Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,307,443	T→C	Q391Q (CAA→CAG)	yojI ←	ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,307,443	0	T	C	100.0%	231.6 / NA	64	Q391Q (CAA→CAG)	yojI	ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/35);  total (29/35)

TATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTG  >  NC_000913/2307307‑2307578                                                                                                                                         |                                                                                                                                        tATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgctgt                                                                                                                                      >  1:1236259/1‑140 (MQ=255)      gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTcgcc                                                                                                                                 >  2:367768/1‑140 (MQ=255)                     gccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGcc                                                                                                                  <  1:1010251/140‑1 (MQ=255)                      ccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCa                                                                                                                 <  2:645413/140‑1 (MQ=255)                         gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATcc                                                                                                              >  1:1169836/1‑140 (MQ=255)                            aCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGc                                                                                                           <  1:1622461/140‑1 (MQ=255)                               tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAg                                                                                                        <  2:675472/140‑1 (MQ=255)                                      cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgc                                                                                                 <  2:1292401/140‑1 (MQ=255)                                      cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGCTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgc                                                                                                 <  2:1206138/140‑1 (MQ=255)                                       aGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgcc                                                                                                <  1:367768/140‑1 (MQ=255)                                       aGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgcc                                                                                                >  2:577765/1‑140 (MQ=255)                                        gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgccg                                                                                               <  1:1563619/140‑1 (MQ=255)                                        gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgccg                                                                                               >  1:12567/1‑140 (MQ=255)                                        gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgccg                                                                                               >  1:594465/1‑140 (MQ=255)                                        gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgc                                                                                                 >  2:709780/1‑138 (MQ=255)                                        gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcgc                                                                                                 <  1:709780/138‑1 (MQ=255)                                            cGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCttt                                                                                           <  1:250610/140‑1 (MQ=255)                                             gggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTtg                                                                                          <  2:1418356/140‑1 (MQ=255)                                              ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTtgt                                                                                         >  1:577326/1‑140 (MQ=255)                                              ctaacccGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTtgt                                                                                         >  2:682651/7‑140 (MQ=255)                                               gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTtgtg                                                                                        <  2:1527324/140‑1 (MQ=255)                                                ttAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTgg                                                                                       <  2:627930/140‑1 (MQ=255)                                                 tAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGc                                                                                      <  2:1191311/140‑1 (MQ=255)                                                   gCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTg                                                                                    >  1:751498/1‑140 (MQ=255)                                                        tttACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGc                                                                                      <  2:497609/133‑1 (MQ=255)                                                        tttACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGc                                                                                      >  1:497609/1‑133 (MQ=255)                                                        tttACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACa                                                                               <  2:248296/140‑1 (MQ=255)                                                          tACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAg                                                                             <  2:820869/140‑1 (MQ=255)                                                          tACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAg                                                                             <  2:1657523/140‑1 (MQ=255)                                                           aCCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGc                                                                            <  1:1646866/140‑1 (MQ=255)                                                                 cGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTca                                                                      >  1:1366264/1‑140 (MQ=255)                                                                 cGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTca                                                                      >  2:1330725/1‑140 (MQ=255)                                                                 cGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTca                                                                      <  2:12567/140‑1 (MQ=255)                                                                 cGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCAGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTca                                                                      <  1:335964/140‑1 (MQ=255)                                                                    ccacagcagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTcaaca                                                                   <  2:1169836/137‑1 (MQ=255)                                                                        agcagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATAAAAGCCCGTCAACAACAt                                                               >  2:1381877/1‑140 (MQ=255)                                                                          cagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCg                                                             <  2:71908/140‑1 (MQ=255)                                                                                     aCAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTcaac                                                                    >  1:314430/1‑122 (MQ=255)                                                                                     aCAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTcaac                                                                    <  2:314430/122‑1 (MQ=255)                                                                                     aCAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGAt                                                  >  1:929632/1‑140 (MQ=255)                                                                                          cAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTcc                                             >  2:798214/1‑140 (MQ=255)                                                                                           aGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCg                                            <  1:1381877/140‑1 (MQ=255)                                                                                             aCATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCt                                          >  2:1367032/1‑140 (MQ=255)                                                                                              cATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTa                                         <  1:969917/140‑1 (MQ=255)                                                                                              cATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTa                                         >  1:1210164/1‑140 (MQ=255)                                                                                                  ggTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGt                                     <  1:682651/140‑1 (MQ=255)                                                                                                      aaCACTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgcc                                 >  1:1266854/1‑140 (MQ=255)                                                                                                        cacTGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgc                               <  2:577326/140‑1 (MQ=255)                                                                                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                            >  2:885131/1‑140 (MQ=255)                                                                                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                            >  2:879392/1‑140 (MQ=255)                                                                                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                            <  2:1266854/140‑1 (MQ=255)                                                                                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                            >  2:1122656/1‑140 (MQ=255)                                                                                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                            >  1:992425/1‑140 (MQ=255)                                                                                                           tGCCGAAAACAGTTTGCGATAATCCTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                            >  2:1263238/1‑140 (MQ=255)                                                                                                            gCCGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAAt                           >  1:1074084/1‑140 (MQ=255)                                                                                                             ccGAAAACAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATc                          >  2:1013650/1‑140 (MQ=255)                                                                                                                  aaCAGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGaaa                     >  2:428810/1‑140 (MQ=255)                                                                                                                     aGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAAcag                  <  2:1366264/140‑1 (MQ=255)                                                                                                                     aGTTTGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAAcag                  <  2:1074084/140‑1 (MQ=255)                                                                                                                       tttGCGATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAAcagca                >  2:1408301/1‑140 (MQ=255)                                                                                                                            gATAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCg           <  2:1210164/140‑1 (MQ=255)                                                                                                                             aTAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGc          <  1:879392/140‑1 (MQ=255)                                                                                                                              tAATCTTCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGcc         <  1:1013650/140‑1 (MQ=255)                                                                                                                                    tCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGttt   <  2:594465/140‑1 (MQ=255)                                                                                                                                    tCCGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGttt   <  1:1263238/140‑1 (MQ=255)                                                                                                                                     ccGGCTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTg  >  1:1213544/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTG  >  NC_000913/2307307‑2307578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑