Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,083,115	C→T	F291F (TTC→TTT)	efeO →	ferrous iron transport system protein EfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,083,115	0	C	T	100.0%	184.7 / NA	52	F291F (TTC→TTT)	efeO	ferrous iron transport system protein EfeO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/27);  total (25/27)

GCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTCCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATACCGTAC  >  NC_000913/1082980‑1083249                                                                                                                                        |                                                                                                                                       gCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACGCCGATCTGTGGGATTTTCAgg                                                                                                                                    <  1:311758/140‑1 (MQ=255) gCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAgg                                                                                                                                    <  2:1160876/140‑1 (MQ=255) gCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAgg                                                                                                                                    <  2:239085/140‑1 (MQ=255) gCAAAAACGCA‑CAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGc                                                                                                                                   <  2:285068/140‑1 (MQ=255)   aaaaaCGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCt                                                                                                                                  <  1:387690/140‑1 (MQ=255)   aaaaaCGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCt                                                                                                                                  <  1:1400439/140‑1 (MQ=255)           aTCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGa                                                                                                                          <  1:515129/140‑1 (MQ=255)           aTCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGa                                                                                                                          <  1:1564528/140‑1 (MQ=255)                                 cTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGAt                                                                                                    >  1:1010113/1‑140 (MQ=255)                                 cTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGAt                                                                                                    <  2:296579/140‑1 (MQ=255)                                      aaaGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTtgct                                                                                               <  2:521377/140‑1 (MQ=255)                                           ggTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTc                                                                                          >  1:1155471/1‑140 (MQ=255)                                            gTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTcc                                                                                         >  2:862578/1‑140 (MQ=255)                                                 cggcgCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAAc                                                                                    >  2:1601100/1‑140 (MQ=255)                                                    cgcAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGc                                                                                 >  2:856024/1‑140 (MQ=255)                                                     gcAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCa                                                                                <  2:512581/140‑1 (MQ=255)                                                           ggACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGc                                                                          <  1:1601100/140‑1 (MQ=255)                                                                       gAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAAct                                                              >  1:1314653/1‑140 (MQ=255)                                                                        aaGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAActg                                                             <  1:541236/140‑1 (MQ=255)                                                                                 ccagcAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAActg                                                             >  2:887946/1‑131 (MQ=255)                                                                                 ccagcAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAActg                                                             <  1:887946/131‑1 (MQ=255)                                                                                   agcAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGt                                                  >  2:1257310/1‑140 (MQ=255)                                                                                     cAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCg                                                >  2:234995/1‑140 (MQ=255)                                                                                     cAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCg                                                >  1:892013/1‑140 (MQ=255)                                                                                          ttAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCACCCCGGAACTGCTGGCAAAAGTCGATGcc                                           <  2:1010113/140‑1 (MQ=255)                                                                                          ttAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGcc                                           <  2:338910/140‑1 (MQ=255)                                                                                           tAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCa                                          >  1:857981/1‑140 (MQ=255)                                                                                               ggTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACtt                                      >  2:1623512/1‑140 (MQ=255)                                                                                                   aagaagATCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTaaa                                  >  2:1626928/1‑140 (MQ=255)                                                                                                       agaTCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCATAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAgg                              >  1:1343286/1‑140 (MQ=255)                                                                                                        gaTCGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGt                             <  1:1257310/140‑1 (MQ=255)                                                                                                           cGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGa                          >  2:1036601/1‑140 (MQ=255)                                                                                                           cGCTACAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGa                          >  1:265636/1‑140 (MQ=255)                                                                                                                cAGCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCa                     >  1:1278502/1‑140 (MQ=255)                                                                                                                  gCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATa                        >  2:1336972/1‑135 (MQ=255)                                                                                                                  gCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATa                        <  1:1336972/135‑1 (MQ=255)                                                                                                                  gCCACACCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCAtt                   >  2:1675742/1‑140 (MQ=255)                                                                                                                     acacCGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTg                >  2:634577/1‑140 (MQ=255)                                                                                                                        ccGATCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCg             <  1:742960/140‑1 (MQ=255)                                                                                                                           aTCTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGaaa          <  2:892013/140‑1 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTc                  >  1:1008472/1‑130 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTc                  <  2:1008472/130‑1 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATa        <  1:234995/140‑1 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATa        <  2:265636/140‑1 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATa        >  1:65834/1‑140 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATa        <  2:847289/140‑1 (MQ=255)                                                                                                                             cTGTGGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATa        <  1:862578/140‑1 (MQ=255)                                                                                                                              tgtgGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCATAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATAc       <  2:1343286/140‑1 (MQ=255)                                                                                                                                tgGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGaa           <  1:184969/134‑1 (MQ=255)                                                                                                                                tgGGATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGaa           >  2:184969/1‑134 (MQ=255)                                                                                                                                 gggATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATACCGt    >  2:952001/1‑140 (MQ=255)                                                                                                                                   gATTTTCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATACCGTAc  >  1:1664834/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GCAAAAACGCATCAGTGAACTGGCTTTCCCACCTTCAAAAGTGGTCGGCGGCGCAGCCGGACTGATTGAGGAAGTGGCAGCCAGCAAAATTAGCGGTGAAGAAGATCGCTACAGCCACACCGATCTGTGGGATTTCCAGGCTAACGTTGAAGGCTCGCAGAAAATTGTCGATTTGCTGCGTCCACAACTGCAAAAAGCCAACCCGGAACTGCTGGCAAAAGTCGATGCCAACTTTAAAAAGGTCGATACCATTCTGGCGAAATACCGTAC  >  NC_000913/1082980‑1083249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑