Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,610,630	T→C	V156A (GTC→GCC)	hyfH →	hydrogenase 4 component H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,610,630	0	T	C	100.0%	271.7 / NA	75	V156A (GTC→GCC)	hyfH	hydrogenase 4 component H
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/42);  total (33/42)

AACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCA  >  NC_000913/2610507‑2610758                                                                                                                            |                                                                                                                                 aaCGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCaaa                                                                                                                  >  2:1181127/1‑140 (MQ=255) aaCGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCaaa                                                                                                                  >  2:431711/1‑140 (MQ=255) aaCGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAACGAGCGCCTGCCCGGAATGCaaa                                                                                                                  >  2:376559/1‑140 (MQ=255)   cGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaaca                                                                                                                <  1:431711/140‑1 (MQ=255)   cGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaaca                                                                                                                >  2:102136/1‑140 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCTTCCCAGGGCCTGCTGAATTTTAAGCACAGCAACAAAATCCGCCACAAAACCGCGAAATGTTGTGGCCGCAAGCGAGCGCCTGCCGGGAATGCAaaaaacc                                                                                                             >  2:264566/1‑139 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGccc                                                                                                                            >  2:1671124/1‑125 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGccc                                                                                                                            <  1:1671124/125‑1 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcg                                                                                                             >  1:1646048/1‑140 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcg                                                                                                             >  1:536532/1‑140 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcg                                                                                                             >  1:986367/1‑140 (MQ=255)      tGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcg                                                                                                             >  2:133653/1‑140 (MQ=255)        cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCCTCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaaca                                                                                                                <  2:1567939/135‑1 (MQ=255)        cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAaaca                                                                                                                >  1:1567939/1‑135 (MQ=255)        cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg                                                                                                           >  2:119538/1‑140 (MQ=255)        cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg                                                                                                           >  2:277398/1‑140 (MQ=255)        cAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg                                                                                                           <  1:245440/140‑1 (MQ=255)           ccGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGa                                                                                                        <  2:241850/140‑1 (MQ=255)           ccGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGa                                                                                                        <  1:1181127/140‑1 (MQ=255)             gTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg                                                                                                           <  1:736485/135‑1 (MQ=255)             gTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAAcgcg                                                                                                           >  2:736485/1‑135 (MQ=255)             gTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACg                                                                                                      <  2:600102/140‑1 (MQ=255)              ttGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGc                                                                                                     <  1:133653/140‑1 (MQ=255)                   aaCGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATc                                                                                                <  2:1646048/140‑1 (MQ=255)                      gCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAac                                                                                             <  1:212771/140‑1 (MQ=255)                      gCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAac                                                                                             <  1:87580/140‑1 (MQ=255)                        ccGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga                                                                                           <  1:294890/140‑1 (MQ=255)                        ccGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAACGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAacga                                                                                           <  1:376559/140‑1 (MQ=255)                           tttGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAAcgacga                                                                                        <  1:519405/140‑1 (MQ=255)                            ttGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGAt                                                                                       <  2:1191408/140‑1 (MQ=255)                              gCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATAc                                                                                     <  1:219946/140‑1 (MQ=255)                                cccGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAg                                                                                   <  1:119538/140‑1 (MQ=255)                                  cGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGAt                                                                                 >  2:1291830/1‑140 (MQ=255)                                   gCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATg                                                                                <  1:277398/140‑1 (MQ=255)                                     aaaaaaCCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTa                                                                              <  1:1291830/140‑1 (MQ=255)                                          aCCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTActgct                                                                         <  1:65985/140‑1 (MQ=255)                                           ccATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTActgctg                                                                        <  2:595181/140‑1 (MQ=255)                                            cATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCtgg                                                                       <  1:638310/140‑1 (MQ=255)                                                  aCTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTa                                                                 <  1:1274789/140‑1 (MQ=255)                                                   cTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTaa                                                                >  1:1328030/1‑140 (MQ=255)                                                    tGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAg                                                               <  1:1013511/140‑1 (MQ=255)                                                    tGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAg                                                               <  2:1029011/140‑1 (MQ=255)                                                     ggCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGTGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAgg                                                              >  2:1440479/1‑140 (MQ=255)                                                      gctgctGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGa                                                             <  1:891783/140‑1 (MQ=255)                                                      gctgctGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGa                                                             >  2:17568/1‑140 (MQ=255)                                                       ctgctgAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGTGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGag                                                            <  1:1440479/140‑1 (MQ=255)                                                        tgctgAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGagc                                                           <  1:585689/140‑1 (MQ=255)                                                             aaTTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTa                                                      <  1:1039509/140‑1 (MQ=255)                                                              aTTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt                                                     <  1:874474/140‑1 (MQ=255)                                                              aTTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt                                                     <  1:17568/140‑1 (MQ=255)                                                                   tAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTc                                                <  2:63555/140‑1 (MQ=255)                                                                    aGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTcc                                               <  1:102136/140‑1 (MQ=255)                                                                    aGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTcc                                               >  1:1281638/1‑140 (MQ=255)                                                                         aGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt                                                     >  2:318611/1‑129 (MQ=255)                                                                         aGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTAt                                                     <  1:318611/129‑1 (MQ=255)                                                                         aGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGc                                          >  1:317741/1‑140 (MQ=255)                                                                          gCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCt                                         >  1:1429360/1‑140 (MQ=255)                                                                           caacaaAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCtt                                        >  1:1071857/1‑140 (MQ=255)                                                                           caacaaAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCtt                                        >  1:416420/1‑140 (MQ=255)                                                                           caacaaAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCtt                                        >  1:287651/1‑140 (MQ=255)                                                                            aacaaAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTa                                       >  2:925695/1‑140 (MQ=255)                                                                                aaaTGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTAcaca                                   <  1:286706/140‑1 (MQ=255)                                                                                aaaTGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTAcaca                                   >  1:205980/1‑140 (MQ=255)                                                                                   tGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACacaaca                                >  2:21625/1‑140 (MQ=255)                                                                                   tGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACacaaca                                >  2:1238005/1‑140 (MQ=255)                                                                                     cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATg                              >  2:431670/1‑140 (MQ=255)                                                                                     cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATg                              >  1:1376420/1‑140 (MQ=255)                                                                                     cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATg                              >  2:648604/1‑140 (MQ=255)                                                                                     cgcCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATg                              >  1:1069353/1‑140 (MQ=255)                                                                                       ccACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTc                            <  1:648604/140‑1 (MQ=255)                                                                                             aaCCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTcagccag                      <  2:205980/140‑1 (MQ=255)                                                                                             aaCCGCGAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTcagccag                      <  1:431670/140‑1 (MQ=255)                                                                                                  cgAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCAt                 <  2:416420/140‑1 (MQ=255)                                                                                                  cgAAATGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCAt                 <  2:1140950/140‑1 (MQ=255)                                                                                                      aTGTTGTGGGCGCAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACg             <  2:1376420/140‑1 (MQ=255)                                                                                                                 gcAAGCGAGCGCCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCa  >  2:890845/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                 AACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCATCGCACTGGCTGCTGAATTGTTAGCACAGCAACAAAATGCGCCACAAAACCGCGAAATGTTGTGGGCGCAAGCGAGCGTCTGCCCGGAATGCAAACAACGCGCGACGCTGATCAACGACGATACAGATGTACTGCTGGTGGCTAAGGAGCAGCTATGAGTCCAGTGCTTACACAACATGTCAGCCAGCCCATCACGCTGGACGAGCA  >  NC_000913/2610507‑2610758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑