Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,013,073	A→G	D358G (GAC→GGC)	pqiA →	intermembrane transport protein PqiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,013,073	0	A	G	100.0%	356.5 / NA	97	D358G (GAC→GGC)	pqiA	intermembrane transport protein PqiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/48);  total (49/48)

ATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTG  >  NC_000913/1012940‑1013201                                                                                                                                      |                                                                                                                                 atGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGtttt                                                                                                                            <  2:332530/140‑1 (MQ=255)  tGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTc                                                                                                                           >  1:810574/1‑140 (MQ=255)       ccAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTAt                                                                                                                      >  1:857656/1‑140 (MQ=255)       ccAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTAt                                                                                                                      >  1:1500439/1‑140 (MQ=255)       ccAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTAt                                                                                                                      >  1:164062/1‑140 (MQ=255)       ccAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTAt                                                                                                                      >  1:1096720/1‑140 (MQ=255)       ccAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTAt                                                                                                                      >  2:620456/1‑140 (MQ=255)       ccAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGCTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTAt                                                                                                                      >  1:957148/1‑140 (MQ=255)         aaCGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATcg                                                                                                                    >  2:1072956/1‑140 (MQ=255)             ttAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGt                                                                                                                <  1:1418308/140‑1 (MQ=255)                     gatCGCCATCGCGTGCCCGTGTTGGGATACCAACGGGCAAGACAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCgg                                                                                                        >  2:333380/1‑140 (MQ=255)                      atCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgc                                                                                                       >  1:711724/1‑140 (MQ=255)                      atCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgc                                                                                                       >  1:382573/1‑140 (MQ=255)                       tCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgcg                                                                                                      <  1:1229107/140‑1 (MQ=255)                       tCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgcg                                                                                                      <  1:682435/140‑1 (MQ=255)                        cGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgcgc                                                                                                     >  1:1074905/1‑140 (MQ=255)                          ccATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTg                                                                                                   <  1:902290/140‑1 (MQ=255)                          ccATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTg                                                                                                   <  1:525282/140‑1 (MQ=255)                          ccATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTg                                                                                                   <  2:894223/140‑1 (MQ=255)                          ccATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTg                                                                                                   <  1:1403375/140‑1 (MQ=255)                           cATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTgg                                                                                                  <  1:1236784/140‑1 (MQ=255)                            aTCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGt                                                                                                 <  1:949059/140‑1 (MQ=255)                             tCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGctct                                                                                                           >  2:1004491/1‑129 (MQ=255)                             tCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGctct                                                                                                           <  1:1004491/129‑1 (MQ=255)                             tCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTg                                                                                                >  2:1033042/1‑140 (MQ=255)                              cgcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgcg                                                                                                      <  2:43866/133‑1 (MQ=255)                              cgcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGgcg                                                                                                      >  1:43866/1‑133 (MQ=255)                                   ggCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATg                                                                                          <  2:1074905/140‑1 (MQ=255)                                    gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATgg                                                                                         <  1:635549/140‑1 (MQ=255)                                    gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATgg                                                                                         <  1:1072956/140‑1 (MQ=255)                                    gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATgg                                                                                         <  2:164062/140‑1 (MQ=255)                                    gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATgg                                                                                         <  1:405027/140‑1 (MQ=255)                                     cTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATggg                                                                                        <  2:857656/140‑1 (MQ=255)                                            gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTa                                                                                 >  1:670659/1‑140 (MQ=255)                                            gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTa                                                                                 <  2:382573/140‑1 (MQ=255)                                              gATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAAt                                                                               <  2:356618/140‑1 (MQ=255)                                               aTGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATg                                                                              >  1:1568185/1‑140 (MQ=255)                                                  ccAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGt                                                                           >  1:841679/1‑140 (MQ=255)                                                  ccAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGt                                                                           >  2:756447/1‑140 (MQ=255)                                                  ccAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGt                                                                           >  2:359284/1‑140 (MQ=255)                                                        ggCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTAt                                                                     <  1:645474/140‑1 (MQ=255)                                                        ggCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTAt                                                                     <  1:1308280/140‑1 (MQ=255)                                                        ggCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTAt                                                                     <  1:756447/140‑1 (MQ=255)                                                          cATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATcc                                                                   >  2:1013141/1‑140 (MQ=255)                                                           aTGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCg                                                                  >  2:239824/1‑140 (MQ=255)                                                            tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCgg                                                                 >  2:1446451/1‑140 (MQ=255)                                                            tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCgg                                                                 >  1:1205482/1‑140 (MQ=255)                                                            tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCgg                                                                 >  2:855145/1‑140 (MQ=255)                                                             gccAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGc                                                                <  2:1569701/138‑1 (MQ=255)                                                              gCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCa                                                               <  2:1120840/140‑1 (MQ=255)                                                              gCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCa                                                               <  1:359284/140‑1 (MQ=255)                                                                aaGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAAt                                                             <  1:1013141/140‑1 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTAt                                                                     >  2:451148/1‑130 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTAt                                                                     <  1:451148/130‑1 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATgg                                                           <  1:855145/140‑1 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATgg                                                           <  1:1592537/140‑1 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATgg                                                           <  2:810574/140‑1 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATgg                                                           <  2:292103/140‑1 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATgg                                                           >  2:1592537/1‑140 (MQ=255)                                                                  gcgcGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCAGCAATgg                                                           <  2:427784/140‑1 (MQ=255)                                                                       aCAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCa                                                      >  1:845844/1‑140 (MQ=255)                                                                       aCAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCa                                                      >  1:233451/1‑140 (MQ=255)                                                                             aaaGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATg                                                >  1:268157/1‑140 (MQ=255)                                                                               aGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGtt                                              <  1:239824/140‑1 (MQ=255)                                                                                   tGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCt                                          <  1:804631/140‑1 (MQ=255)                                                                                   tGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCt                                          >  1:1311019/1‑140 (MQ=255)                                                                                   tGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCt                                          >  2:1509870/1‑140 (MQ=255)                                                                                     cATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCttt                                        >  1:1157143/1‑140 (MQ=255)                                                                                     cATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCttt                                        >  2:1413727/1‑140 (MQ=255)                                                                                     cATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCttt                                        >  1:1522984/1‑140 (MQ=255)                                                                                        ttGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAgt                                     <  2:1157143/140‑1 (MQ=255)                                                                                         tGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAgtc                                    <  1:1033042/140‑1 (MQ=255)                                                                                           aTTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAgtcgt                                  <  2:268157/140‑1 (MQ=255)                                                                                            tttATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAgtcgtc                                 <  2:1568185/140‑1 (MQ=255)                                                                                             ttATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCa                                >  1:16686/1‑140 (MQ=255)                                                                                                  aaGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATg                           <  2:1248333/140‑1 (MQ=255)                                                                                                     ttgttgAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACa                        >  1:1448665/1‑140 (MQ=255)                                                                                                     ttgttgAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACa                        >  1:1032084/1‑140 (MQ=255)                                                                                                          gAGTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGtt                   <  2:711724/140‑1 (MQ=255)                                                                                                            gTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGtttt                 <  2:16686/140‑1 (MQ=255)                                                                                                            gTTTGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGtttt                 <  2:1032084/140‑1 (MQ=255)                                                                                                               tGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGtt                   >  2:222783/1‑135 (MQ=255)                                                                                                               tGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGtt                   <  1:222783/135‑1 (MQ=255)                                                                                                               tGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTg              >  2:570524/1‑140 (MQ=255)                                                                                                               tGTAGGCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTg              >  2:494259/1‑140 (MQ=255)                                                                                                                   ggCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTAt          <  1:1509870/140‑1 (MQ=255)                                                                                                                   ggCCGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTAt          <  2:1522984/140‑1 (MQ=255)                                                                                                                     ccGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGa        >  1:922917/1‑140 (MQ=255)                                                                                                                     ccGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGCGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGa        >  2:624605/1‑140 (MQ=255)                                                                                                                      cGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGAc       >  2:813015/1‑140 (MQ=255)                                                                                                                      cGCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGAc       >  2:1119132/1‑140 (MQ=255)                                                                                                                       gCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGa        >  2:55296/1‑138 (MQ=255)                                                                                                                       gCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGa        <  1:55296/138‑1 (MQ=255)                                                                                                                       gCTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACg      >  1:201827/1‑140 (MQ=255)                                                                                                                        cTGGTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGt     >  1:1412723/1‑140 (MQ=255)                                                                                                                           gTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACg      >  2:1139607/1‑136 (MQ=255)                                                                                                                           gTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACg      <  1:1139607/136‑1 (MQ=255)                                                                                                                           gTCGATGATTGGCGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTg  >  2:1268853/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 ATGGTGCCAACGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGATGATTGACGTTTTCGTTATCGCGGTGCTCTCGGCGCTGGTGCGTATGGGAGGTTTAATGAGTATTTATCCGGCAATGGGTGCATTAATGTTTGCTTTAGTCGTCATAATGACAATGTTTTCTGCTATGACGTTTG  >  NC_000913/1012940‑1013201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑