Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,603,299	G→A	W494* (TGG→TGA)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,603,299	0	G	A	100.0%	264.7 / NA	74	W494* (TGG→TGA)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/28);  total (46/28)

AAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCT  >  NC_000913/2603163‑2603424                                                                                                                                         |                                                                                                                              aaaTGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTtgactg                                                                                                                            <  1:230458/140‑1 (MQ=255) aaaTGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTtgactg                                                                                                                            <  2:397406/140‑1 (MQ=255)      tACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCGcc                                                                                                                       <  2:1142617/140‑1 (MQ=255)      tACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCAcc                                                                                                                       >  1:1697802/1‑140 (MQ=255)      tACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCAcc                                                                                                                       <  2:891602/140‑1 (MQ=255)      tACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCAcc                                                                                                                       <  2:672806/140‑1 (MQ=255)      tACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCAcc                                                                                                                       >  1:1469683/1‑140 (MQ=255)       aCGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCg                                                                                                                      <  2:1469683/140‑1 (MQ=255)       aCGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCg                                                                                                                      <  2:1669646/140‑1 (MQ=255)       aCGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCg                                                                                                                      >  2:40047/1‑140 (MQ=255)        cGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGa                                                                                                                     <  2:1226477/140‑1 (MQ=255)          gTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAg                                                                                                                   <  1:371656/140‑1 (MQ=255)           tATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGa                                                                                                                     >  2:314666/1‑137 (MQ=255)           tATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGa                                                                                                                     <  1:314666/137‑1 (MQ=255)           tATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGa                                                                                                                  >  2:1085656/1‑140 (MQ=255)            aTTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGAt                                                                                                                 >  2:576162/1‑140 (MQ=255)             ttACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCTCTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATa                                                                                                                <  2:265003/140‑1 (MQ=255)               aCTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAAt                                                                                                              <  2:202804/140‑1 (MQ=255)                    cTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCata                                                                                                         <  1:576162/140‑1 (MQ=255)                    cTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCata                                                                                                         <  1:765767/140‑1 (MQ=255)                    cTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCata                                                                                                         >  1:403182/1‑140 (MQ=255)                     tgtgGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATaa                                                                                                               <  2:971761/133‑1 (MQ=255)                     tgtgGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATaa                                                                                                               >  1:971761/1‑133 (MQ=255)                     tgtgGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCatat                                                                                                        >  1:609595/1‑140 (MQ=255)                     tgtgGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCatat                                                                                                        >  1:542893/1‑140 (MQ=255)                     tgtgGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCatat                                                                                                        >  1:1492292/1‑140 (MQ=255)                     tgtgGTGCGCCGCGCAGTACACACGCAGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCatat                                                                                                        >  1:1612005/1‑140 (MQ=255)                     tgtgGTGCGCCGCGCAGCACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCatat                                                                                                        >  1:898402/1‑140 (MQ=255)                       tggtgCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTg                                                                                                      >  1:407349/1‑140 (MQ=255)                       tggtgCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGCGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGGATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTg                                                                                                      >  1:479354/1‑140 (MQ=255)                          tgCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAg                                                                                                                   <  2:1143078/124‑1 (MQ=255)                          tgCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAg                                                                                                                   >  1:1143078/1‑124 (MQ=255)                          tgCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGccc                                                                                                   <  1:1584042/140‑1 (MQ=255)                          tgCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGccc                                                                                                   <  1:577303/140‑1 (MQ=255)                           gcgcCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCa                                                                                                  <  1:1085656/140‑1 (MQ=255)                            cgccgcGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCAt                                                                                                 >  1:57174/1‑140 (MQ=255)                            cgccgcGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCAt                                                                                                 >  1:188811/1‑140 (MQ=255)                            cgccgcGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCAt                                                                                                 >  1:679681/1‑140 (MQ=255)                              ccgcGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGccc                                                                                                   <  1:650093/136‑1 (MQ=255)                              ccgcGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGccc                                                                                                   >  2:650093/1‑136 (MQ=255)                               cgcgCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCg                                                                                              <  2:702700/140‑1 (MQ=255)                               cgcgCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCg                                                                                              <  1:40047/140‑1 (MQ=255)                                gcgcAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGt                                                                                             <  2:621937/140‑1 (MQ=255)                                 cgcAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGccc                                                                                                   <  1:1083222/133‑1 (MQ=255)                                 cgcAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGccc                                                                                                   >  2:1083222/1‑133 (MQ=255)                                 cgcAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGtt                                                                                            <  2:407349/140‑1 (MQ=255)                                 cgcAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGtt                                                                                            >  1:62535/1‑140 (MQ=255)                                    aGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTAc                                                                                         <  2:1659664/140‑1 (MQ=255)                                        cacacGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAAt                                                                                     >  1:583587/1‑140 (MQ=255)                                           acGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATAcc                                                                                  <  2:188811/140‑1 (MQ=255)                                           acGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATAcc                                                                                  <  2:679681/140‑1 (MQ=255)                                           acGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATAcc                                                                                  <  1:986551/140‑1 (MQ=255)                                              cTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCt                                                                               >  1:1317719/1‑140 (MQ=255)                                                gAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTcc                                                                             >  1:682331/1‑140 (MQ=255)                                                gAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTcc                                                                             >  1:1495605/1‑140 (MQ=255)                                                       cacaGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACt                                                                      >  1:1063068/1‑140 (MQ=255)                                                                tGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGc                                                             >  1:1170805/1‑140 (MQ=255)                                                                 gCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCg                                                            >  1:1359398/1‑140 (MQ=255)                                                                     aaTACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAAt                                                        >  1:1060823/1‑140 (MQ=255)                                                                      aTACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATa                                                       >  1:1495796/1‑140 (MQ=255)                                                                      aTACGATCACCGCCCCCTTGCTCCTCCCCGCGCCCCTCTGTTTATTAATGGCGTTTGGTCCCAGTTGACTGCCACAAAAAATAATGCATTTCCCCTATGTTTTCCCAAACCCCCCCCCCGCCACTGTCGCCAGCGGAATa                                                       <  2:542893/140‑1 (MQ=255)                                                                            tgatCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACtt                                                 >  1:823034/1‑140 (MQ=255)                                                                                 gtcgCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTAcc                                            >  1:88022/1‑140 (MQ=255)                                                                                    gCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCgg                                         >  1:257253/1‑140 (MQ=255)                                                                                     ccATGCTACCGCCCGCGCCACTCTGTTTATTATTTGCGTTTAGTCCCTTTTGACGGCCACAAAAAATAATGAATATTCCCCATGTTTTTACAACCCCCCCCCCCGCCACTGTCGCCAGTGGAATAGCACTTGTACCCGGc                                        <  2:583587/140‑1 (MQ=255)                                                                                       ttGCTCCTGCCCGCGCCTCTCTGTTTATTATTTGCGTTTAGTGCCAGTTGACGGCCACAAAAAATAATGCATTTTCCCCATGTTTTTCCAAACCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCAc                                      <  2:1356619/139‑1 (MQ=255)                                                                                        tGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACg                                     >  1:805601/1‑140 (MQ=255)                                                                                                  cgcgGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                           >  1:1356619/1‑140 (MQ=255)                                                                                                  cgcgGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATacac                           >  1:1432451/1‑140 (MQ=255)                                                                                                    cgGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAg                         >  1:88106/1‑140 (MQ=255)                                                                                                       cACTCTGTTTATTAATGGCGTTTGGCGCCAGTGGCCTGCCCCGAAAAATAATGAATATTGCCCTTGCTTTTCCCACCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTc                      <  2:88022/140‑1 (MQ=255)                                                                                                           ttgttttttATTTGCGCTTAGTGCGAGTTGACGGCCACAAAAAATAATGCTTATCCCCCCTGCTTTTCCCACCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCAccc                  <  2:57174/133‑1 (MQ=255)                                                                                                                      ttGCGCTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTg       >  1:889143/1‑140 (MQ=255)                                                                                                                        gcgcTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGtt     >  2:577873/1‑140 (MQ=255)                                                                                                                        gcgcTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGtt     >  1:1251753/1‑140 (MQ=255)                                                                                                                         cgcTTAGTGCTAGTTGACTGCCAACAAAAATAATGTATATGGCCCTTGTTTTTCCCACCCCCCCCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTg    <  2:682331/140‑1 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCt  >  1:1452587/1‑140 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCt  >  1:1467682/1‑140 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCt  >  1:412730/1‑140 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCt  >  1:47811/1‑140 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCt  >  1:712117/1‑140 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCAACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCAc                    <  1:1208717/122‑1 (MQ=255)                                                                                                                           cTTAGTGCCAGTTGACTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCAACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCAc                    >  2:1208717/1‑122 (MQ=255)                                                                                                                                         |                                                                                                                              AAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCATATTGCCCATGCGTTTACCAATACCCCTCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCT  >  NC_000913/2603163‑2603424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑