Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,171,849	A→G	V342A (GTG→GCG)	gatD ←	galactitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,171,849	0	A	G	100.0%	248.0 / NA	68	V342A (GTG→GCG)	gatD	galactitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/31);  total (37/31)

GCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCC  >  NC_000913/2171712‑2171977                                                                                                                                          |                                                                                                                                 gCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCt                                                                                                                                >  2:895615/1‑140 (MQ=255)  cTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCtt                                                                                                                               >  1:1571903/1‑140 (MQ=255)     gaatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGc                                                                                                                            <  1:395632/140‑1 (MQ=255)      aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGcc                                                                                                                           <  1:1211277/140‑1 (MQ=255)      aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGcc                                                                                                                           <  1:1271997/140‑1 (MQ=255)      aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGcc                                                                                                                           <  2:435747/140‑1 (MQ=255)          aatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGc                                                                                                                       <  2:1571903/140‑1 (MQ=255)          aatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGc                                                                                                                       <  1:700231/140‑1 (MQ=255)            ttCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCAt                                                                                                                     >  2:617422/1‑140 (MQ=255)               aTCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGc                                                                                                                  >  2:153442/1‑140 (MQ=255)                 cGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCAt                                                                                                                >  1:948902/1‑140 (MQ=255)                    cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGc                                                                                                                       <  2:281869/130‑1 (MQ=255)                    cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGc                                                                                                                       >  1:281869/1‑130 (MQ=255)                    cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTAc                                                                                                             <  2:915951/140‑1 (MQ=255)                      tttACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGa                                                                                                           >  1:125057/1‑140 (MQ=255)                        tACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGc                                                                                                                       <  2:1376212/126‑1 (MQ=255)                        tACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGc                                                                                                                       >  1:1376212/1‑126 (MQ=255)                         aCCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCAt                                                                                                                     <  2:814726/127‑1 (MQ=255)                         aCCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCAt                                                                                                                     >  1:814726/1‑127 (MQ=255)                              tatTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTc                                                                                                   >  2:1448686/1‑140 (MQ=255)                               atTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAg                                                                                                          <  1:1674510/132‑1 (MQ=255)                               atTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAg                                                                                                          >  2:1674510/1‑132 (MQ=255)                                    aTTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCAc                                                                                             >  2:548826/1‑140 (MQ=255)                                     ttGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGt                                                                                                    <  1:1486273/132‑1 (MQ=255)                                     ttGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGt                                                                                                    >  2:1486273/1‑132 (MQ=255)                                        tgGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGcc                                                                                         >  1:523181/1‑140 (MQ=255)                                         ggCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCt                                                                                        >  2:140964/1‑140 (MQ=255)                                          gCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTg                                                                                       >  2:1037079/1‑140 (MQ=255)                                             aGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGc                                                                                    >  2:1240710/1‑140 (MQ=255)                                               tgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGa                                                                                  >  1:852204/1‑140 (MQ=255)                                                        aGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCaa                                                                         <  1:1240710/140‑1 (MQ=255)                                                         gggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCaaa                                                                        >  2:695786/1‑140 (MQ=255)                                                         gggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCaaa                                                                        >  2:106941/1‑140 (MQ=255)                                                              aCCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTc                                                                   >  1:320277/1‑140 (MQ=255)                                                               ccATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTcc                                                                  >  2:790134/1‑140 (MQ=255)                                                                cATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCa                                                                 <  2:852204/140‑1 (MQ=255)                                                                  ttGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACg                                                               >  2:901889/1‑140 (MQ=255)                                                                  ttGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACg                                                               <  2:125057/140‑1 (MQ=255)                                                                      tGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCTGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGa                                                           <  2:1203129/140‑1 (MQ=255)                                                                       gAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAg                                                          <  2:320277/140‑1 (MQ=255)                                                                        aaTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGc                                                         >  1:1103947/1‑140 (MQ=255)                                                                          tGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGa                                                       >  2:368532/1‑140 (MQ=255)                                                                            aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCA‑TACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTa                                                    >  1:246905/1‑140 (MQ=255)                                                                               tGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAAt                                                  <  2:1016879/140‑1 (MQ=255)                                                                               tGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAAt                                                  <  1:617422/140‑1 (MQ=255)                                                                                   ttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                              <  2:948902/140‑1 (MQ=255)                                                                                   ttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                              <  1:993112/140‑1 (MQ=255)                                                                                    tGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                             >  2:1138202/1‑140 (MQ=255)                                                                                      cacaATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCa                                           >  1:711700/1‑140 (MQ=255)                                                                                        caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAgg                                         <  1:892582/140‑1 (MQ=255)                                                                                        caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAgg                                         <  1:695786/140‑1 (MQ=255)                                                                                         aaTACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGc                                        <  2:523181/140‑1 (MQ=255)                                                                                           tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                              >  2:1623738/1‑132 (MQ=255)                                                                                           tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                              <  1:1623738/132‑1 (MQ=255)                                                                                           tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                                      <  2:298071/140‑1 (MQ=255)                                                                                           tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                                      >  2:705908/1‑140 (MQ=255)                                                                                           tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                                      >  1:1062040/1‑140 (MQ=255)                                                                                              cGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                             <  2:1648142/130‑1 (MQ=255)                                                                                              cGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                             >  1:1648142/1‑130 (MQ=255)                                                                                                ggCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACtt                                 >  2:150114/1‑140 (MQ=255)                                                                                                  ccAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTAc                               <  1:742786/140‑1 (MQ=255)                                                                                                   cAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACg                              <  1:140964/140‑1 (MQ=255)                                                                                                          aCGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTc                       >  2:649184/1‑140 (MQ=255)                                                                                                           cGCTGGCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCa                      >  1:589074/1‑140 (MQ=255)                                                                                                                gCCCGCGGTTTCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAc                 >  1:135605/1‑140 (MQ=255)                                                                                                                          tCAGGGAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCg       >  1:763243/1‑140 (MQ=255)                                                                                                                               gAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTcc  <  1:548826/140‑1 (MQ=255)                                                                                                                               gAATGAGCAACGCTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTcc  <  2:955424/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 GCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCC  >  NC_000913/2171712‑2171977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑