Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	831,857	A→G	Y329C (TAT→TGT)	rhlE →	ATP‑dependent RNA helicase RhlE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	831,857	0	A	G	100.0%	325.8 / NA	89	Y329C (TAT→TGT)	rhlE	ATP‑dependent RNA helicase RhlE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/44);  total (45/44)

GCGTACTCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTATGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATCCCGCGCATTGCGATTCC  >  NC_000913/831720‑831992                                                                                                                                          |                                                                                                                                        gcgTACTCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGGAGATtgtg                                                                                                                                       >  1:791012/1‑140 (MQ=255)     aCTCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCa                                                                                                                                   <  1:614951/140‑1 (MQ=255)      cTCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCAc                                                                                                                                  >  2:778163/1‑140 (MQ=255)       tCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCAcc                                                                                                                                 >  1:659402/1‑140 (MQ=255)       tCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCAcc                                                                                                                                 <  2:19281/140‑1 (MQ=255)        cgcgcgcTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCg                                                                                                                                >  2:1440657/4‑140 (MQ=255)        cGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCg                                                                                                                                >  2:1123098/1‑140 (MQ=255)        cGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCg                                                                                                                                >  2:975119/1‑140 (MQ=255)          tGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCa                                                                                                                                   >  1:1577402/1‑135 (MQ=255)          tGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTa                                                                                                                              <  2:542017/140‑1 (MQ=255)          tGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTAATGGTGGCAACTGACATCGCTGCGCGCGGCCTGCATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCa                                                                                                                                   <  2:1577402/135‑1 (MQ=255)               tggctgATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCggg                                                                                                                         <  2:1147933/140‑1 (MQ=255)                 gctgATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCg                                                                                                                       >  1:61930/1‑140 (MQ=255)                 gctgATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCg                                                                                                                       >  2:643600/1‑140 (MQ=255)                 gctgATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCg                                                                                                                       >  1:1112218/1‑140 (MQ=255)                 gctgATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCg                                                                                                                       >  1:226394/1‑140 (MQ=255)                              cGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCg                                                                                                          <  1:603855/140‑1 (MQ=255)                              cGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCg                                                                                                          <  1:1675829/140‑1 (MQ=255)                               gggCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCgg                                                                                                         >  1:919071/1‑140 (MQ=255)                                 gCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGgct                                                                                                       >  1:1462186/1‑140 (MQ=255)                                     tatTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTa                                                                                                   <  2:1462186/140‑1 (MQ=255)                                       ttCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTAcc                                                                                                 <  1:643600/140‑1 (MQ=255)                                          gtgtACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGt                                                                                              >  2:443811/1‑140 (MQ=255)                                             tACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGGAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGaa                                                                                           <  2:791012/140‑1 (MQ=255)                                               cTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGgc                                                                                                        <  2:1153050/125‑1 (MQ=255)                                               cTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGgc                                                                                                        >  1:1153050/1‑125 (MQ=255)                                               cTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGc                                                                                         >  2:1054993/1‑140 (MQ=255)                                               cTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGc                                                                                         >  2:1245414/1‑140 (MQ=255)                                               cTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGc                                                                                         >  1:1459806/1‑140 (MQ=255)                                                 ggtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       >  1:632725/1‑140 (MQ=255)                                                 ggtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       >  2:1385744/1‑140 (MQ=255)                                                 ggtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       >  1:1051352/1‑140 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGaa                                                                                           >  1:1661636/1‑135 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGaa                                                                                           <  2:1661636/135‑1 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGtt                                                                                      >  1:1417690/1‑140 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGtt                                                                                      >  1:1214103/1‑140 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGtt                                                                                      >  2:1240068/1‑140 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGtt                                                                                      >  2:1199018/1‑140 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       >  2:1366592/1‑139 (MQ=255)                                                  gtggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       <  1:1366592/139‑1 (MQ=255)                                                   tggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTg                                                                                     >  2:579771/1‑140 (MQ=255)                                                    ggCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGt                                                                                    <  2:1250066/140‑1 (MQ=255)                                                     gCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       <  1:23612/136‑1 (MQ=255)                                                     gCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGt                                                                                       >  2:23612/1‑136 (MQ=255)                                                      cAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCg                                                                                  <  1:1385744/140‑1 (MQ=255)                                                      cAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCg                                                                                  <  2:386441/140‑1 (MQ=255)                                                       aaCTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGc                                                                                 >  2:119876/1‑140 (MQ=255)                                                         cTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTg                                                                               <  2:1051352/140‑1 (MQ=255)                                                           gACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgt                                                                             <  1:1054993/140‑1 (MQ=255)                                                                cGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgtgtgtg                                                                        >  1:1494416/1‑140 (MQ=255)                                                                cGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgtgtgtg                                                                        <  2:1494416/140‑1 (MQ=255)                                                                    gcgcgcGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTtga                                                                    <  1:443811/140‑1 (MQ=255)                                                                     cgcgcgGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTtgat                                                                   <  2:1417690/140‑1 (MQ=255)                                                                      gcgcgGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgtgt                                                                           <  1:1552456/131‑1 (MQ=255)                                                                      gcgcgGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgtgt                                                                           >  2:1552456/1‑131 (MQ=255)                                                                      gcgcgGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgt                                                                             >  2:743553/1‑129 (MQ=255)                                                                      gcgcgGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGgt                                                                             <  1:743553/129‑1 (MQ=255)                                                                         cgGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAac                                                               <  1:119876/140‑1 (MQ=255)                                                                           gCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAacac                                                             <  2:659402/140‑1 (MQ=255)                                                                            ccTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAAcaca                                                            <  1:579771/140‑1 (MQ=255)                                                                              tGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACaaa                                                          <  1:1245414/140‑1 (MQ=255)                                                                              tGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACaaa                                                          <  2:226394/140‑1 (MQ=255)                                                                               ggATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAAc                                                         <  2:1214103/140‑1 (MQ=255)                                                                                   atTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAActgct                                                     <  1:1282167/140‑1 (MQ=255)                                                                                      gAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACaaa                                                          >  1:637109/1‑132 (MQ=255)                                                                                      gAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACaaa                                                          <  2:637109/132‑1 (MQ=255)                                                                                      gAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCg                                                  <  2:349321/140‑1 (MQ=255)                                                                                         gagCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGa                                               <  2:1112218/140‑1 (MQ=255)                                                                                         gagCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGa                                               <  1:1554907/140‑1 (MQ=255)                                                                                          agCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGat                                              >  2:70380/1‑140 (MQ=255)                                                                                            cTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGt                                                 >  2:269794/1‑135 (MQ=255)                                                                                            cTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGt                                                 <  1:269794/135‑1 (MQ=255)                                                                                            cTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGatat                                            >  2:195698/1‑140 (MQ=255)                                                                                            cTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGCGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGatat                                            >  1:993761/1‑140 (MQ=255)                                                                                              gCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCg                                          >  2:1378745/1‑140 (MQ=255)                                                                                                       cgtcAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAActgc                                 <  2:1175068/140‑1 (MQ=255)                                                                                                       cgtcAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAActgc                                 <  1:195698/140‑1 (MQ=255)                                                                                                        gtcAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAActgct                                <  2:61930/140‑1 (MQ=255)                                                                                                         tcAACTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGACGAACACAAACTGCTGCGTGATATCGAAAAActgctg                               >  2:575694/1‑140 (MQ=255)                                                                                                           aaCTATGAACTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGaa                             <  2:1459806/140‑1 (MQ=255)                                                                                                                  aaCTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAagag                      >  2:1560810/1‑140 (MQ=255)                                                                                                                  aaCTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAagag                      <  2:231123/140‑1 (MQ=255)                                                                                                                    cTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGAt                    <  1:975119/140‑1 (MQ=255)                                                                                                                    cTGCCAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGAt                    <  1:1123098/140‑1 (MQ=255)                                                                                                                       ccAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATccc                 >  2:796108/1‑140 (MQ=255)                                                                                                                       ccAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATccc                 >  2:990868/1‑140 (MQ=255)                                                                                                                       ccAAACGTACCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATccc                 >  1:598496/1‑140 (MQ=255)                                                                                                                               aCCTGAAGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATCCCGCGCATTg         <  1:990868/140‑1 (MQ=255)                                                                                                                                    aaGATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATCCCGCGCATTGCGAtt    >  2:1125972/1‑140 (MQ=255)                                                                                                                                      gATTGTGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATCCCGCGCATTGCGATTcc  >  2:1009290/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCGTACTCGTGCGCTGGCTGATTTTAAATCGGGCGATATTCGTGTACTGGTGGCAACTGACATCGCTGCGCGCGGCCTGGATATTGAAGAGCTGCCGCACGTCGTCAACTATGAACTGCCAAACGTACCTGAAGATTATGTCCACCGTATCGGGCGTACCGGTCGTGCGGCTGCTACCGGTGAAGCGTTGTCGCTGGTGTGTGTTGATGAACACAAACTGCTGCGTGATATCGAAAAACTGCTGAAAAAAGAGATCCCGCGCATTGCGATTCC  >  NC_000913/831720‑831992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑