Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,865	T→C	F10L (TTT→CTT)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,865	0	T	C	100.0%	293.4 / NA	80	F10L (TTT→CTT)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/33);  total (47/33)

CGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGC  >  NC_000913/3786728‑3786995                                                                                                                                          |                                                                                                                                   cgcCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGctt                                                                                                                                  >  1:1392802/1‑140 (MQ=255) cgcAGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGctt                                                                                                                                  >  2:468463/1‑140 (MQ=255)                 gCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCcatca                                                                                                                  >  1:1315792/1‑140 (MQ=255)                 gCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCcatca                                                                                                                  >  1:607662/1‑140 (MQ=255)                         gTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGcc                                                                                                          >  2:191495/1‑140 (MQ=255)                         gTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGcc                                                                                                          >  2:1664967/1‑140 (MQ=255)                          tATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCa                                                                                                         <  1:1282520/140‑1 (MQ=255)                             ggAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGccc                                                                                                                      <  1:1639257/124‑1 (MQ=255)                             ggAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGccc                                                                                                                      >  2:1639257/1‑124 (MQ=255)                                 aTCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCt                                                                                                  >  1:49800/1‑140 (MQ=255)                                  tCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGc                                                                                                           >  1:1639748/1‑130 (MQ=255)                                  tCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGc                                                                                                           <  2:1639748/130‑1 (MQ=255)                                   cccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTa                                                                                                >  2:1537173/1‑140 (MQ=255)                                    ccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAg                                                                                               >  2:1251490/1‑140 (MQ=255)                                    ccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAg                                                                                               <  2:1645902/140‑1 (MQ=255)                                    ccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAg                                                                                               >  1:804740/1‑140 (MQ=255)                                    ccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCACCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAg                                                                                               <  2:433622/140‑1 (MQ=255)                                     cGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgc                                                                                              >  2:832898/1‑140 (MQ=255)                                      gCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                             <  2:213570/140‑1 (MQ=255)                                      gCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                             >  1:1548771/1‑140 (MQ=255)                                      gCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                             >  1:1631128/1‑140 (MQ=255)                                      gCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                             <  1:1664967/140‑1 (MQ=255)                                      gCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                             <  1:191495/140‑1 (MQ=255)                                        aaGAGATGACTGGTAAGCCGCTTTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTT‑CAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTg                                                                                          <  2:1178927/140‑1 (MQ=255)                                         agagATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTg                                                                                          <  2:997068/140‑1 (MQ=255)                                         agagATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTg                                                                                          <  2:907026/140‑1 (MQ=255)                                                  cggTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACAATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTAttgt                                                                                 <  1:115711/139‑1 (MQ=255)                                                    gTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTAttgttg                                                                               >  1:1539962/1‑140 (MQ=255)                                                       aGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTgc                                                                            >  1:1519939/1‑140 (MQ=255)                                                       aGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTgc                                                                            >  1:1444327/1‑140 (MQ=255)                                                        gCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTgcg                                                                           <  2:707992/140‑1 (MQ=255)                                                         ccGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTgcgc                                                                          >  2:732663/1‑140 (MQ=255)                                                          cGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGcc                                                                         >  1:574108/1‑140 (MQ=255)                                                           gCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCt                                                                        >  1:1446330/1‑140 (MQ=255)                                                            cTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCtt                                                                       >  1:409947/1‑140 (MQ=255)                                                                    cGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATTACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGccc                                                               >  1:733708/1‑140 (MQ=255)                                                                            aatCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGAt                                                       <  2:891082/140‑1 (MQ=255)                                                                              tccctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGa                                                     >  1:1443568/1‑140 (MQ=255)                                                                                cctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGa                                                        <  1:554358/135‑1 (MQ=255)                                                                                cctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGa                                                        >  2:554358/1‑135 (MQ=255)                                                                                cctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGc                                                   >  1:1481071/1‑140 (MQ=255)                                                                                cctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGc                                                   >  1:987360/1‑140 (MQ=255)                                                                                   ccccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGa                                                        >  2:838659/1‑132 (MQ=255)                                                                                   ccccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGa                                                        <  1:838659/132‑1 (MQ=255)                                                                                   ccccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTg                                                >  1:1190275/1‑140 (MQ=255)                                                                                   ccccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTg                                                <  1:1601314/140‑1 (MQ=255)                                                                                     ccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGAc                                              <  2:607662/140‑1 (MQ=255)                                                                                       aTGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCa                                            >  2:1484639/1‑140 (MQ=255)                                                                                              gAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACt                                         <  2:347058/136‑1 (MQ=255)                                                                                              gAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACt                                         >  1:347058/1‑136 (MQ=255)                                                                                              gAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCaaa                                     >  2:1329466/1‑140 (MQ=255)                                                                                              gAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCaaa                                     >  2:606140/1‑140 (MQ=255)                                                                                               aaaGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACt                                         >  2:709387/1‑135 (MQ=255)                                                                                               aaaGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACt                                         <  1:709387/135‑1 (MQ=255)                                                                                               aaaGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAAt                                    >  1:1089498/1‑140 (MQ=255)                                                                                                   gCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTAt                                <  2:1481071/140‑1 (MQ=255)                                                                                                    cGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTAtt                               <  2:1392802/140‑1 (MQ=255)                                                                                                    cGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTAtt                               <  2:1443568/140‑1 (MQ=255)                                                                                                    cGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTAtt                               <  1:1251490/140‑1 (MQ=255)                                                                                                      aTTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCa                             >  1:811870/1‑140 (MQ=255)                                                                                                      aTTAATACCATGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCa                             >  1:1355392/1‑140 (MQ=255)                                                                                                               aTGACACGGGCCGTGACACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACAt                    <  2:1519939/140‑1 (MQ=255)                                                                                                               aTGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACAt                    <  2:987360/140‑1 (MQ=255)                                                                                                               aTGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACAt                    <  2:49800/140‑1 (MQ=255)                                                                                                                tGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATc                   >  1:313296/1‑140 (MQ=255)                                                                                                                tGACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATc                   >  1:256485/1‑140 (MQ=255)                                                                                                                 gACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACAt                    <  1:36260/138‑1 (MQ=255)                                                                                                                 gACACGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACAt                    >  2:36260/1‑138 (MQ=255)                                                                                                                   cacGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgcc                >  1:388845/1‑140 (MQ=255)                                                                                                                   cacGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgcc                >  1:1614007/1‑140 (MQ=255)                                                                                                                     cGGGCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgccgc              >  2:1605392/1‑140 (MQ=255)                                                                                                                      gggCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgcc                <  1:1032719/137‑1 (MQ=255)                                                                                                                      gggCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgcc                >  2:1032719/1‑137 (MQ=255)                                                                                                                      gggCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCa             >  2:608617/1‑140 (MQ=255)                                                                                                                        gCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgccg               <  2:1396751/136‑1 (MQ=255)                                                                                                                        gCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcgccg               >  1:1396751/1‑136 (MQ=255)                                                                                                                        gCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCaaa           >  1:1668936/1‑140 (MQ=255)                                                                                                                        gCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCaaa           >  1:384147/1‑140 (MQ=255)                                                                                                                        gCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCaaa           >  2:118608/1‑140 (MQ=255)                                                                                                                        gCCGTGAAACCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGCGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCaaa           <  2:1539962/140‑1 (MQ=255)                                                                                                                                aCCGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAgcgcg   <  1:659184/140‑1 (MQ=255)                                                                                                                                 ccGCGCAGGCTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGCGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAgcgcgc  <  2:1203150/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   CGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGC  >  NC_000913/3786728‑3786995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑