Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,776	G→A	A233V (GCG→GTG)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,776	0	G	A	100.0%	128.4 / NA	37	A233V (GCG→GTG)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/24);  total (13/24)

CGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAA  >  NC_000913/2446642‑2446909                                                                                                                                       |                                                                                                                                      cgAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATgg                                                                                                                                  >  1:1125079/1‑140 (MQ=255)  gagaATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATggg                                                                                                                                 <  2:230751/140‑1 (MQ=255)    gaATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCg                                                                                                                               <  2:1390246/140‑1 (MQ=255)    gaATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCg                                                                                                                               >  2:842495/1‑140 (MQ=255)        gccgccCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGt                                                                                                                           <  2:687527/140‑1 (MQ=255)            cccgcATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGc                                                                                                                       >  1:1176880/1‑140 (MQ=255)                   ggTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAgg                                                                                                                <  1:155646/140‑1 (MQ=255)                   ggTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAgg                                                                                                                <  2:1445657/140‑1 (MQ=255)                   ggTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAgg                                                                                                                <  1:842495/140‑1 (MQ=255)                       gCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGt                                                                                                            <  2:110148/140‑1 (MQ=255)                         tctGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCa                                                                                                          <  1:501505/140‑1 (MQ=255)                                   gTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCt                                                                                                >  1:1325723/1‑140 (MQ=255)                                        tGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCa                                                                                           <  1:836678/140‑1 (MQ=255)                                               ttCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCgg                                                                                    >  1:444312/1‑140 (MQ=255)                                                tCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGc                                                                                   <  1:317265/140‑1 (MQ=255)                                                  aTCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAAACCGGCTCGGCAAGGCCCGccc                                                                                 >  2:1119887/1‑138 (MQ=255)                                                          tCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCa                                                                         <  2:1416467/140‑1 (MQ=255)                                                           cTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCAc                                                                        >  1:620653/1‑140 (MQ=255)                                                               cTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGc                                                                    <  1:1118204/140‑1 (MQ=255)                                                                tGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGca                                                                   <  2:261936/140‑1 (MQ=255)                                                                 gCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGcaa                                                                  <  2:620653/140‑1 (MQ=255)                                                                     cgcAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCa                                                                         >  2:1002118/1‑129 (MQ=255)                                                                     cgcAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCa                                                                         <  1:1002118/129‑1 (MQ=255)                                                                      gcAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACg                                                             <  2:1158357/140‑1 (MQ=255)                                                                              accacGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACttt                                                     <  2:482024/140‑1 (MQ=255)                                                                                             tCGCCAATTTCTACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTc                                      >  1:1153938/1‑140 (MQ=255)                                                                                             tCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTc                                      >  1:274165/1‑140 (MQ=255)                                                                                               gCCAATTTCTACGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGc                                    <  2:1153938/140‑1 (MQ=255)                                                                                                          cGCCTTTCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGGGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTc                         >  1:556326/1‑140 (MQ=255)                                                                                                              tttCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAgcg                     <  2:1176880/140‑1 (MQ=255)                                                                                                               ttCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAgcgc                    <  2:67135/140‑1 (MQ=255)                                                                                                                tCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGc                                    >  1:958713/1‑123 (MQ=255)                                                                                                                tCACCGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGc                                    <  2:958713/123‑1 (MQ=255)                                                                                                                   ccGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGcgcacgc                <  2:274165/140‑1 (MQ=255)                                                                                                                   ccGCGTTGATGCTCATCAGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGcgcacgc                <  1:1119887/140‑1 (MQ=255)                                                                                                                               tcatcaGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCt    <  2:1325723/140‑1 (MQ=255)                                                                                                                                 atcaGCACATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTaa  >  1:1342772/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      CGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAGCGCCACCACGTCAAAGCCGTCGCCAATTTCCACGCCTTTCACCGCGTTGATGCTCATCAGCGCATGGGCGATGTCAGCATCCAGGCGGTCAAAGACCGGCTCGCCAAGTCCGGCAGGAACGCCACTGGCAACAACGGTGACTTTAGCGCCGATGGAGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAA  >  NC_000913/2446642‑2446909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑