Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,517,071	A→G	V216A (GTG→GCG)	fecR ←	regulator for fec operon, periplasmic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,517,071	0	A	G	100.0%	331.2 / NA	90	V216A (GTG→GCG)	fecR	regulator for fec operon, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/41);  total (49/41)

AGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAG  >  NC_000913/4516936‑4517207                                                                                                                                        |                                                                                                                                         aGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGAt                                                                                                                                      <  1:957464/140‑1 (MQ=255) aGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGAt                                                                                                                                      >  2:56580/1‑140 (MQ=255)        cTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGtttt                                                                                                                               >  2:1237428/1‑140 (MQ=255)          aTCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTg                                                                                                                             <  2:1625506/140‑1 (MQ=255)            cacctcaccCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTgcg                                                                                                                           <  1:1313531/140‑1 (MQ=255)             acctcaccCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTgcgc                                                                                                                          >  2:1172592/1‑140 (MQ=255)                  accCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCggggg                                                                                                                     >  1:1441492/1‑140 (MQ=255)                  accCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCggggg                                                                                                                     >  2:447039/1‑140 (MQ=255)                  accCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCggggg                                                                                                                     >  2:92098/1‑140 (MQ=255)                   cccAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGc                                                                                                                    >  1:721423/1‑140 (MQ=255)                    ccAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCa                                                                                                                   <  2:721423/140‑1 (MQ=255)                    ccAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCa                                                                                                                   <  1:56580/140‑1 (MQ=255)                       gCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACt                                                                                                                 >  2:293775/1‑139 (MQ=255)                       gCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACt                                                                                                                 <  1:293775/139‑1 (MQ=255)                       gCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTg                                                                                                                >  1:1017783/1‑140 (MQ=255)                       gCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGACAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTg                                                                                                                >  2:1180283/1‑140 (MQ=255)                        cGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTgg                                                                                                               >  1:1007848/1‑140 (MQ=255)                        cGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTgg                                                                                                               >  1:1338799/1‑140 (MQ=255)                         ggTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGc                                                                                                              <  1:883010/140‑1 (MQ=255)                             tATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCgaga                                                                                                          <  1:1373189/140‑1 (MQ=255)                             tATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCgaga                                                                                                          <  2:1441492/140‑1 (MQ=255)                             tATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCgaga                                                                                                          <  1:1558270/140‑1 (MQ=255)                               tCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAg                                                                                                        <  1:226669/140‑1 (MQ=255)                                  cTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCAc                                                                                                     >  1:771865/1‑140 (MQ=255)                                    gAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACtt                                                                                                   >  1:1329441/1‑140 (MQ=255)                                       gCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCca                                                                                                <  2:233485/140‑1 (MQ=255)                                               tGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgc                                                                                        <  1:447039/140‑1 (MQ=255)                                                 tCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgttg                                                                                      >  1:517025/1‑140 (MQ=255)                                                 tCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgctg                                                                                      >  2:1091014/1‑140 (MQ=255)                                                  ccTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgctgc                                                                                     >  1:107395/1‑140 (MQ=255)                                                   cTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgctgct                                                                                    <  2:1017783/140‑1 (MQ=255)                                                     tCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgctgctgc                                                                                  >  1:1005054/1‑140 (MQ=255)                                                     tCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGtgctgctgc                                                                                  >  2:645438/1‑140 (MQ=255)                                                             cTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAg                                                                          >  1:1432387/1‑140 (MQ=255)                                                             cTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAg                                                                          >  1:173508/1‑140 (MQ=255)                                                                      tcGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGaa                                                                 <  2:1338799/140‑1 (MQ=255)                                                                       cGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGaaa                                                                >  1:643051/1‑140 (MQ=255)                                                                       cGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGaaa                                                                >  1:1672617/1‑140 (MQ=255)                                                                        gTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAAt                                                               >  2:62077/1‑140 (MQ=255)                                                                          cATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTa                                                             <  2:1432387/140‑1 (MQ=255)                                                                                  ggTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCctgctg                                                     >  1:1226531/1‑140 (MQ=255)                                                                                    tttCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGc                                                   <  2:527987/140‑1 (MQ=255)                                                                                      tCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCgg                                                 <  1:1172592/140‑1 (MQ=255)                                                                                           gcgcCAATCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGt                                            <  2:479881/140‑1 (MQ=255)                                                                                            cgcCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGc                                                   <  2:1371694/132‑1 (MQ=255)                                                                                            cgcCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGc                                                   >  1:1371694/1‑132 (MQ=255)                                                                                            cgcCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTa                                           >  1:699669/1‑140 (MQ=255)                                                                                                 aaCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTc                                      >  1:508009/1‑140 (MQ=255)                                                                                                 aaCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTc                                      >  2:636212/1‑140 (MQ=255)                                                                                                 aaCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTc                                      >  2:28404/1‑140 (MQ=255)                                                                                                 aaCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCAC‑GGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCt                                     <  1:1040175/140‑1 (MQ=255)                                                                                                  aCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCt                                     <  2:185734/140‑1 (MQ=255)                                                                                                  aCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCt                                     <  2:508009/140‑1 (MQ=255)                                                                                                  aCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCt                                     <  1:1180283/140‑1 (MQ=255)                                                                                                       gagGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTccc                                >  1:1531546/1‑140 (MQ=255)                                                                                                        agGCGCTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCt                               >  1:278681/1‑140 (MQ=255)                                                                                                           cgcTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTaaa                            >  2:66039/1‑140 (MQ=255)                                                                                                           cgcTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTaaa                            >  2:1590644/1‑140 (MQ=255)                                                                                                             cTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGc                          >  2:1035605/1‑140 (MQ=255)                                                                                                             cTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGc                          >  1:1349531/1‑140 (MQ=255)                                                                                                             cTGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGC‑GGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCa                         >  2:417049/1‑140 (MQ=255)                                                                                                              tGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTa                              >  2:1160181/1‑135 (MQ=255)                                                                                                              tGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTa                              <  1:1160181/135‑1 (MQ=255)                                                                                                              tGAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCa                         <  1:645438/140‑1 (MQ=255)                                                                                                               gAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAg                        <  1:28404/140‑1 (MQ=255)                                                                                                               gAACTGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAg                        <  2:1007848/140‑1 (MQ=255)                                                                                                                   tGCAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAg                    <  1:309135/140‑1 (MQ=255)                                                                                                                     cAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCt                  >  1:910676/1‑140 (MQ=255)                                                                                                                     cAGGCTTTCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCt                  >  2:1682401/1‑140 (MQ=255)                                                                                                                          tttCACCAGCGTTCGTGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGccc             >  2:1637735/1‑140 (MQ=255)                                                                                                                          tttCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGccc             >  1:1362435/1‑140 (MQ=255)                                                                                                                          tttCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGccc             >  1:240869/1‑140 (MQ=255)                                                                                                                            tCACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAACTCTGTCCCTAAAGCAGTGAGCTGGCCCTg           >  2:1368664/1‑140 (MQ=255)                                                                                                                             cACCAGCGTTCGTGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          <  1:922170/140‑1 (MQ=255)                                                                                                                             cACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          >  1:151788/1‑140 (MQ=255)                                                                                                                             cACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          <  2:465431/140‑1 (MQ=255)                                                                                                                             cACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          <  1:62077/140‑1 (MQ=255)                                                                                                                             cACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          <  1:92098/140‑1 (MQ=255)                                                                                                                             cACCAGCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAACTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          <  1:1368664/140‑1 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          >  2:324814/1‑135 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGa          <  1:324814/135‑1 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGt     <  2:1561715/140‑1 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGt     <  2:1493764/140‑1 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGt     <  2:278681/140‑1 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGt     >  1:1493764/1‑140 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGt     <  1:944361/140‑1 (MQ=255)                                                                                                                                  gCGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGACGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGt     >  2:143602/1‑140 (MQ=255)                                                                                                                                   cGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTc    >  1:563881/1‑140 (MQ=255)                                                                                                                                   cGTTCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTc    <  2:173508/140‑1 (MQ=255)                                                                                                                                     ttCGCGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTTAGCTGGCCCTGACGGGTCAg  <  1:1590644/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAG  >  NC_000913/4516936‑4517207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑