Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,229,288	T→C	intergenic (+223/+150)	yohP → / ← dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,229,288	0	T	C	100.0%	131.4 / NA	46	intergenic (+223/+150)	yohP/dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/28);  total (18/28)

TAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAA  >  NC_000913/2229153‑2229420                                                                                                                                        |                                                                                                                                     taATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACa                                                                                                                                  <  2:456096/140‑1 (MQ=25)  aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACAt                                                                                                                                 <  2:634446/140‑1 (MQ=21)   aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATc                                                                                                                                <  2:1674405/140‑1 (MQ=18)         ggCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTa                                                                                                                          >  1:145911/1‑140 (MQ=17)          gcgcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTaa                                                                                                                         <  1:1313362/140‑1 (MQ=17)            gcAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCaa                                                                                                                                    >  2:566970/1‑127 (MQ=17)                    cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCATCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCt                                                                                                               <  1:1684523/140‑1 (MQ=17)                    cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCt                                                                                                               <  1:97900/140‑1 (MQ=17)                        cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGc                                                                                                           <  2:145911/140‑1 (MQ=17)                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                                                                                      <  2:1142054/140‑1 (MQ=17)                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                                                                                      <  2:1421078/140‑1 (MQ=17)                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCca                                                                                                        >  1:74149/1‑138 (MQ=17)                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCca                                                                                                        <  2:74149/138‑1 (MQ=17)                                 gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                                                                                                  >  1:1677538/1‑140 (MQ=17)                                  ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt                                                                                                 <  2:1045189/140‑1 (MQ=17)                                     aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                                                                                              >  2:569731/1‑140 (MQ=17)                                     aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAAAGCGCCCTCAGCCCACATCCAtgtg                                                                                              >  2:174931/1‑140 (MQ=17)                                     aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCCGTAAAAAGCGCCCCCAGCCCCCATCCAtgtg                                                                                              >  2:1288256/1‑140 (MQ=11)                                               cccccTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg                                                                                    <  1:1200391/140‑1 (MQ=17)                                                    tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt                                                                               >  2:507026/1‑140 (MQ=17)                                                           ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa                                                                        >  2:209267/1‑140 (MQ=17)                                                            cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACACCCATGTGGGCTGCCCCGGCCTTCAGGGAAc                                                                       >  1:1414079/1‑140 (MQ=17)                                                                         aCAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGGCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAAttt                                                          >  1:325556/1‑140 (MQ=21)                                                                            aaaTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGa                                                       <  2:1004410/140‑1 (MQ=21)                                                                               tCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGc                                                    <  1:1288256/140‑1 (MQ=25)                                                                                       gcgcGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAaac                                            >  1:1550193/1‑140 (MQ=37)                                                                                              gCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTg                                     <  1:569731/140‑1 (MQ=255)                                                                                              gCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTg                                     <  2:42483/140‑1 (MQ=255)                                                                                              gCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTg                                     <  1:112233/140‑1 (MQ=255)                                                                                                ccTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCg                                   <  2:1231312/140‑1 (MQ=255)                                                                                                  tCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGc                                 <  1:626192/140‑1 (MQ=255)                                                                                                   cGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGcc                                <  1:304984/140‑1 (MQ=255)                                                                                                   cGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGcc                                <  1:209267/140‑1 (MQ=255)                                                                                                   cGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACACCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGcc                                <  2:1414079/140‑1 (MQ=255)                                                                                                    ggCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCt                               <  2:325556/140‑1 (MQ=255)                                                                                                         aTCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGa                          >  2:88786/1‑140 (MQ=255)                                                                                                         aTCCCTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCCGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGa                          >  2:1250876/1‑140 (MQ=255)                                                                                                            ccTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATaa                       <  1:839786/140‑1 (MQ=255)                                                                                                            ccTTACGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATaa                       <  1:629906/140‑1 (MQ=255)                                                                                                                aCGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTa                              >  2:447113/1‑129 (MQ=255)                                                                                                                aCGGCTACCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTa                              <  1:447113/129‑1 (MQ=255)                                                                                                                      aCCGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAaca             >  1:335141/1‑140 (MQ=255)                                                                                                                       ccGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAacac            >  1:1101291/1‑140 (MQ=255)                                                                                                                        cGGGTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGGGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAAcaca           >  1:301297/1‑140 (MQ=255)                                                                                                                          ggTCGGGCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACAtt         <  1:507026/140‑1 (MQ=255)                                                                                                                              gggCGCGAGGCAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCa     <  1:88786/140‑1 (MQ=255)                                                                                                                                 cgcgAGGCAACATCCCTGTAAAACGCGCCCTCAGTCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCaaaa  >  1:1588643/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     TAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACGCCTCGGCAATTTTGACGCCACCAAACAACCGTGCGGCCTATTGATAAAGAGCTAACACATTGTCAAAA  >  NC_000913/2229153‑2229420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑