Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,609,419	T→C	Y421C (TAT→TGT)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,419	0	T	C	100.0%	252.7 / NA	69	Y421C (TAT→TGT)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/47);  total (22/47)

GTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAG  >  NC_000913/1609283‑1609555                                                                                                                                         |                                                                                                                                         gTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAAc                                                                                                                                       >  2:1019637/1‑140 (MQ=255) gTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAAc                                                                                                                                       <  2:806100/140‑1 (MQ=255)      tAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCT‑GCTCCAGAGTTGCTGACAACGTTcca                                                                                                                                 <  2:406334/140‑1 (MQ=255)        gCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccag                                                                                                                                <  1:1313157/140‑1 (MQ=255)        gCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccag                                                                                                                                <  2:53089/140‑1 (MQ=255)         cAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccagc                                                                                                                               <  1:1576711/140‑1 (MQ=255)          aaCCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccagcc                                                                                                                              <  1:374227/140‑1 (MQ=255)           aCCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccagcca                                                                                                                             >  1:898119/1‑140 (MQ=255)           aCCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccagcca                                                                                                                             <  1:28858/140‑1 (MQ=255)           aCCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTccagcca                                                                                                                             <  1:1080597/140‑1 (MQ=255)               gCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAgtgt                                                                                                                         <  1:332217/140‑1 (MQ=255)                cTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGtgtg                                                                                                                        >  1:1458938/1‑140 (MQ=255)                cTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGtgtg                                                                                                                        <  1:269225/140‑1 (MQ=255)                  cGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCa                                                                                                                      <  1:43076/140‑1 (MQ=255)                  cGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCa                                                                                                                      <  2:1428898/140‑1 (MQ=255)                  cGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTATTTTTCTGCCAAAACAAGCGCTACCAGTTCCTGGGTGCCGATAACACGGTCGCGATGCTGGCTCCAGATTTGCTGACAACGTTCCAGCCAGTGTGCa                                                                                                                      <  2:1579375/140‑1 (MQ=255)                   gaccagaccaGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCAt                                                                                                                     <  1:978862/140‑1 (MQ=255)                       agaccagGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTc                                                                                                                 <  1:1191621/140‑1 (MQ=255)                       agaccagGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTc                                                                                                                 <  1:1592791/140‑1 (MQ=255)                       agaccagGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTc                                                                                                                 <  2:1458938/140‑1 (MQ=255)                        gaccagGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTcc                                                                                                                <  2:1629884/140‑1 (MQ=255)                           cagGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGt                                                                                                             <  1:518667/140‑1 (MQ=255)                                     cGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATg                                                                                                   <  1:1019637/140‑1 (MQ=255)                                            tCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGcc                                                                                            >  1:810472/1‑140 (MQ=255)                                             ccTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCg                                                                                           <  2:303502/140‑1 (MQ=255)                                              cTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGt                                                                                          >  1:1634222/1‑140 (MQ=255)                                                gCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTa                                                                                        <  1:993229/140‑1 (MQ=255)                                                  tCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      <  2:1403472/140‑1 (MQ=255)                                                  tCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      <  2:794107/140‑1 (MQ=255)                                                  tCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      <  2:968725/140‑1 (MQ=255)                                                  tCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      <  1:1028920/140‑1 (MQ=255)                                                    ccAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCt                                                                                    <  1:942271/140‑1 (MQ=255)                                                    ccAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCt                                                                                    <  2:1479109/140‑1 (MQ=255)                                                      aGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      <  2:341759/136‑1 (MQ=255)                                                      aGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      >  1:341759/1‑136 (MQ=255)                                                      aGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTca                                                                                  >  1:975306/1‑140 (MQ=255)                                                       gTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTcac                                                                                 <  2:975306/140‑1 (MQ=255)                                                           tCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      <  2:4104/131‑1 (MQ=255)                                                           tCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACg                                                                                      >  1:4104/1‑131 (MQ=255)                                                             tttttCTGCCAAAACAATCGCTACCAGTTCCTGGTTGCCGATAACACGGTCACGACGCTAGCTCCAGAGTAGCTGACAACGCTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGCTTCCCCGTTACGCTCACCACGAt                                                                           >  2:455314/1‑140 (MQ=255)                                                              ttttCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATa                                                                          <  2:898119/140‑1 (MQ=255)                                                                          aCAATCGCTCCCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAGCGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGcaatcaa                                                              <  2:916999/140‑1 (MQ=255)                                                                          aCAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGcaatcaa                                                              <  1:1328437/140‑1 (MQ=255)                                                                          aCAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGcaatcaa                                                              <  1:1332920/140‑1 (MQ=255)                                                                                 cTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCt                                                       >  2:1484130/1‑140 (MQ=255)                                                                                  tACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTa                                                      <  1:1484130/140‑1 (MQ=255)                                                                                     cAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCTTCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATg                                                   <  2:630109/140‑1 (MQ=255)                                                                                     cAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATg                                                   <  1:500272/140‑1 (MQ=255)                                                                                          ccTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGaaa                                              >  1:913243/1‑140 (MQ=255)                                                                                                tGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAgg                                        <  2:913243/140‑1 (MQ=255)                                                                                                  ccGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGc                                                        <  1:626810/122‑1 (MQ=255)                                                                                                  ccGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGc                                                        >  2:626810/1‑122 (MQ=255)                                                                                                  ccGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGgcg                                      >  2:889147/1‑140 (MQ=255)                                                                                                     aTAACACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGgcgcgc                                   <  2:676838/140‑1 (MQ=255)                                                                                                       aaCACGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCg                                 <  1:179118/140‑1 (MQ=255)                                                                                                        acacGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCgg                                >  2:1401936/1‑140 (MQ=255)                                                                                                        acacGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCgg                                >  2:742901/1‑140 (MQ=255)                                                                                                         cacGGTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGa                               <  1:889147/140‑1 (MQ=255)                                                                                                         cacGGTCACGATGCTGGCTCCAGAGTTGCTCACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGa                               >  2:371238/1‑140 (MQ=255)                                                                                                             gTCACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAgtg                           >  1:580851/1‑140 (MQ=255)                                                                                                               cACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAgg                                        >  1:516278/1‑125 (MQ=255)                                                                                                               cACGATGCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAgg                                        <  2:516278/125‑1 (MQ=255)                                                                                                                     gCTGGCTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGttt                   <  1:1401936/140‑1 (MQ=255)                                                                                                                          cTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCtt              >  1:1392719/1‑140 (MQ=255)                                                                                                                          cTCCAGAGTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCtt              >  1:997555/1‑140 (MQ=255)                                                                                                                              agagTTGCTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTctgc          <  2:1392719/140‑1 (MQ=255)                                                                                                                                     cTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTctgcc         <  1:1523792/134‑1 (MQ=255)                                                                                                                                     cTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTctgcc         >  2:1523792/1‑134 (MQ=255)                                                                                                                                     cTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCca   >  1:1449777/1‑140 (MQ=255)                                                                                                                                     cTGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCca   >  2:1620679/1‑140 (MQ=255)                                                                                                                                      tGACAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCTCCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCcag  <  2:1166237/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAG  >  NC_000913/1609283‑1609555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑