BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	489,576	A→T	D1014V (GAT→GTT)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	489,576	0	A	T	100.0%	366.1 / NA	103	D1014V (GAT→GTT)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (48/55); total (48/55)

Only 100 of 104 total aligned reads displayed.

CGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCG  >  NC_000913/489444‑489711

cGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGaa                                                                                                                                  <  1:1413841/140‑1 (MQ=255)

  aTTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGaaaa                                                                                                                                <  2:401175/140‑1 (MQ=255)

   ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGaaaaa                                                                                                                               <  2:484628/140‑1 (MQ=255)

   ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGaaaaa                                                                                                                               <  1:1681333/140‑1 (MQ=255)

    tACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAg                                                                                                                              <  1:785779/140‑1 (MQ=255)

        gATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCg                                                                                                                          <  1:884850/140‑1 (MQ=255)

        gATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCg                                                                                                                          <  1:682289/140‑1 (MQ=255)

          tttCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTa                                                                                                                        <  1:505400/140‑1 (MQ=255)

           ttCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGAGCGTaa                                                                                                                       >  1:1094583/1‑140 (MQ=255)

            tCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTaaa                                                                                                                      <  1:20514/140‑1 (MQ=255)

               aTCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAgtg                                                                                                                   <  1:260060/140‑1 (MQ=255)

                           tGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcg                                                                                                       >  1:977832/1‑140 (MQ=255)

                            gATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcgg                                                                                                      <  2:335925/140‑1 (MQ=255)

                             atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcggc                                                                                                     >  1:1182091/1‑140 (MQ=255)

                             atcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcggc                                                                                                     >  1:266786/1‑140 (MQ=255)

                              tcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGgcggcg                                                                                                    <  2:1182091/140‑1 (MQ=255)

                                  cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcggc                                                                                                     >  1:1123636/1‑135 (MQ=255)

                                  cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcggc                                                                                                     <  2:1123636/135‑1 (MQ=255)

                                  cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTg                                                                                                >  1:1394356/1‑140 (MQ=255)

                                  cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTg                                                                                                >  1:1191289/1‑140 (MQ=255)

                                  cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTg                                                                                                >  1:1107518/1‑140 (MQ=255)

                                   ccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                               <  2:977832/140‑1 (MQ=255)

                                   ccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                               <  1:1346355/140‑1 (MQ=255)

                                    cGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAg                                                                                              <  2:547043/140‑1 (MQ=255)

                                    cGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAg                                                                                              <  1:1126683/140‑1 (MQ=255)

                                    cGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTAGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAg                                                                                              <  2:822121/140‑1 (MQ=255)

                                      aaCAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCa                                                                                            <  1:1097415/140‑1 (MQ=255)

                                      aaCAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCa                                                                                            >  2:1530710/1‑140 (MQ=255)

                                        cAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAcc                                                                                          <  2:266786/140‑1 (MQ=255)

                                        cAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAcc                                                                                          <  2:1107518/140‑1 (MQ=255)

                                           aGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCaa                                                                                       <  2:51099/140‑1 (MQ=255)

                                             cGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAgg                                                                                     >  1:578741/1‑140 (MQ=255)

                                                ttGTTACCGAGCGTCTGATCATCTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTg                                                                                  <  2:1545453/140‑1 (MQ=255)

                                                 tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTg                                                                                  <  2:39682/139‑1 (MQ=255)

                                                 tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTg                                                                                  >  1:39682/1‑139 (MQ=255)

                                                    tACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGc                                                                              >  2:933760/1‑140 (MQ=255)

                                                     aCCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa                                                                             <  1:864073/140‑1 (MQ=255)

                                                             tCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCaa                                                                     <  2:922670/140‑1 (MQ=255)

                                                                        ggTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtc                                                          >  1:1354584/1‑140 (MQ=255)

                                                                        ggTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtc                                                          >  1:611313/1‑140 (MQ=255)

                                                                        ggTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtc                                                          >  1:1667963/1‑140 (MQ=255)

                                                                        ggTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtc                                                          >  2:1373737/1‑140 (MQ=255)

                                                                          tCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtctt                                                        <  1:294747/140‑1 (MQ=255)

                                                                          tCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtctt                                                        <  1:1447670/140‑1 (MQ=255)

                                                                           cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCa                                                                      <  2:1239706/125‑1 (MQ=255)

                                                                           cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCa                                                                      >  1:1239706/1‑125 (MQ=255)

                                                                           cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGtcttc                                                       >  2:496151/1‑140 (MQ=255)

                                                                                actgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTAc                                                  <  1:933760/140‑1 (MQ=255)

                                                                                   gacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGc                                               >  1:1561/1‑140 (MQ=255)

                                                                                    acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCa                                              >  1:1234259/1‑140 (MQ=255)

                                                                                      actactACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAtt                                            <  2:611313/140‑1 (MQ=255)

                                                                                       ctactaCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAttt                                           <  1:1003885/140‑1 (MQ=255)

                                                                                          ctacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGt                                        >  1:1482957/1‑140 (MQ=255)

                                                                                          ctacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTggg                                        >  2:1465672/1‑139 (MQ=255)

                                                                                           tacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTg                                       >  1:400488/1‑140 (MQ=255)

                                                                                             cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGcc                                     >  2:361563/1‑140 (MQ=255)

                                                                                              gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    <  2:1394356/140‑1 (MQ=255)

                                                                                              gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    <  2:1434428/140‑1 (MQ=255)

                                                                                              gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    <  2:1354584/140‑1 (MQ=255)

                                                                                              gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    <  2:263267/140‑1 (MQ=255)

                                                                                              gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    >  1:550239/1‑140 (MQ=255)

                                                                                               cgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                   <  1:1530710/140‑1 (MQ=255)

                                                                                               cgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                   <  2:578741/140‑1 (MQ=255)

                                                                                               cgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                   >  1:21289/1‑140 (MQ=255)

                                                                                               cgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                   <  2:1468638/140‑1 (MQ=255)

                                                                                                gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGc                                  >  1:529694/1‑140 (MQ=255)

                                                                                                gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGc                                  >  2:329084/1‑140 (MQ=255)

                                                                                                gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGc                                  >  1:1549107/1‑140 (MQ=255)

                                                                                                gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGc                                  >  2:1280745/1‑140 (MQ=255)

                                                                                                  cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCAc                                >  1:35508/1‑140 (MQ=255)

                                                                                                    ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGt                              >  1:1246525/1‑140 (MQ=255)

                                                                                                     gTGATCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                             <  2:126158/140‑1 (MQ=255)

                                                                                                        aTCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGa                          >  2:1014584/1‑140 (MQ=255)

                                                                                                         tCCGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGAt                         <  1:1465672/140‑1 (MQ=255)

                                                                                                           cGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    >  1:600924/1‑127 (MQ=255)

                                                                                                           cGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                    <  2:600924/127‑1 (MQ=255)

                                                                                                           cGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGt                              <  1:931772/133‑1 (MQ=255)

                                                                                                           cGTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGt                              >  2:931772/1‑133 (MQ=255)

                                                                                                            gTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGcc                                     <  2:1756/125‑1 (MQ=255)

                                                                                                            gTCTCGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGcc                                     >  1:1756/1‑125 (MQ=255)

                                                                                                             tctcGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                             <  1:763384/132‑1 (MQ=255)

                                                                                                             tctcGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATACCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                             >  2:763384/1‑132 (MQ=255)

                                                                                                               tcGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                   <  1:1014584/140‑1 (MQ=255)

                                                                                                                cGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                   <  1:1578752/139‑1 (MQ=255)

                                                                                                                cGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                   <  2:683372/139‑1 (MQ=255)

                                                                                                                cGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                   >  2:1578752/1‑139 (MQ=255)

                                                                                                                cGGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                   >  1:683372/1‑139 (MQ=255)

                                                                                                                 ggCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                 <  2:35508/140‑1 (MQ=255)

                                                                                                                   cGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGc               <  2:357082/140‑1 (MQ=255)

                                                                                                                       gCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGc               <  2:173250/136‑1 (MQ=255)

                                                                                                                       gCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGc               >  1:173250/1‑136 (MQ=255)

                                                                                                                       gCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCt           >  2:1226885/1‑140 (MQ=255)

                                                                                                                          tATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa        <  2:550239/140‑1 (MQ=255)

                                                                                                                          tATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa        <  1:1541380/140‑1 (MQ=255)

                                                                                                                          tATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa        <  1:1436466/140‑1 (MQ=255)

                                                                                                                            tGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtg      >  2:1590164/1‑140 (MQ=255)

                                                                                                                            tGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtg      >  1:851189/1‑140 (MQ=255)

                                                                                                                            tGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtg      >  2:787198/1‑140 (MQ=255)

                                                                                                                            tGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtg      >  2:1126932/1‑140 (MQ=255)

                                                                                                                                tCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCg  <  2:1177731/140‑1 (MQ=255)

CGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCG  >  NC_000913/489444‑489711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`