Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,255,376	C→T	V193I (GTC→ATC)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,255,376	0	C	T	100.0%	272.1 / NA	76	V193I (GTC→ATC)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/46);  total (30/46)

TGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCC  >  NC_000913/1255241‑1255508                                                                                                                                        |                                                                                                                                     tGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  <  1:1084483/140‑1 (MQ=255) tGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  <  1:644834/140‑1 (MQ=255) tGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  <  1:322297/140‑1 (MQ=255) tGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  >  2:322297/1‑140 (MQ=255) tGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  <  1:1532200/140‑1 (MQ=255)   tAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  >  2:344952/1‑138 (MQ=255)   tAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTg                                                                                                                                  <  1:344952/138‑1 (MQ=255)   tAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGt                                                                                                                                <  2:64196/140‑1 (MQ=255)   tAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGt                                                                                                                                <  1:553758/140‑1 (MQ=255)      cccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGcc                                                                                                                             <  2:646519/140‑1 (MQ=255)       ccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCa                                                                                                                            <  1:1315955/140‑1 (MQ=255)       ccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCa                                                                                                                            <  1:516449/140‑1 (MQ=255)        cTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAA‑‑ACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAg                                                                                                                         >  2:649203/1‑140 (MQ=255)             cATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCa                                                                                                                      >  2:179081/1‑140 (MQ=255)                cGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTg                                                                                                                   >  2:1248590/1‑140 (MQ=255)                cGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTg                                                                                                                   <  2:58477/140‑1 (MQ=255)                cGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTg                                                                                                                   <  2:1313372/140‑1 (MQ=255)                cGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTg                                                                                                                   <  1:1218350/140‑1 (MQ=255)                   ttGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAA‑‑ACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:649203/140‑1 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:133586/140‑1 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:844907/140‑1 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:1020483/140‑1 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:486889/140‑1 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  2:199960/140‑1 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              >  2:486889/1‑140 (MQ=255)                     gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:1372964/140‑1 (MQ=255)                          gTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATa                                                                                                                          <  2:1336063/123‑1 (MQ=255)                          gTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATa                                                                                                                          >  1:1336063/1‑123 (MQ=255)                          gTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGc                                                                                                         <  1:1361825/140‑1 (MQ=255)                             tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              >  2:1397930/1‑132 (MQ=255)                             tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              >  1:1131400/1‑132 (MQ=255)                             tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  1:1397930/132‑1 (MQ=255)                             tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGt                                                                                                              <  2:1131400/132‑1 (MQ=255)                             tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCta                                                                                                      >  2:1580432/1‑140 (MQ=255)                             tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTAGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCta                                                                                                      <  2:850999/140‑1 (MQ=255)                                    gTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGtt                                                                                               >  2:787980/1‑140 (MQ=255)                                              cagcaCCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCa                                                                                     <  2:604332/140‑1 (MQ=255)                                              cagcaCCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGACCTATACCGTTCGAGTTTTCa                                                                                     <  2:21306/140‑1 (MQ=255)                                                             tATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTg                                                                      >  1:9121/1‑140 (MQ=255)                                                              aTTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTgg                                                                     <  2:1086662/140‑1 (MQ=255)                                                                  ccGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGt                                                                 <  1:1580432/140‑1 (MQ=255)                                                                         gaCCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGtt                                                          <  1:1674852/140‑1 (MQ=255)                                                                            caccGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAg                                                       <  2:424514/140‑1 (MQ=255)                                                                              ccGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCt                                                     <  1:520796/140‑1 (MQ=255)                                                                                 atatAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATg                                                  <  1:787980/140‑1 (MQ=255)                                                                                 atatAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATg                                                  <  1:644353/140‑1 (MQ=255)                                                                                         acCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCaataa                                          >  1:153760/1‑140 (MQ=255)                                                                                         acCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCaataa                                          >  1:1392439/1‑140 (MQ=255)                                                                                           cTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatg                                        <  2:1392439/140‑1 (MQ=255)                                                                                           cTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatg                                        >  1:1618957/1‑140 (MQ=255)                                                                                           cTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatg                                        >  2:1224001/1‑140 (MQ=255)                                                                                            tGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAg                                                       <  2:534389/124‑1 (MQ=255)                                                                                            tGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAg                                                       >  1:534389/1‑124 (MQ=255)                                                                                            tGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatga                                       >  2:452281/1‑140 (MQ=255)                                                                                            tGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatga                                       >  2:1034356/1‑140 (MQ=255)                                                                                            tGTACTTACATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatga                                       >  2:289454/1‑140 (MQ=255)                                                                                              tACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatgatg                                     >  2:1089064/1‑140 (MQ=255)                                                                                              tACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAatgatg                                     >  2:1024320/1‑140 (MQ=255)                                                                                                  tCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGt                                 <  1:1089064/140‑1 (MQ=255)                                                                                                   ccATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTc                                >  2:15998/1‑140 (MQ=255)                                                                                                     aTCGGTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCAt                              >  2:126438/1‑140 (MQ=255)                                                                                                        ggTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCg                            <  2:228813/139‑1 (MQ=255)                                                                                                        ggTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCg                            >  1:228813/1‑139 (MQ=255)                                                                                                        ggTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGt                           >  2:268840/1‑140 (MQ=255)                                                                                                        ggTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGt                           <  1:1248590/140‑1 (MQ=255)                                                                                                        ggTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGt                           <  1:289454/140‑1 (MQ=255)                                                                                                        ggTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGt                           <  2:590407/140‑1 (MQ=255)                                                                                                         gTCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGtt                          <  1:179081/140‑1 (MQ=255)                                                                                                          tCGTGATTTTACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTa                         >  1:776750/1‑140 (MQ=255)                                                                                                                 tttACTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTaa                  <  1:1224001/140‑1 (MQ=255)                                                                                                                    aCTTCCGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTTAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATgg               >  2:36869/1‑140 (MQ=255)                                                                                                                         cGCTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGtgctg          >  2:425358/1‑140 (MQ=255)                                                                                                                           cTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGcc        <  1:287007/140‑1 (MQ=255)                                                                                                                           cTTCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGcc        >  2:287007/1‑140 (MQ=255)                                                                                                                            ttCCAAGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCt       <  2:1618957/140‑1 (MQ=255)                                                                                                                                 aGATCGATACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGcc  <  2:776750/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCC  >  NC_000913/1255241‑1255508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑