Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,521,796	A→G	F424L (TTT→CTT)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,521,796	0	A	G	100.0%	284.1 / NA	78	F424L (TTT→CTT)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/33);  total (45/33)

TCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCG  >  NC_000913/1521659‑1521926                                                                                                                                          |                                                                                                                                   tCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGcc                                                                                                                                  >  1:1542663/1‑140 (MQ=255)  cGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCca                                                                                                                                 >  1:444646/1‑140 (MQ=255)   gCCCGGCTGCCAGATAGATATTCCAGACATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCcac                                                                                                                                >  2:930022/1‑140 (MQ=255)    cccGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCcaca                                                                                                                               <  1:1060209/140‑1 (MQ=255)    cccGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCcaca                                                                                                                               <  2:304765/140‑1 (MQ=255)    cccGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCcaca                                                                                                                               <  2:1465378/140‑1 (MQ=255)    cccGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCcaca                                                                                                                               <  2:1542663/140‑1 (MQ=255)                 tagataTTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAgcg                                                                                                                  <  2:1014088/140‑1 (MQ=255)                      atTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGc                                                                                                             >  1:577415/1‑140 (MQ=255)                      atTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGc                                                                                                             >  2:588511/1‑140 (MQ=255)                        tCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAgcg                                                                                                                  >  2:25002/1‑133 (MQ=255)                        tCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAgcg                                                                                                                  <  1:25002/133‑1 (MQ=255)                         ccAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAg                                                                                                          <  2:970435/140‑1 (MQ=255)                            ggCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCAt                                                                                                       >  2:1250738/1‑140 (MQ=255)                                 cGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGc                                                                                                  >  1:74246/1‑140 (MQ=255)                                 cGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGc                                                                                                  >  2:84698/1‑140 (MQ=255)                                  ggTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCg                                                                                                 >  2:672262/1‑140 (MQ=255)                                  ggTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCg                                                                                                 >  1:1080671/1‑140 (MQ=255)                                  ggTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCg                                                                                                 >  2:905628/1‑140 (MQ=255)                                       ttGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGt                                                                                            >  2:1574264/1‑140 (MQ=255)                                            tatTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTc                                                                                       <  1:1574264/140‑1 (MQ=255)                                             atTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCg                                                                                      >  1:442718/1‑140 (MQ=255)                                                 tAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCa                                                                                  >  1:445673/1‑140 (MQ=255)                                                  aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 >  1:1157425/1‑140 (MQ=255)                                                  aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 <  2:74246/140‑1 (MQ=255)                                                  aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 <  2:1626689/140‑1 (MQ=255)                                                  aaCGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 >  1:897333/1‑140 (MQ=255)                                                       aCCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGcc                                                                            >  2:179185/1‑140 (MQ=255)                                                        ccGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCa                                                                           >  2:462174/1‑140 (MQ=255)                                                           gCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTg                                                                        <  1:328796/140‑1 (MQ=255)                                                            cAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 >  1:935172/1‑130 (MQ=255)                                                            cAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 <  2:935172/130‑1 (MQ=255)                                                            cAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGc                                                                       <  2:577415/140‑1 (MQ=255)                                                             aGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCg                                                                      <  1:1122800/140‑1 (MQ=255)                                                               gTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTc                                                                    <  1:462174/140‑1 (MQ=255)                                                                 aGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGt                                                                     <  1:1528466/137‑1 (MQ=255)                                                                 aGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGt                                                                     >  2:1528466/1‑137 (MQ=255)                                                                  gCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTg                                                                        <  1:1555098/133‑1 (MQ=255)                                                                  gCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTg                                                                        >  2:1555098/1‑133 (MQ=255)                                                                  gCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGt                                                                 <  1:905628/140‑1 (MQ=255)                                                                  gCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGt                                                                 <  1:820305/140‑1 (MQ=255)                                                                   ccATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 >  2:1638102/1‑123 (MQ=255)                                                                   ccATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAg                                                                                 <  1:1638102/123‑1 (MQ=255)                                                                     aTTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTaaa                                                              <  2:442718/140‑1 (MQ=255)                                                                       ttATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATa                                                            <  1:1250738/140‑1 (MQ=255)                                                                         aTGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAgg                                                          >  2:1153535/1‑140 (MQ=255)                                                                          tGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAggg                                                         <  1:672262/140‑1 (MQ=255)                                                                           gATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGt                                                                     >  1:1651508/1‑127 (MQ=255)                                                                           gATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGt                                                                     <  2:1651508/127‑1 (MQ=255)                                                                             tAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATc                                                      >  2:942054/1‑140 (MQ=255)                                                                               aCTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGa                                                    >  1:650012/1‑140 (MQ=255)                                                                               aCTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGa                                                    <  1:942054/140‑1 (MQ=255)                                                                                cTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGAt                                                   >  2:1337359/1‑140 (MQ=255)                                                                                   gCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt                                                <  1:179185/140‑1 (MQ=255)                                                                                   gCATCACCGCTGTCATCGCCGTTACCCGGAGTAACATAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt                                                >  2:717579/1‑140 (MQ=255)                                                                                    cATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt                                                <  1:140848/139‑1 (MQ=255)                                                                                    cATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt                                                >  2:140848/1‑139 (MQ=255)                                                                                    cATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTc                                               >  1:1113756/1‑140 (MQ=255)                                                                                     aTCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTcc                                              >  2:1129539/1‑140 (MQ=255)                                                                                     aTCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTcc                                              >  2:1165202/1‑140 (MQ=255)                                                                                              gTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGAt                                     >  1:100476/1‑140 (MQ=255)                                                                                                    gCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt                                                <  1:1449229/123‑1 (MQ=255)                                                                                                    gCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGtt                                                >  2:1449229/1‑123 (MQ=255)                                                                                                    gCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTCCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCGGATTGCGtt                               >  2:527875/1‑140 (MQ=255)                                                                                                            ccGGAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGa                       <  1:84698/140‑1 (MQ=255)                                                                                                              ggAGTAACGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACg                     <  2:1157425/140‑1 (MQ=255)                                                                                                              ggAGTAACATAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACg                     <  1:717579/140‑1 (MQ=255)                                                                                                                   aaCGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACt                <  2:897333/140‑1 (MQ=255)                                                                                                                    aCGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTc               >  2:1510486/1‑140 (MQ=255)                                                                                                                    aCGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTc               <  2:1113756/140‑1 (MQ=255)                                                                                                                     cGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGTATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCa              >  2:1575949/1‑140 (MQ=255)                                                                                                                     cGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCa              >  2:1379475/1‑140 (MQ=255)                                                                                                                     cGTAATGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCa              >  2:944189/1‑140 (MQ=255)                                                                                                                          tGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCttt         >  2:1343397/1‑140 (MQ=255)                                                                                                                          tGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCttt         >  1:1108530/1‑140 (MQ=255)                                                                                                                          tGGTCGTTGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCttt         >  1:1008491/1‑140 (MQ=255)                                                                                                                                ttGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGccc   >  2:146424/1‑140 (MQ=255)                                                                                                                                 tGGAATCAAGCCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCg  >  2:1624940/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   TCGCCCGGCTGCCAGATAGATATTCCAGGCATCGGTCATTGCATATTTTAACGAACCGGCAGGTAGCCATTTATGATAACTGGCATCACCGCTGTCATCGCCGTTACCCGGAGTAACGTAATGGTCGTTGGAATCAAACCACACGGAGCTGTAGCGCACGCCAGCATCCAGCGACAGTTTTTCGCTCAGCTGCCACTGCGTCTGTAAATAGGGATCGATGTTCCACATCAGATTGCGTTCGTCGCGACGCAACTCACCTTTTTGCCCG  >  NC_000913/1521659‑1521926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑