Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	791,748	C→T	G103D (GGC→GAC)	galE ←	UDP‑glucose 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	791,748	0	C	T	100.0%	186.0 / NA	53	G103D (GGC→GAC)	galE	UDP‑glucose 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/30);  total (23/30)

AGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATA  >  NC_000913/791612‑791882                                                                                                                                         |                                                                                                                                       aGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGt                                                                                                                                     >  2:801917/1‑140 (MQ=255)      tttGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACa                                                                                                                                <  1:801917/140‑1 (MQ=255)          cGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACAttgt                                                                                                                            <  2:253008/140‑1 (MQ=255)            gtgtGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACAttgttg                                                                                                                          <  1:72099/140‑1 (MQ=255)             tgtgCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATtgttgt                                                                                                                         >  2:652668/1‑140 (MQ=255)             tgtgCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATtgttgt                                                                                                                         <  1:900603/140‑1 (MQ=255)             tgtgCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATtgttgt                                                                                                                         >  1:857958/1‑140 (MQ=255)              gtgCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTc                                                                                                                        >  1:500102/1‑140 (MQ=255)               tgCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCg                                                                                                                       >  2:1285676/1‑140 (MQ=255)                    gTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAata                                                                                                                  >  1:604104/1‑140 (MQ=255)                         gAAGCTTTCAACGTAT‑GAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAAAAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAg                                                                                                            >  1:1059131/1‑140 (MQ=255)                           aGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGc                                                                                                           <  2:335443/140‑1 (MQ=255)                           aGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGc                                                                                                           <  2:959232/140‑1 (MQ=255)                                       aTGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTAcc                                                                                               >  1:542776/1‑140 (MQ=255)                                               ttGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGcc                                                                                       >  2:1630146/1‑140 (MQ=255)                                                tGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGccc                                                                                      >  2:1396882/1‑140 (MQ=255)                                                  ggCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCAc                                                                                    >  2:126001/1‑140 (MQ=255)                                                  ggCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCAc                                                                                    >  1:66964/1‑140 (MQ=255)                                                      gATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCt                                                                                >  1:231237/1‑140 (MQ=255)                                                        tCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCttt                                                                              <  2:1029812/140‑1 (MQ=255)                                                            cATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGc                                                                          >  1:1049531/1‑140 (MQ=255)                                                               aaaCGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCg                                                                       <  1:195462/140‑1 (MQ=255)                                                                      ggCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGt                                                                >  1:825990/1‑140 (MQ=255)                                                                       gCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTg                                                               <  1:504885/140‑1 (MQ=255)                                                                       gCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTg                                                               <  2:604104/140‑1 (MQ=255)                                                                          gaggagCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCg                                                                    >  2:812596/1‑132 (MQ=255)                                                                          gaggagCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCg                                                                    <  1:812596/132‑1 (MQ=255)                                                                           aggagCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATc                                                           <  1:1285676/140‑1 (MQ=255)                                                                           aggagCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATc                                                           <  1:240800/140‑1 (MQ=255)                                                                           aggagCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATc                                                           <  2:1666008/140‑1 (MQ=255)                                                                              agCTAAAAAAAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACg                                                        <  2:1059131/140‑1 (MQ=255)                                                                               gCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGcc                                                                         <  1:137378/122‑1 (MQ=255)                                                                               gCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGcc                                                                         >  2:137378/1‑122 (MQ=255)                                                                               gCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACgg                                                       <  1:1630146/140‑1 (MQ=255)                                                                                   aaaaTAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTc                                                   <  2:1343461/140‑1 (MQ=255)                                                                                             ttttGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGa                                         <  2:1565687/140‑1 (MQ=255)                                                                                             ttttGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGa                                         <  1:126001/140‑1 (MQ=255)                                                                                              tttGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGAt                                        <  1:1396882/140‑1 (MQ=255)                                                                                               ttGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCGTTCAACCCGGCGAAGTTGAACACGGTATCGATAGCGTGATc                                       >  1:1225085/1‑140 (MQ=255)                                                                                                  aCGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTg                                    <  2:857958/140‑1 (MQ=255)                                                                                                       aGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGa                               <  2:1621365/140‑1 (MQ=255)                                                                                                               gcATGGCGCTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGtc                       <  2:186295/140‑1 (MQ=255)                                                                                                                      gcTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACg                >  1:910657/1‑140 (MQ=255)                                                                                                                       cTAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGc               <  2:910657/140‑1 (MQ=255)                                                                                                                        tAATCAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCt              <  2:1049531/140‑1 (MQ=255)                                                                                                                            cAGGCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATGGTGCAGGATCTCGGTCATCAACGCTTCGt          <  2:1424582/140‑1 (MQ=255)                                                                                                                              ggCGCAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTa        <  1:652668/140‑1 (MQ=255)                                                                                                                               gcgcAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTAc       >  1:594556/1‑140 (MQ=255)                                                                                                                               gcgcAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTAc       >  1:1595740/1‑140 (MQ=255)                                                                                                                               gcgcAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGCGATCGTGCAGGATCTCGGTCATCAACGCt              <  1:993378/133‑1 (MQ=255)                                                                                                                               gcgcAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGCGATCGTGCAGGATCTCGGTCATCAACGCt              >  2:993378/1‑133 (MQ=255)                                                                                                                                  cAGAGTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGaa    >  1:888062/1‑140 (MQ=255)                                                                                                                                    gagTGTCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAata  <  2:888062/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AGGGCTTTGCGGTGTGCCGGTCGGGAAGCTTTCAACGTATGGAATTTTGGGCTGATCGCCATAAACGGTGGCGGAGGAGCTAAAAATAAAGTTTTTGACGTTAGCGGCGCGCATGGCGCTAATCAGGCGCAGAGTGCCGTTGACATTGTTGTCGTAATATTCCAGCGGTTTTTGTACCGATTCGCCCACGGCTTTCAGCCCGGCGAAGTGGATCACGGTGTCGATAGCGTGATCGTGCAGGATCTCGGTCATCAACGCTTCGTTACGAATA  >  NC_000913/791612‑791882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑