Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,588,987	G→A	A465T (GCC→ACC)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,588,987	0	G	A	100.0%	307.9 / NA	93	A465T (GCC→ACC)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/53);  total (40/53)

GAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGC  >  NC_000913/2588854‑2589107                                                                                                                                      |                                                                                                                         gactgagGAAGGACCCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCt                                                                                                                    <  2:1041130/137‑1 (MQ=255)   gtgagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTAt                                                                                                                  >  1:1568158/1‑140 (MQ=255)    tgagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCAACTATc                                                                                                                 >  2:746919/1‑140 (MQ=255)    tgagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCCGCGGCACCACCGGTACCATCTATc                                                                                                                 >  2:884035/1‑140 (MQ=255)     gagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTAt                                                                                                                  <  1:1686077/138‑1 (MQ=255)     gagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTAt                                                                                                                  >  2:1686077/1‑138 (MQ=255)     gagGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCg                                                                                                                >  2:68460/1‑140 (MQ=255)      aggaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGc                                                                                                               <  2:1059444/140‑1 (MQ=255)      aggaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGc                                                                                                               >  2:1146981/1‑140 (MQ=255)      aggaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGc                                                                                                               >  1:168582/1‑140 (MQ=255)      aggaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGc                                                                                                               >  1:1281204/1‑140 (MQ=255)      aggaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGc                                                                                                               >  1:136558/1‑140 (MQ=255)       ggaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGcc                                                                                                              >  2:441270/1‑140 (MQ=255)        gaaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCa                                                                                                             >  1:1160757/1‑140 (MQ=255)         aaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAg                                                                                                            <  1:68460/140‑1 (MQ=255)         aaggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAg                                                                                                            <  1:1146981/140‑1 (MQ=255)           ggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGtt                                                                                                          <  2:837145/140‑1 (MQ=255)           ggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGtt                                                                                                          <  1:746919/140‑1 (MQ=255)           ggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGtt                                                                                                          <  1:789947/140‑1 (MQ=255)           ggaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGtt                                                                                                          <  2:4554/140‑1 (MQ=255)            gaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACTACCGGTACCATCTATCGCCAGTtc                                                                                                         <  2:864377/140‑1 (MQ=255)            gaCTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTtc                                                                                                         >  2:1177094/1‑140 (MQ=255)              ctcactcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTtctc                                                                                                       >  2:1194939/1‑140 (MQ=255)                cactcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTa                                                                                                     >  2:828070/1‑140 (MQ=255)                cactcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTa                                                                                                     <  1:1194939/140‑1 (MQ=255)                 actcCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTAt                                                                                                    <  1:554717/140‑1 (MQ=255)                  ctcctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTAtt                                                                                                   >  1:476560/1‑140 (MQ=255)                     ctcGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTAcc                                                                                                >  1:1442673/1‑140 (MQ=255)                        gcgAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGtt                                                                                                          >  1:139119/1‑127 (MQ=255)                        gcgAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGtt                                                                                                          <  2:139119/127‑1 (MQ=255)                         cgAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCAttg                                                                                            >  1:1099832/1‑140 (MQ=255)                             gCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgc                                                                                        >  1:534378/1‑140 (MQ=255)                             gCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgc                                                                                        >  1:275190/1‑140 (MQ=255)                                 cacGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgcggcg                                                                                    <  1:48512/140‑1 (MQ=255)                                 cacGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgcggcg                                                                                    >  1:1355094/1‑140 (MQ=255)                                 cacGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgcggcg                                                                                    >  1:53397/1‑140 (MQ=255)                                 cacGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgcggcg                                                                                    <  1:828070/140‑1 (MQ=255)                                      aaaTCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGt                                                                               <  2:1622429/140‑1 (MQ=255)                                      aaaTCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGt                                                                               <  2:1568158/140‑1 (MQ=255)                                      aaaTCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGt                                                                               <  2:628912/140‑1 (MQ=255)                                      aaaTCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGt                                                                               <  2:1632435/140‑1 (MQ=255)                                         tCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCt                                                                            <  2:534378/140‑1 (MQ=255)                                         tCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCt                                                                            <  1:1102659/140‑1 (MQ=255)                                         tCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCt                                                                            <  1:1177094/140‑1 (MQ=255)                                         tCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCt                                                                            <  2:1442673/140‑1 (MQ=255)                                         tCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCNATCGCCAGTTCTNTATTACCATTGTTGCGGCGATGGTGCt                                                                            <  2:1258409/140‑1 (MQ=255)                                          cGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTg                                                                           <  2:275190/140‑1 (MQ=255)                                           gATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCAGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGt                                                                          <  2:1225360/140‑1 (MQ=255)                                            aTGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTc                                                                         <  2:476560/140‑1 (MQ=255)                                            aTGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTc                                                                         >  1:223179/1‑140 (MQ=255)                                              ggggCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAg                                                                       >  2:1049664/1‑140 (MQ=255)                                               gggCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGt                                                                      <  1:312802/140‑1 (MQ=255)                                               gggCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTA‑TACCATTGTTGCGGCGATGGTGCTGTCAGTa                                                                     <  1:260357/140‑1 (MQ=255)                                                ggCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgcggcg                                                                                    <  1:397313/125‑1 (MQ=255)                                                ggCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTgcggcg                                                                                    >  2:397313/1‑125 (MQ=255)                                                ggCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTa                                                                     >  2:265168/1‑140 (MQ=255)                                                  cAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACt                                                                   >  1:993890/1‑140 (MQ=255)                                                       ccAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAg                                                              <  2:1281204/140‑1 (MQ=255)                                                         aGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCg                                                            <  2:993890/140‑1 (MQ=255)                                                           ggggCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCgat                                                          <  2:1681741/140‑1 (MQ=255)                                                           ggggCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCgat                                                          <  1:687845/140‑1 (MQ=255)                                                            gggCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCgatg                                                         >  1:279211/1‑140 (MQ=255)                                                               cACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTTTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATc                                                      >  2:720056/1‑140 (MQ=255)                                                               cACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATc                                                      >  2:492353/1‑140 (MQ=255)                                                               cACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATc                                                      >  2:419835/1‑140 (MQ=255)                                                                aCTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATcc                                                     >  2:1284923/1‑140 (MQ=255)                                                                aCTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATcc                                                     >  2:324332/1‑140 (MQ=255)                                                                 cTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCt                                                    <  1:1328720/140‑1 (MQ=255)                                                                 cTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCt                                                    <  2:74473/140‑1 (MQ=255)                                                                 cTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCt                                                    <  2:683123/140‑1 (MQ=255)                                                                 cTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCt                                                    <  1:265168/140‑1 (MQ=255)                                                                      cGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTc                                               <  1:1049664/140‑1 (MQ=255)                                                                           ttGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGct                                          <  2:53397/140‑1 (MQ=255)                                                                            tGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGctc                                         >  2:1552524/1‑140 (MQ=255)                                                                            tGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGctc                                         >  2:1058211/1‑140 (MQ=255)                                                                             gCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGctct                                        <  1:593710/140‑1 (MQ=255)                                                                                  ggTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCtgtgtg                                   <  2:1355094/140‑1 (MQ=255)                                                                                  ggTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCtgtgtg                                   <  1:1391135/140‑1 (MQ=255)                                                                                  ggTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCtgtgtg                                   <  1:324332/140‑1 (MQ=255)                                                                                   gTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGc                                  >  1:86982/1‑140 (MQ=255)                                                                                     tCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCca                                <  2:86982/140‑1 (MQ=255)                                                                                       tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCcaca                              <  1:1284923/140‑1 (MQ=255)                                                                                       tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCcaca                              <  1:1552524/140‑1 (MQ=255)                                                                                       tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCca                                <  1:467209/138‑1 (MQ=255)                                                                                       tttCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCca                                >  2:467209/1‑138 (MQ=255)                                                                                             cggTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACtt                        >  1:689114/1‑140 (MQ=255)                                                                                               gTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTaa                      >  2:1365253/1‑140 (MQ=255)                                                                                                tATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAg                     >  2:1138864/1‑140 (MQ=255)                                                                                                   ttGTACCAATGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCa                  <  1:1365253/140‑1 (MQ=255)                                                                                                          aaTGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGaaaa           <  2:1160757/140‑1 (MQ=255)                                                                                                            tGGCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGaaaaaa         >  2:1048308/1‑140 (MQ=255)                                                                                                             ggCCTTCTTCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAg        <  1:720056/140‑1 (MQ=255)                                                                                                                cttcttCGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTg     <  1:492353/140‑1 (MQ=255)                                                                                                                   cttcGGCGGCACCACCGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGc  >  1:248465/1‑140 (MQ=255)                                                                                                                              ccaccGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGa    <  1:399907/127‑1 (MQ=255)                                                                                                                              ccaccGGTACCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGa    >  2:399907/1‑127 (MQ=255)                                                                                                                                      |                                                                                                                         GAGTGAGGAAGGACTCACTCCTCGCGAAGCCACACGTAAATCGATGGGGCAGATCCAGGGGGCACTGGTCGGGATTGCGATGGTTCTTTCGGCGGTATTTGTACCAATGGCCTTCTTCGGCGGCACCACCGGTGCCATCTATCGCCAGTTCTCTATTACCATTGTTGCGGCGATGGTGCTGTCAGTACTGGTAGCGATGATCCTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGC  >  NC_000913/2588854‑2589107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑