑140 (MQ=255)                                               tCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGa                                                                                         >  1:1431845/1‑140 (MQ=255)                                                cgcgATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGAt                                                                                        >  1:1327136/1‑140 (MQ=255)                                                cgcgATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGAt                                                                                        >  1:1343446/1‑140 (MQ=255)                                                   gATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATcac                                                                                     <  1:472961/140‑1 (MQ=255)                                                      tctcGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACaaa                                                                                  >  2:279164/1‑140 (MQ=255)                                                           ccATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTc                                                                             <  2:1691799/140‑1 (MQ=255)                                                                      aaGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATc                                                                  >  2:142342/1‑140 (MQ=255)                                                                      aaGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATc                                                                  >  2:816866/1‑140 (MQ=255)                                                                      aaGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATc                                                                  >  2:236691/1‑140 (MQ=255)                                                                      aaGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATc                                                                  >  1:1099136/1‑140 (MQ=255)                                                                        gAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATcaa                                                                <  2:1457650/140‑1 (MQ=255)                                                                           taatTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATcaaaac                                                             >  1:1375455/1‑140 (MQ=255)                                                                           taatTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATcaaaac                                                             >  2:1195745/1‑140 (MQ=255)                                                                                  tAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAAtg                                                      <  1:563234/140‑1 (MQ=255)                                                                                   agagGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAAtgt                                                     >  1:126608/1‑140 (MQ=255)                                                                                      ggTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGac                                                  <  2:1497021/140‑1 (MQ=255)                                                                                       gTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 <  2:874074/140‑1 (MQ=255)                                                                                       gTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 <  2:126608/140‑1 (MQ=255)                                                                                       gTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 <  1:516837/140‑1 (MQ=255)                                                                                       gTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 <  1:302334/140‑1 (MQ=255)                                                                                       gTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 <  1:235997/140‑1 (MQ=255)                                                                                         gatgaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACact                                               >  1:365990/1‑140 (MQ=255)                                                                                          atgaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACacta                                              >  2:1578023/1‑140 (MQ=255)                                                                                          atgaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACacta                                              >  2:552847/1‑140 (MQ=255)                                                                                          atgaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACacta                                              >  1:1525010/1‑140 (MQ=255)                                                                                          atgaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACacta                                              >  2:956849/1‑140 (MQ=255)                                                                                          atgaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACacta                                              <  1:1384625/140‑1 (MQ=255)                                                                                            gaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACactact                                            <  1:1151585/140‑1 (MQ=255)                                                                                            gaGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACactact                                            <  2:651000/140‑1 (MQ=255)                                                                                               ttGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTcac                                         >  2:1131682/1‑140 (MQ=255)                                                                                                tGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGa                                                   >  2:1321398/1‑129 (MQ=255)                                                                                                tGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGa                                                   <  1:1321398/129‑1 (MQ=255)                                                                                                 gCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACAt                                       >  2:914136/1‑140 (MQ=255)                                                                                                  cTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 >  2:840430/1‑129 (MQ=255)                                                                                                  cTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGaca                                                 <  1:840430/129‑1 (MQ=255)                                                                                                  cTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACAtt                                      >  1:1192148/1‑140 (MQ=255)                                                                                                    tAGTTACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTa                                    >  1:976950/1‑140 (MQ=255)                                                                                                        tACATAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATg                                >  1:757324/1‑140 (MQ=255)                                                                                                            tAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACAttt                                     <  2:226540/131‑1 (MQ=255)                                                                                                            tAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACAttt                                     >  1:226540/1‑131 (MQ=255)                                                                                                            tAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAt                            >  1:885896/1‑140 (MQ=255)                                                                                                            tAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAt                            >  1:1326729/1‑140 (MQ=255)                                                                                                            tAACGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAt                            >  2:506136/1‑140 (MQ=255)                                                                                                             aaCGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAtt                           >  2:460285/1‑140 (MQ=255)                                                                                                               cGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAtttt                         >  2:320951/1‑140 (MQ=255)                                                                                                               cGATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAtttt                         >  2:1621049/1‑140 (MQ=255)                                                                                                                gATTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAttttt                        >  1:1686565/1‑140 (MQ=255)                                                                                                                 aTTGTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTa                       >  2:1198076/1‑140 (MQ=255)                                                                                                                    gTATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCg                    <  2:1686565/140‑1 (MQ=255)                                                                                                                     tATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGa                   >  2:771495/1‑140 (MQ=255)                                                                                                                     tATGACGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGa                   >  1:1332423/1‑140 (MQ=255)                                                                                                                         aCGAAGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAAt               >  2:677845/1‑140 (MQ=255)                                                                                                                             aGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcg             >  2:272967/1‑138 (MQ=255)                                                                                                                             aGGCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcg             <  1:272967/138‑1 (MQ=255)                                                                                                                              ggCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcgccg          <  1:677845/140‑1 (MQ=255)                                                                                                                               gCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcgccgc         >  1:1175171/1‑140 (MQ=255)                                                                                                                               gCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcgccgc         >  1:1271342/1‑140 (MQ=255)                                                                                                                               gCATAACATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcgccgc         >  2:934032/1‑140 (MQ=255)                                                                                                                                   aaCATGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCttg     >  1:1036155/1‑140 (MQ=255)                                                                                                                                      aTGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCttgttg  <  2:1326729/140‑1 (MQ=255)                                                                                                                                      aTGCCGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCttgttg  >  1:1469905/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTG  >  NC_000913/3771786‑3772058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑