BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,910,873	T→C	Y218C (TAT→TGT)	pstS ←	phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,910,873	0	T	C	100.0%	405.7 / NA	110	Y218C (TAT→TGT)	pstS	phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (57/53); total (57/53)

Only 100 of 111 total aligned reads displayed.

AGCGAAGGTTTTGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACATAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCAGGT  >  NC_000913/3910737‑3911008

aGCGAAGGTTTTGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAAc                                                                                                                                      >  1:685268/1‑140 (MQ=255)

aGCGAAGGTTTTGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAAc                                                                                                                                      >  2:648490/1‑140 (MQ=255)

aGCGAAGGTTTTGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAAc                                                                                                                                      >  2:136627/1‑140 (MQ=255)

    aaGGTTTTGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAAt                                                                                                                                  >  2:12657/1‑140 (MQ=255)

          ttGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCa                                                                                                                              <  2:828669/138‑1 (MQ=255)

          ttGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCa                                                                                                                              >  1:828669/1‑138 (MQ=255)

              tCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGc                                                                                                                        <  2:1274500/140‑1 (MQ=255)

                cAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCa                                                                                                                       <  2:285804/139‑1 (MQ=255)

                cAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCa                                                                                                                       >  1:285804/1‑139 (MQ=255)

                 aGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGa                                                                                                                     >  1:814571/1‑140 (MQ=255)

                 aGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGa                                                                                                                     >  2:1550122/1‑140 (MQ=255)

                  gTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGAc                                                                                                                    >  2:687611/1‑140 (MQ=255)

                  gTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGAc                                                                                                                    >  1:1422814/1‑140 (MQ=255)

                          cTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACg                                                                                                                   <  1:1568351/133‑1 (MQ=255)

                          cTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACg                                                                                                                   >  2:1568351/1‑133 (MQ=255)

                            tttGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTgaacgaa                                                                                                          >  1:1567672/1‑140 (MQ=255)

                            tttGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTgaacgaa                                                                                                          <  2:254824/140‑1 (MQ=255)

                            tttGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTgaacgaa                                                                                                          >  1:381864/1‑140 (MQ=255)

                            tttGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTgaacgaa                                                                                                          <  2:1567672/140‑1 (MQ=255)

                               gctgcATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAAcgc                                                                                                       >  1:509721/1‑140 (MQ=255)

                                   cATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACgcggcg                                                                                                   <  1:687611/140‑1 (MQ=255)

                                      tAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATa                                                                                                >  1:1175442/1‑140 (MQ=255)

                                        gCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATAcc                                                                                              >  2:77067/1‑140 (MQ=255)

                                          gAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATa                                                                                                >  1:1421202/1‑136 (MQ=255)

                                          gAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATa                                                                                                <  2:1421202/136‑1 (MQ=255)

                                           aaGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACcgtc                                                                                           <  1:1605253/140‑1 (MQ=255)

                                                ttcttcGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTAc                                                                                      <  2:524795/140‑1 (MQ=255)

                                                ttcttcGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTAc                                                                                      <  1:1224354/140‑1 (MQ=255)

                                                ttcttcGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTAc                                                                                      <  1:1258565/140‑1 (MQ=255)

                                                    tcggtcggACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCttt                                                                                  <  1:609181/140‑1 (MQ=255)

                                                     cggtcggACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTa                                                                                 <  2:814571/140‑1 (MQ=255)

                                                        tcggACTAACCGGTTTACCATCAGGGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGTATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCg                                                                              >  1:176293/1‑140 (MQ=255)

                                                        tcggACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCg                                                                              >  2:347367/1‑140 (MQ=255)

                                                        tcggACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCg                                                                              <  1:12657/140‑1 (MQ=255)

                                                                      ttACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCATACCGATCgg                                                                <  2:381864/140‑1 (MQ=255)

                                                                      ttACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCgg                                                                <  1:1550122/140‑1 (MQ=255)

                                                                      ttACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCgg                                                                <  2:509721/140‑1 (MQ=255)

                                                                       tACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGc                                                               <  1:77067/140‑1 (MQ=255)

                                                                       tACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGc                                                               <  1:770308/140‑1 (MQ=255)

                                                                       tACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGc                                                               <  2:539649/140‑1 (MQ=255)

                                                                       tACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGc                                                               <  2:685268/140‑1 (MQ=255)

                                                                          cATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCAt                                                            >  2:1255094/1‑140 (MQ=255)

                                                                             cAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCAtttt                                                         >  1:1337873/1‑140 (MQ=255)

                                                                              aGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTa                                                        <  2:1021721/140‑1 (MQ=255)

                                                                              aGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTa                                                        >  1:1183688/1‑140 (MQ=255)

                                                                              aGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTa                                                        >  1:792631/1‑140 (MQ=255)

                                                                               gCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCAtttt                                                         <  2:475248/138‑1 (MQ=255)

                                                                               gCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCAtttt                                                         >  1:475248/1‑138 (MQ=255)

                                                                               gCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTAc                                                       <  1:1403257/140‑1 (MQ=255)

                                                                                 ggAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACgg                                                     <  2:1625773/140‑1 (MQ=255)

                                                                                 ggAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCA‑TTTACGGt                                                    <  1:136627/140‑1 (MQ=255)

                                                                                  gagaTCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGt                                                    <  1:1255094/140‑1 (MQ=255)

                                                                                  gagaTCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGt                                                    <  1:347367/140‑1 (MQ=255)

                                                                                   agaTCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGGACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTa                                                   >  2:619312/1‑140 (MQ=255)

                                                                                   agaTCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGGACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTa                                                   <  1:619312/140‑1 (MQ=255)

                                                                                       cAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGc                                                               >  2:1529463/1‑124 (MQ=255)

                                                                                       cAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGc                                                               <  1:1529463/124‑1 (MQ=255)

                                                                                          tttGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCa                                                             <  2:856381/123‑1 (MQ=255)

                                                                                          tttGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCa                                                             >  1:856381/1‑123 (MQ=255)

                                                                                           ttGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGt                                           <  1:1322146/140‑1 (MQ=255)

                                                                                             ggTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTAc                                         >  1:1504270/1‑140 (MQ=255)

                                                                                              gtgtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTAcc                                        >  2:166618/1‑140 (MQ=255)

                                                                                              gtgtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTAcc                                        <  2:1454588/140‑1 (MQ=255)

                                                                                               tgtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCAtttt                                                         <  2:1269301/122‑1 (MQ=255)

                                                                                               tgtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCAtttt                                                         >  1:1269301/1‑122 (MQ=255)

                                                                                               tgtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCa                                       >  2:602581/1‑140 (MQ=255)

                                                                                                gtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCaa                                      <  1:624299/140‑1 (MQ=255)

                                                                                                gtACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCaa                                      <  1:648490/140‑1 (MQ=255)

                                                                                                   cGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGt                                                    >  2:1430447/1‑123 (MQ=255)

                                                                                                   cGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGt                                                    <  1:1430447/123‑1 (MQ=255)

                                                                                                   cGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCaa                                      <  1:917447/137‑1 (MQ=255)

                                                                                                    gCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACgtt                                  >  1:1688195/1‑140 (MQ=255)

                                                                                                    gCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACgtt                                  >  1:585123/1‑140 (MQ=255)

                                                                                                      cAGGTTGTACTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACgttgt                                >  2:1317986/1‑140 (MQ=255)

                                                                                                         gttgttCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGtttt                             >  2:273411/1‑140 (MQ=255)

                                                                                                         gttgttCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGtttt                             >  1:1625363/1‑140 (MQ=255)

                                                                                                           tgttCTGCTTCGCGTAAGCATATTCAACACGACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTc                           <  1:1222801/140‑1 (MQ=255)

                                                                                                           tgttCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTc                           <  2:1337873/140‑1 (MQ=255)

                                                                                                           tgttCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTc                           <  2:1183688/140‑1 (MQ=255)

                                                                                                             ttCTGCTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCa                         >  2:259988/1‑140 (MQ=255)

                                                                                                                  cTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACgttg                                 <  2:22105/127‑1 (MQ=255)

                                                                                                                  cTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACgttg                                 >  1:22105/1‑127 (MQ=255)

                                                                                                                  cTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCtt                    >  2:1486237/1‑140 (MQ=255)

                                                                                                                  cTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCtt                    >  2:1579036/1‑140 (MQ=255)

                                                                                                                  cTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCtt                    >  2:40039/1‑140 (MQ=255)

                                                                                                                  cTTCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCtt                    >  1:1507227/1‑140 (MQ=255)

                                                                                                                   ttCGCGTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTc                   >  2:542780/1‑140 (MQ=255)

                                                                                                                       cgTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTc               >  1:1011945/1‑140 (MQ=255)

                                                                                                                       cgTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTc               >  2:325648/1‑140 (MQ=255)

                                                                                                                       cgTAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTc               >  1:740282/1‑140 (MQ=255)

                                                                                                                         tAAGCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCAc             <  2:749559/140‑1 (MQ=255)

                                                                                                                            gCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACttt          >  2:727783/1‑140 (MQ=255)

                                                                                                                            gCATATTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACttt          >  1:1160397/1‑140 (MQ=255)

                                                                                                                              atatTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCg        <  1:145449/140‑1 (MQ=255)

                                                                                                                               tatTCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGc       <  1:1486237/140‑1 (MQ=255)

                                                                                                                                 ttCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCa     <  1:273411/140‑1 (MQ=255)

                                                                                                                                 ttCAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCa     <  2:1175442/140‑1 (MQ=255)

                                                                                                                                   cAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCAgg   <  1:640516/140‑1 (MQ=255)

                                                                                                                                   cAACACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCAgg   <  1:1317986/140‑1 (MQ=255)

                                                                                                                                    aaCACAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCAGGt  >  1:992085/1‑140 (MQ=255)

AGCGAAGGTTTTGCTCCAGTCTGCACCTTTTGCTGCATTAGCGAAGTTTTCTTCGGTCGGACTAACCGGTTTACCATCAGCGGAGATCAGTTTGGTGTACGCCAGGTTGTTCTGCTTCGCGTAAGCATATTCAACATAACCAATTGCACCCGGCAGACGCTGAACGAACGCGGCGATACCGTCGTTACCTTTACCGCCCAGACCGATCGGCCATTTTACGGTAGAGCCAGTACCAACGTTGTTTTTCCACTCTTCGTTCACTTTCGCCAGGT  >  NC_000913/3910737‑3911008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`