Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,481	A→G	T408A (ACC→GCC)	gpmM →	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,481	0	A	G	100.0%	338.2 / NA	94	T408A (ACC→GCC)	gpmM	2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/51);  total (43/51)

CACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGC  >  NC_000913/3786344‑3786615                                                                                                                                           |                                                                                                                                       cACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGcc                                                                                                                                      <  1:147763/140‑1 (MQ=255)  aCCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGGTAGGTCACGcc                                                                                                                                      >  2:907457/1‑140 (MQ=255)  aCCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCg                                                                                                                                     >  1:339818/1‑140 (MQ=255)  aCCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCg                                                                                                                                     >  2:1233031/1‑140 (MQ=255)  aCCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCg                                                                                                                                     <  2:604581/140‑1 (MQ=255)  aCCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCg                                                                                                                                     >  1:1503818/1‑140 (MQ=255)  aCCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCg                                                                                                                                     >  1:1589444/1‑140 (MQ=255)   ccGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCgg                                                                                                                                    <  1:257424/140‑1 (MQ=255)      aaaGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGt                                                                                                                                 <  1:303676/140‑1 (MQ=255)            gCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGa                                                                                                                           >  1:1656505/1‑140 (MQ=255)              tacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAg                                                                                                                         >  2:905953/1‑140 (MQ=255)                cctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAgcg                                                                                                                       <  1:1696056/140‑1 (MQ=255)                    cGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGcggcgg                                                                                                                   >  2:1011475/1‑140 (MQ=255)                    cGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGcggcgg                                                                                                                   >  2:321823/1‑140 (MQ=255)                      aTCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAGGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGtt                                                                                                                 >  2:52482/1‑140 (MQ=255)                      aTCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGtt                                                                                                                 >  2:181144/1‑140 (MQ=255)                         tGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTaaa                                                                                                              <  2:378516/140‑1 (MQ=255)                         tGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTaaa                                                                                                              <  1:166535/140‑1 (MQ=255)                         tGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTaaa                                                                                                              <  2:991761/140‑1 (MQ=255)                          gCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTaaaa                                                                                                             >  1:1367443/1‑139 (MQ=255)                          gCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGATAAAg                                                                                                             >  1:332124/1‑140 (MQ=255)                           cAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGc                                                                                                            >  2:779428/1‑140 (MQ=255)                              ccGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGt                                                                                                         >  1:496796/1‑140 (MQ=255)                                ggAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTaaaaagggtg                                                                                                       >  2:105487/1‑133 (MQ=255)                                ggAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTg                                                                                                       <  1:905953/140‑1 (MQ=255)                                 gAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGa                                                                                                      >  1:372424/1‑140 (MQ=255)                                   aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGTAAGCGGCGGTTAAAGTGGTTGAAg                                                                                                    <  2:1155583/140‑1 (MQ=255)                                   aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAg                                                                                                    <  1:1010798/140‑1 (MQ=255)                                   aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAg                                                                                                    <  1:1233031/140‑1 (MQ=255)                                     tGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAgcg                                                                                                  <  2:496796/140‑1 (MQ=255)                                     tGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAgcg                                                                                                  <  2:339818/140‑1 (MQ=255)                                     tGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGGAgcg                                                                                                  >  2:182287/1‑140 (MQ=255)                                       aGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCt                                                                                                <  2:372424/140‑1 (MQ=255)                                        gCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTg                                                                                               >  1:1080961/1‑140 (MQ=255)                                             gCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCa                                                                                          <  1:85953/140‑1 (MQ=255)                                              cAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTg                                                                                               <  1:196265/134‑1 (MQ=255)                                              cAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTg                                                                                               >  2:196265/1‑134 (MQ=255)                                                gagCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTg                                                                                       <  1:321823/140‑1 (MQ=255)                                                 agCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGc                                                                                      <  1:182287/140‑1 (MQ=255)                                                      aCCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAACGGGTGGAGCGGTGGGTTACTGCGTggg                                                                                 >  1:1067066/1‑139 (MQ=255)                                                      aCCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAACGGGTGAAGCGCTGGGGCACTGCggggg                                                                                 >  2:1178765/1‑139 (MQ=255)                                                      aCCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGGGCTGGGTTACCGCGTggg                                                                                 >  1:399052/1‑139 (MQ=255)                                                      aCCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAAAGGTTGAAGGGCCGGGTCACTGCggggg                                                                                 >  2:85953/1‑139 (MQ=255)                                                          aaaaaCTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGgaagaa                                                                             <  1:1564849/140‑1 (MQ=255)                                                                   ttGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGGAGCGCTGGATCACTGCGTGGGAGAAGTCGCGaaa                                                                    >  1:602970/1‑140 (MQ=255)                                                                    tGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAg                                                                   <  2:854638/140‑1 (MQ=255)                                                                     gCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAGCGGGTGGAGCGCTGGGGCACTGCGGGGGAGAAGGCGCGgaaag                                                                  >  2:208669/1‑138 (MQ=255)                                                                       ggCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCgg                                                                <  1:1503899/140‑1 (MQ=255)                                                                         ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGtt                                                              <  2:982823/140‑1 (MQ=255)                                                                         ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGtt                                                              <  1:1178765/140‑1 (MQ=255)                                                                         ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGtt                                                              <  1:1146128/140‑1 (MQ=255)                                                                         ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGtt                                                              <  2:866858/140‑1 (MQ=255)                                                                         ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGtt                                                              <  2:1251148/140‑1 (MQ=255)                                                                         ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGtt                                                              <  2:1324414/140‑1 (MQ=255)                                                                           aTCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGa                                                            <  1:105487/140‑1 (MQ=255)                                                                                 agCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCgt                                                      <  2:683306/140‑1 (MQ=255)                                                                                   cGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGTTGAAGCGCTGGGTCACTGCGTGGGAGAAGGCGCGAAAACGGGTGAGGCCgggg                                                    >  2:1468722/1‑140 (MQ=255)                                                                                    ggCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCgtggg                                                   <  2:159855/140‑1 (MQ=255)                                                                                     gCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAACGGGTTGAGCGCTGGGTCACCGCGGGGGAGAAGTCGCGGAAACGGGTGAGGCCgggggg                                                  >  2:563644/1‑139 (MQ=255)                                                                                         aTACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGAc                                              <  2:747504/140‑1 (MQ=255)                                                                                         aTACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGAc                                              <  2:1080961/140‑1 (MQ=255)                                                                                         aTACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGAc                                              <  2:1654706/140‑1 (MQ=255)                                                                                         aTACAACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGAc                                              <  1:616798/140‑1 (MQ=255)                                                                                              acacCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAAGGGGTGAAGAGCTGGGTCACTGCGTGGGAGAAGTCGCGAAAACGGGTGAGTCCGTGGGGGGGGAActg                                         >  2:683798/1‑140 (MQ=255)                                                                                                   atcatcTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGAt                                    <  1:52482/140‑1 (MQ=255)                                                                                                   atcatcTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGAt                                    <  1:181144/140‑1 (MQ=255)                                                                                                     catcTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCa                                  <  1:479293/140‑1 (MQ=255)                                                                                                     catcTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCa                                  <  1:563644/140‑1 (MQ=255)                                                                                                         tGTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGTTGAAGCGCTGGAACACTGCGTGGGAGAAGACACGAAAACGGGTGAGTCCCTGGGTGGGCAAATCCTGCTcccccc                              >  2:1239990/1‑138 (MQ=255)                                                                                                          gTAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCt                             <  1:683798/140‑1 (MQ=255)                                                                                                           tAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGGGCTGGGTCACTGCGGGGGAGAAAACGCGAAAACGGGTGAGTCCGTGGGGGGGCAACTGCTGGTCAcccccg                            >  2:739410/1‑136 (MQ=255)                                                                                                           tAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGGTCACTGCGTGGGAGAAATCGCGAAAGCGGGTGAGTCCGTGGGGGGGCAACTGCTGATCACCGccg                            >  2:1294717/1‑138 (MQ=255)                                                                                                           tAACTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGGTAAAAGGGGTGAAGGGGTGGGGCACTGCGGGGGAGGAGTCGCGGAAGAGGTTGAGTTCGTGGGGGGAGAAATGCTGATCACCGCTg                            >  2:452780/1‑140 (MQ=255)                                                                                                            aaCTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAAAGGGTGAAGCGCCGGGTCACTGCGGGGGAGAAAACGCGGAAAAGGGTGAGTGCGTGGGGGGGGAACTGCTGATCAcccccga                           >  2:1282407/1‑135 (MQ=255)                                                                                                             aCTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGAc                          <  1:80464/140‑1 (MQ=255)                                                                                                              cTATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAGCGGGTGAAGCGCTGGGTCACTGCGTGGGAGAAGTCGCGAAAGCGGGTGAGTCCGTGGGGGGGCAACTGCTGATCACCGCTGAcc                         >  2:1303421/1‑140 (MQ=255)                                                                                                               tATCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCa                        <  1:1282407/140‑1 (MQ=255)                                                                                                                aTCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTTAGGGGGTGAAGCGCTGGGTGACTGCGGGGGAGCAGACGCGCAACCGGCTCAGCCCGCGGGGGGGCAACTGCTGATCACCGCTGAcccc                       >  2:80464/1‑138 (MQ=255)                                                                                                                 tCCGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACg                      >  2:981763/1‑140 (MQ=255)                                                                                                                  ccGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACgg                     <  2:778385/140‑1 (MQ=255)                                                                                                                  ccGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACgg                     <  1:208669/140‑1 (MQ=255)                                                                                                                  ccGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGGTCACTGCGTGGAAGAAGTCGCGAAAGCGGGTGAGTCCGTGGGGGGGCAAATGCTGATCACCGCTGGCCACgg                     >  2:1328861/1‑140 (MQ=255)                                                                                                                   cGAACGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAACGGTTGGAGCGCTGGATCACTGCGTGGGAGAAGTCGCGAAAACGGGTGGGGCCGGGGGGGGGCAAATCCTGATCACCGCCGCCCAccgg                    >  2:1638837/1‑137 (MQ=255)                                                                                                                     aaCGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAACGGGTGAAGCGCTGGATCACTGCGTGGGAGAAGGCGCGAAAACGGTTGAGTCCGGGGGGGGGCAACTGCTGATCACCGCCGACCACGGTaa                  >  2:636345/1‑140 (MQ=255)                                                                                                                     aaCGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGGTCACTGCGTGGGAGAAGTCGCGAAAGCGGTTGAGGCCGTGGGGGGGCAACTGCTGATCACCCCCGACCACGGGaa                  >  2:140051/1‑140 (MQ=255)                                                                                                                       cGGCGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGTGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGAACAACTGCTGATCACCGCTGACCACGGTAACg                <  1:452780/140‑1 (MQ=255)                                                                                                                          cGACAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTg             <  1:1328861/140‑1 (MQ=255)                                                                                                                            aCAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAg           <  2:1067066/140‑1 (MQ=255)                                                                                                                            aCAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAg           <  1:739410/140‑1 (MQ=255)                                                                                                                            aCAT‑GGTAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAg           <  1:1294717/140‑1 (MQ=255)                                                                                                                                   tAGGTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATg     <  1:907457/140‑1 (MQ=255)                                                                                                                                    aGGTCACGCCGGGGTGATGGAAGCGGCGGTTTAAACGGGTGGAGGGCCGGGTCACTGCGTGGAAGAAATCGCGAAAGCGGGTGAGTCCGTGGGGGGGCAAATGCTGATCACCGCCGACCACGGGAACGCCGAGCAGATgc    >  2:1141755/1‑140 (MQ=255)                                                                                                                                     ggTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATgcg   <  1:779428/140‑1 (MQ=255)                                                                                                                                      gTCACGCCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGGAGAAGTCGCGAAAGCGGGTGAGTCCGTGGGGGGGCAACTGCTGGTCACCCCCGACCACGGTAACGCTGAGCAGATgcgc  >  2:721773/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                                       CACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACAT‑GGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGC  >  NC_000913/3786344‑3786615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑