Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,684,740	T→C	V161A (GTT→GCT)	tus →	DNA replication terminus site‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,740	0	T	C	100.0%	75.9 / NA	22	V161A (GTT→GCT)	tus	DNA replication terminus site‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/0);  total (22/0)

CACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAG  >  NC_000913/1684604‑1684876                                                                                                                                         |                                                                                                                                         cacaTCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGctct                                                                                                                                       >  1:865574/1‑140 (MQ=255)      cAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACg                                                                                                                                  >  1:1456090/1‑140 (MQ=255)              tCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGcc                                                                                                                          >  1:539763/1‑140 (MQ=255)                aaGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCAc                                                                                                                        >  1:848953/1‑140 (MQ=255)                       cGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGc                                                                                                                 >  1:774796/1‑140 (MQ=255)                               aatttCGTCAGGTTTGAACCAACCCCCCCCCCCGCGGCACTTTTTGAGTGGGTGCATCGTCTTTTGCGGGGGCTGACCCCCTTTAATGCTCCCCGCCCGCCCCCCGCTCTGCGCGCCCCCGCCACTTTACGCTttggttg                                                                                                         <  2:823443/136‑1 (MQ=255)                               aatatCGCCACGGTTAAATCAGACCCCCCCCCCGCGGCACTTTTTGAATGGGTGCATCGTCTTTTGCCGGGGCTGACCCCCTTTAATGCTTCCCCCCCGCCCCCCGCTCTGCACGCCCCCGCCACTTTACGCTttggttg                                                                                                         <  2:925454/139‑1 (MQ=255)                                 tgtCGTCAGGGTTGAAACAAACCTCCCCACCGCGGCGTGTTTTGAATGGGTACTTCGTCATTTGCGGGGGCTAATCCCCTTTATTGCTTACCGCACGCTCACCCCTCCGCACGCCCCCGCCACTTTACGCTTTGGTTggg                                                                                                       <  2:514301/138‑1 (MQ=255)                                 tatCGTCGGGGTTGAATCAGACCCCCCCCCCGCGGCCTTTTTTGAATGGGTGCCTCGTCTTTTGCGGGGGCTGACCCCCTTTAATGCTTCCCGCCCGCCCCCCGCTCTCCACGCCCCCGCCACTTTACGCTTTGGTTggg                                                                                                       <  2:1512375/140‑1 (MQ=255)                                  atCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGc                                                                                                      >  1:823443/1‑140 (MQ=255)                                        aCGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCtaataa                                                                                                >  1:1512375/1‑140 (MQ=255)                                        aCGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCtaataa                                                                                                >  1:1214624/1‑140 (MQ=255)                                            ttGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACat                                                                                            >  1:553874/1‑140 (MQ=255)                                                   agacccccccccCGCGGCACTTTTTGAAGGGGTGCATCGTCTTTGGCGGGGGCTGATCCCCTTTAATGCTTCCCGCACGCTCCCCGCTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTa                                                                                     <  2:1456090/134‑1 (MQ=255)                                                    gAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTaa                                                                                    >  1:804579/1‑140 (MQ=255)                                                     acccccccACCGCGGCGCTTTTTAAAGGGGGGCAGCGTCATTGGCGGGGGCTGATCACCTTTAATTCTTCCGTCACGCGCCCCTCTCTCCAGGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAg                                                                                   <  2:898387/136‑1 (MQ=255)                                                               cgcgGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATc                                                                         >  1:46514/1‑140 (MQ=255)                                                                   gCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCATTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtga                                                                     >  2:934870/1‑140 (MQ=255)                                                                      cGTTTTGAAGGGGTGCATCGTCATTTGCCGGGGCTGATCCCCTTTAATGCTTCCCGCACGCCCCCCGCTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga                                                                  <  2:553874/140‑1 (MQ=255)                                                                        ttttGAATGGGTGCATCGTCATTTGCCGGGGCTGACCCCCTTTAATGCTTCCCGCCCGCTCCCCGCTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAg                                                                <  2:539763/140‑1 (MQ=255)                                                                                       tCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTgg                                                 >  2:1549483/1‑140 (MQ=255)                                                                                                gCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGcc                                        >  1:1579021/1‑140 (MQ=255)                                                                                                     ggCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGaaa                                   >  1:934069/1‑140 (MQ=255)                                                                                                     ggCTGATCACCCTTAATGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGaaa                                   >  1:509694/1‑140 (MQ=255)                                                                                                             ccctttAATGCTTCCCGCCCGCCCCCCGCTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACg                           <  2:668049/136‑1 (MQ=255)                                                                                                             ccctttAATCCTCCCCGCCCCCACACCCCTCTCCACCCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACg                           <  2:46514/136‑1 (MQ=255)                                                                                                             ccccTTAATGCTTCCCGCCCGCCCCCCGCTCTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACg                           <  2:865574/139‑1 (MQ=255)                                                                                                             ccccTTAATGCTTCCCCCCCGCCCCCCGCTCTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACg                           <  2:774796/139‑1 (MQ=255)                                                                                                             ccccTTAATGCTTACCGCCCGCCCCCCGCTCTCCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACg                           <  2:848953/139‑1 (MQ=255)                                                                                                                    aTGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATcacc                              >  2:1151109/1‑130 (MQ=255)                                                                                                                    aTGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATcacc                              <  1:1151109/130‑1 (MQ=255)                                                                                                                    aTGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTc                    >  1:831628/1‑140 (MQ=255)                                                                                                                     tGCTTACCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCg                   >  2:797735/1‑140 (MQ=255)                                                                                                                          aCCGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGATAAAAGCCTGAAATCACCAGGCATTGTCGCACCg              >  2:1468463/1‑140 (MQ=255)                                                                                                                            cGCACGCTCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTg            >  1:17951/1‑140 (MQ=255)                                                                                                                                   tCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGAc         <  2:528975/136‑1 (MQ=255)                                                                                                                                   tCACCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGAc         >  1:528975/1‑136 (MQ=255)                                                                                                                                     aCCGCTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCga   >  1:1171488/1‑140 (MQ=255)                                                                                                                                      ccGCTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCgag  <  1:1065954/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAG  >  NC_000913/1684604‑1684876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑