BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,809,339	T→C	V267A (GTA→GCA)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,339	0	T	C	99.2%	459.7 / ‑5.0	131	V267A (GTA→GCA)	waaA	KDO transferase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (67/63); total (67/64)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 132 total aligned reads displayed.

GACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGT  >  NC_000913/3809205‑3809473

gACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCAcccc                                                                                                                                   <  1:485923/140‑1 (MQ=255)

gACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCAcccc                                                                                                                                   >  2:122007/1‑140 (MQ=255)

gACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCAcccc                                                                                                                                   >  2:485923/1‑140 (MQ=255)

      gcgcCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATc                                                                                                                             >  2:492686/1‑140 (MQ=255)

           gccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGaa                                                                                                                        <  2:603004/140‑1 (MQ=255)

           gccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGaa                                                                                                                        <  2:1411383/140‑1 (MQ=255)

                      ccacACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGa                                                                                                             <  1:1637573/140‑1 (MQ=255)

                       cacaCCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGAt                                                                                                            >  2:1609046/1‑140 (MQ=255)

                         cacCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGc                                                                                                          >  2:779569/1‑140 (MQ=255)

                          acCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCA‑CCCGTCATCCGGAACGCTTCCCGGATGCGa                                                                                                        >  2:318809/1‑140 (MQ=255)

                            cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGAt                                                                                                       >  1:155579/1‑140 (MQ=255)

                            cGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGAt                                                                                                       >  2:402106/1‑140 (MQ=255)

                              cccGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGAt                                                                                                            <  2:651950/133‑1 (MQ=255)

                                 ggTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAAcc                                                                                                  >  1:1634126/1‑140 (MQ=255)

                                    aTGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTg                                                                                               <  1:1159609/140‑1 (MQ=255)

                                            ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAg                                                                                       >  2:397208/1‑140 (MQ=255)

                                            ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAg                                                                                       >  1:921523/1‑140 (MQ=255)

                                            ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAg                                                                                       >  2:1480364/1‑140 (MQ=255)

                                             ctccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATCGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      <  2:251181/138‑1 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGTTGCGATTAACCTTGTCCGCCAgg                                                                                      >  2:563114/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  2:1497448/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  2:586919/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  1:1520831/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  1:189817/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  2:1247096/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      <  2:456792/140‑1 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  1:732909/1‑140 (MQ=255)

                                             caccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGACATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      <  2:1265480/140‑1 (MQ=255)

                                                 aGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTgg                                                                                  >  2:134474/1‑140 (MQ=255)

                                                 aGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCA‑CCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGa                                                                                 <  1:318809/140‑1 (MQ=255)

                                                  gCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGTTGCGATTAACCTTGTCCGCCAGGCTGGa                                                                                 <  1:563114/140‑1 (MQ=255)

                                                  gCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGa                                                                                 <  2:95647/140‑1 (MQ=255)

                                                  gCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGa                                                                                 <  2:921523/140‑1 (MQ=255)

                                                    aCTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACt                                                                               <  1:1497448/140‑1 (MQ=255)

                                                    aCTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACt                                                                               <  1:397208/140‑1 (MQ=255)

                                                      tCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTaa                                                                             <  1:1243060/140‑1 (MQ=255)

                                                      tCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTaa                                                                             >  1:1321646/1‑140 (MQ=255)

                                                         cGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCt                                                                          <  2:1656991/140‑1 (MQ=255)

                                                          gAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCta                                                                         >  2:1441983/1‑140 (MQ=255)

                                                           aaGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCtat                                                                        >  1:1127648/1‑140 (MQ=255)

                                                             ggCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      <  1:1615407/124‑1 (MQ=255)

                                                             ggCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAgg                                                                                      >  2:1615407/1‑124 (MQ=255)

                                                             ggCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGAc                                                                                <  1:855680/130‑1 (MQ=255)

                                                             ggCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGAc                                                                                >  2:855680/1‑130 (MQ=255)

                                                             ggCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTatat                                                                      >  1:1449516/1‑140 (MQ=255)

                                                              gCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATc                                                                     <  1:91477/140‑1 (MQ=255)

                                                                   gagagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                <  1:134474/140‑1 (MQ=255)

                                                                   gagagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                <  1:1609046/140‑1 (MQ=255)

                                                                   gagagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                <  2:64442/140‑1 (MQ=255)

                                                                   gagagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                <  2:1345427/140‑1 (MQ=255)

                                                                      agTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGctc                                                             >  1:161037/1‑140 (MQ=255)

                                                                        tgtgGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCtt                                                           >  2:1486331/1‑140 (MQ=255)

                                                                        tgtgGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACACCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                >  2:804096/1‑135 (MQ=255)

                                                                         gtggtgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                <  1:467932/134‑1 (MQ=255)

                                                                         gtggtgATCGCCACACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                >  2:1394965/1‑134 (MQ=255)

                                                                         gtggtgATCGCCACACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                <  1:1394965/134‑1 (MQ=255)

                                                                             tgATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAggg                                                      <  2:230625/140‑1 (MQ=255)

                                                                              gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAgggg                                                     <  1:1247096/140‑1 (MQ=255)

                                                                              gATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAgggg                                                     <  2:467486/140‑1 (MQ=255)

                                                                                tCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGaa                                                   <  1:779569/140‑1 (MQ=255)

                                                                                tCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGaa                                                   >  2:1577095/1‑140 (MQ=255)

                                                                                 cgccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAg                                                  >  2:214433/1‑140 (MQ=255)

                                                                                 cgccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAg                                                  <  1:926567/140‑1 (MQ=255)

                                                                                  gccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACg                                                                >  1:560390/1‑125 (MQ=255)

                                                                                   ccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTc                                                <  1:586919/140‑1 (MQ=255)

                                                                                     gcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTccc                                              <  1:402106/140‑1 (MQ=255)

                                                                                        caTCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTc                                           <  1:1577095/140‑1 (MQ=255)

                                                                                              gCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAg                                     <  2:161037/140‑1 (MQ=255)

                                                                                              gCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAg                                     >  1:1322795/1‑140 (MQ=255)

                                                                                              gCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAg                                     <  1:223012/140‑1 (MQ=255)

                                                                                                aTTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCa                                   <  2:281689/140‑1 (MQ=255)

                                                                                                 ttGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCAc                                  >  2:471761/1‑140 (MQ=255)

                                                                                                  tGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACg                                 <  2:683242/140‑1 (MQ=255)

                                                                                                   gTTACAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                >  1:1223791/1‑140 (MQ=255)

                                                                                                      aCAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAgg                             <  2:1668616/140‑1 (MQ=255)

                                                                                                      aCAGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAgg                             <  1:1486331/140‑1 (MQ=255)

                                                                                                        aGCAATTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGtt                           <  1:214433/140‑1 (MQ=255)

                                                                                                            aTTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTgg                       >  1:1057051/1‑140 (MQ=255)

                                                                                                            aTTCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTgg                       >  2:1256163/1‑140 (MQ=255)

                                                                                                             ttCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGt                      <  2:1322795/140‑1 (MQ=255)

                                                                                                              tCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGTGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTggat                     >  1:1410045/1‑138 (MQ=255)

                                                                                                              tCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                <  2:391426/129‑1 (MQ=255)

                                                                                                              tCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGc                                >  1:391426/1‑129 (MQ=255)

                                                                                                              tCCCGAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGtt                     >  2:206466/1‑140 (MQ=255)

                                                                                                                  gAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGt                      <  1:1664038/135‑1 (MQ=255)

                                                                                                                  gAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGt                      >  2:1664038/1‑135 (MQ=255)

                                                                                                                  gAATTTATTGCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCg                 <  2:1057051/140‑1 (MQ=255)

                                                                                                                        aTTGCTCATCCTGGCACCCCGTCATCCGTAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                    <  1:156659/131‑1 (MQ=255)

                                                                                                                        aTTGCTCATCCTGGCACCCCGTCATCCGTAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTg                    >  2:156659/1‑131 (MQ=255)

                                                                                                                           gCTCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATgg        <  2:912037/140‑1 (MQ=255)

                                                                                                                             tCATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGc      <  2:44013/140‑1 (MQ=255)

                                                                                                                              cATCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCg     >  1:877282/1‑140 (MQ=255)

                                                                                                                               aTCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGa    >  2:1610676/1‑140 (MQ=255)

                                                                                                                               aTCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGa    >  2:19898/1‑140 (MQ=255)

                                                                                                                               aTCCTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGa    <  1:492686/140‑1 (MQ=255)

                                                                                                                                 ccTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt  >  2:1266612/1‑140 (MQ=255)

                                                                                                                                 ccTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt  <  1:865256/140‑1 (MQ=255)

                                                                                                                                 ccTGGCACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt  >  1:763458/1‑140 (MQ=255)

                                                                                                                                 ccTGGCACCCCGTCATCCGGAACACTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt  >  2:1206839/1‑140 (MQ=255)

                                                                                                                                 ccTGGCACCCCGTCATCCGGAACACTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGt  <  1:1206839/140‑1 (MQ=255)

GACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGT  >  NC_000913/3809205‑3809473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`