Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,154,161	G→A	G49D (GGT→GAT)	mdtA →	multidrug efflux pump membrane fusion protein MdtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,154,161	0	G	A	100.0%	116.5 / NA	34	G49D (GGT→GAT)	mdtA	multidrug efflux pump membrane fusion protein MdtA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/9);  total (25/9)

GTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGGTGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCG  >  NC_000913/2154026‑2154295                                                                                                                                        |                                                                                                                                       gTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatggt                                                                                                                                    <  2:141150/140‑1 (MQ=255)  ttATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTc                                                                                                                                   >  2:1557562/1‑140 (MQ=255)      aaaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACg                                                                                                                               <  1:1557562/140‑1 (MQ=255)       aaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatggt                                                                                                                                    >  2:1360397/1‑134 (MQ=255)       aaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatggt                                                                                                                                    <  1:1360397/134‑1 (MQ=255)       aaTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGt                                                                                                                              <  2:990999/140‑1 (MQ=255)        aTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTg                                                                                                                             >  2:426911/1‑140 (MQ=255)        aTCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTg                                                                                                                             >  2:927563/1‑140 (MQ=255)         tCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGt                                                                                                                              >  1:1401508/1‑138 (MQ=255)         tCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGt                                                                                                                              <  2:1401508/138‑1 (MQ=255)         tCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTgg                                                                                                                            >  2:360743/1‑140 (MQ=255)            cGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTAt                                                                                                                         >  2:1591384/1‑140 (MQ=255)            cGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTAt                                                                                                                         >  1:868231/1‑140 (MQ=255)               tGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatgg                                                                                                                                     >  1:152575/1‑125 (MQ=255)               tGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGgatgg                                                                                                                                     <  2:152575/125‑1 (MQ=255)                gggTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGt                                                                                                                     <  1:1591384/140‑1 (MQ=255)                   taatcgtaatcgTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTcc                                                                                                                  <  1:927563/140‑1 (MQ=255)                         taatcgtGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCa                                                                                                            <  1:1476928/140‑1 (MQ=255)                               tggtggTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGcc                                                                                                      <  1:426911/140‑1 (MQ=255)                                ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:1159880/1‑140 (MQ=255)                                ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:879877/1‑140 (MQ=255)                                ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:792659/1‑140 (MQ=255)                                ggtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGccc                                                                                                     >  1:1380126/1‑140 (MQ=255)                                 gtggtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGcccc                                                                                                    >  1:61037/1‑140 (MQ=255)                                    gtTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGt                                                                                                 >  1:410620/1‑140 (MQ=255)                                     ttATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGtt                                                                                                <  1:360743/140‑1 (MQ=255)                                           ccgccATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAggcg                                                                                          <  2:1357089/140‑1 (MQ=255)                                              ccATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGgcggcg                                                                                       >  1:13779/1‑140 (MQ=255)                                                acccccgcATTCTGGTTCGGGCAAGGCCGAAATGCCCCCCGGGGGGCAGCCCGGGGGGCGACAAAACAAGCGCAGCAACCGCCAGGGGATGGTCGACGTGGTATGCGTCCCGCCCCATTAGCCCCGTTTCAGGCGGCGAc                                                                                     <  2:792659/136‑1 (MQ=255)                                                                      aaGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCcg                                                               >  2:968469/1‑140 (MQ=255)                                                                                tgccccccGGAGTGCAGCCCCGGGGGCGACAAAACAAGCGCACCAACCGCCAGGGGATGGTCGACGTGGTATGCGTCCCGGCCCATTACCCCCGGTTCGGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCa                                                     <  2:1488806/135‑1 (MQ=255)                                                                                  aCTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCAcc                                                   >  1:1488806/1‑140 (MQ=255)                                                                                  aCTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCAcc                                                   >  1:755332/1‑140 (MQ=255)                                                                                  aCTCCCAGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCAcc                                                   >  1:866473/1‑140 (MQ=255)                                                                                    tCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCgg                                                 >  2:341113/1‑140 (MQ=255)                                                                                              cAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCAcc                                       >  1:576212/1‑140 (MQ=255)                                                                                              cAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCAcc                                       >  1:434646/1‑140 (MQ=255)                                                                                                      ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGc                                               >  1:918304/1‑124 (MQ=255)                                                                                                      ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGc                                               <  2:918304/124‑1 (MQ=255)                                                                                                      ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTAccgc                               >  1:1568914/1‑140 (MQ=255)                                                                                                      ggggCGACGAAACAAGCGCAGCAATCGCCAGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTAccgc                               >  1:1306808/1‑140 (MQ=255)                                                                                                                                   aGCGGATGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTACCGCCGCTAATACCGTTACGGTGCGCAGCcgcg  >  1:112611/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GTTATAAATCCCGTTGGGTAATCGTAATCGTGGTGGTTATCGCCGCCATCGCCGCATTCTGGTTCTGGCAAGGCCGCAATGACTCCCGGAGTGCAGCCCCAGGGGCGACGAAACAAGCGCAGCAATCGCCAGCGGGTGGTCGACGTGGTATGCGTTCCGGCCCATTAGCCCCGGTTCAGGCGGCGACCGCCGTAGAACAGGCAGTTCCGCGTTACCTCACCGGGCTTGGCACCATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCG  >  NC_000913/2154026‑2154295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑