Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	290,698	T→A	Q152L (CAG→CTG)	insB‑3 ←	IS1 protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	290,698	0	T	A	100.0%	125.3 / NA	37	Q152L (CAG→CTG)	insB‑3	IS1 protein InsB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/14);  total (23/14)

GCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGT  >  NC_000913/290561‑290767                                                                                                                                          |                                                                      gCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCAtgcagc                                                                     >  2:249086/1‑140 (MQ=255)     agaACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCa                                                                 <  1:177868/140‑1 (MQ=255)       aaCATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCAcc                                                               <  2:26385/140‑1 (MQ=255)         cATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGa                                                             >  2:416829/1‑140 (MQ=255)                 ggCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGGTTTTgaga                                                     >  2:1134629/1‑140 (MQ=255)                 ggCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTgaga                                                     >  2:487454/1‑140 (MQ=255)                 ggCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTgaga                                                     <  2:1209824/140‑1 (MQ=255)                  gCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGaa                                                    <  1:1370060/140‑1 (MQ=255)                     tgctgcCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGa                                                 >  2:4944/1‑140 (MQ=255)                      gctgcCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGAc                                                >  1:1385153/1‑140 (MQ=255)                      gctgcCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGAc                                                >  2:1661573/1‑140 (MQ=255)                              acttcattacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACtt                                        <  1:487454/140‑1 (MQ=255)                                  cattacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGt                                    <  2:794512/140‑1 (MQ=255)                                   attacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGGTTTTGAGAACGACAGCGACTTCCGTc                                   <  1:1134629/140‑1 (MQ=255)                                     tacttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTccc                                 >  2:1366703/1‑140 (MQ=255)                                      acttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACt                                         <  1:778629/131‑1 (MQ=255)                                      acttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACt                                         >  2:778629/1‑131 (MQ=255)                                      acttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCa                                >  2:800750/1‑140 (MQ=255)                                      acttcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCa                                >  2:1022962/1‑140 (MQ=255)                                         tcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGcc                             <  1:4944/140‑1 (MQ=255)                                         tcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGcc                             >  1:563761/1‑140 (MQ=255)                                         tcaGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGcc                             >  1:1056878/1‑140 (MQ=255)                                          caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGc                              <  1:257830/138‑1 (MQ=255)                                          caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGc                              >  2:257830/1‑138 (MQ=255)                                          caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCg                            >  1:1640362/1‑140 (MQ=255)                                          caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCg                            >  1:155080/1‑140 (MQ=255)                                          caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCg                            >  2:687247/1‑140 (MQ=255)                                          caGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTCGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCg                            >  2:133037/1‑140 (MQ=255)                                               gTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCa                       >  2:690378/1‑140 (MQ=255)                                                  aTGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGt                    >  1:881615/1‑140 (MQ=255)                                                  aTGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGt                    >  2:347444/1‑140 (MQ=255)                                                  aTGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGt                    >  2:744047/1‑140 (MQ=255)                                                  aTGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGt                    >  1:328975/1‑140 (MQ=255)                                                         cGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCt             <  1:1661573/140‑1 (MQ=255)                                                         cGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCt             <  1:838571/140‑1 (MQ=255)                                                            tatatCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAg          <  2:1640362/140‑1 (MQ=38)                                                                   gTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTCGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAgg   <  1:133037/140‑1 (MQ=17)                                                                    ttACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGt  >  1:201585/1‑140 (MQ=14)                                                                                                                                          |                                                                      GCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGATGGAGGCGATATATCTGTTACCGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCTGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGT  >  NC_000913/290561‑290767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑