Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,602	A→G	M244T (ATG→ACG)	ecpC ←	putative fimbrial usher protein EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,602	0	A	G	100.0%	286.5 / NA	83	M244T (ATG→ACG)	ecpC	putative fimbrial usher protein EcpC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/54);  total (29/54)

ATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCATTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAG  >  NC_000913/308470‑308731                                                                                                                                     |                                                                                                                                  aTCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCAcc                                                                                                                            >  2:865794/1‑140 (MQ=255) aTCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCCAAGGACTGCAAGTTCCAGGGGGCGAGCGTTCCAcc                                                                                                                            >  2:581644/1‑140 (MQ=255)               tGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGAc                                                                                                              <  1:690821/140‑1 (MQ=255)               tGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGAc                                                                                                              <  2:702028/140‑1 (MQ=255)                   tGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGc                                                                                                          <  1:938741/140‑1 (MQ=255)                      ctgctgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATAGGTGACCGGCaaa                                                                                                       <  1:141486/140‑1 (MQ=255)                       tgctgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCaaaa                                                                                                      >  2:437293/1‑140 (MQ=255)                        gctgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAAt                                                                                                     <  1:865794/140‑1 (MQ=255)                        gctgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAAt                                                                                                     <  1:408502/140‑1 (MQ=255)                        gctgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAAt                                                                                                     <  2:356941/140‑1 (MQ=255)                          tgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATcg                                                                                                   <  2:558376/140‑1 (MQ=255)                          tgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATcg                                                                                                   <  1:581644/140‑1 (MQ=255)                          tgTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATcg                                                                                                   <  2:389278/140‑1 (MQ=255)                            tCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATcgcg                                                                                                 <  1:1358134/140‑1 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATcg                                                                                                   <  1:1101922/128‑1 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATcg                                                                                                   >  2:1101922/1‑128 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACa                                                                                         <  1:1535694/138‑1 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACa                                                                                         >  2:1535694/1‑138 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATc                                                                                       >  1:1046486/1‑140 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATc                                                                                       >  1:998740/1‑140 (MQ=255)                                      tggAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCAGCAAAATCGCGTTCATACATc                                                                                       >  2:949604/1‑140 (MQ=255)                                         aGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    <  2:356445/140‑1 (MQ=255)                                         aGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    <  1:437293/140‑1 (MQ=255)                                         aGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    <  1:495799/140‑1 (MQ=255)                                         aGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    <  2:1421667/140‑1 (MQ=255)                                         aGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    <  2:578240/140‑1 (MQ=255)                                           cTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCttt                                                                                  <  1:1240932/140‑1 (MQ=255)                                           cTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCttt                                                                                  <  2:808623/140‑1 (MQ=255)                                           cTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCttt                                                                                  >  1:202498/1‑140 (MQ=255)                                           cTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCttt                                                                                  <  2:1046486/140‑1 (MQ=255)                                             ggCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    >  1:597182/1‑136 (MQ=255)                                             ggCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCt                                                                                    <  2:597182/136‑1 (MQ=255)                                              gCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTtata                                                                               >  1:188471/1‑140 (MQ=255)                                              gCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTtata                                                                               >  1:1317198/1‑140 (MQ=255)                                               ccTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTtatat                                                                              <  1:1325797/140‑1 (MQ=255)                                                cTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTtatata                                                                             <  2:998740/140‑1 (MQ=255)                                                cTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTtatata                                                                             <  1:873681/140‑1 (MQ=255)                                                cTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCAGCAAAATCGCGTTCATACATCGCTTtatata                                                                             <  1:949604/140‑1 (MQ=255)                                                     ttCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCa                                                                        <  2:1317198/140‑1 (MQ=255)                                                        cccAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTg                                                                     >  1:860378/1‑140 (MQ=255)                                                        cccAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTg                                                                     >  2:939768/1‑140 (MQ=255)                                                          cAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTc                                                                   <  1:735922/140‑1 (MQ=255)                                                          cAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCCGCGCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTc                                                                   <  1:1563433/140‑1 (MQ=255)                                                             gAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTg                                                                >  2:698232/1‑140 (MQ=255)                                                                 ggCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGa                                                            <  2:202498/140‑1 (MQ=255)                                                                  gCCGTAAATCTTCCCTGCTGAAATGGTGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGAc                                                           <  1:617164/140‑1 (MQ=255)                                                                  gCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTg                                                                >  2:639112/1‑135 (MQ=255)                                                                  gCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTg                                                                <  1:639112/135‑1 (MQ=255)                                                                  gCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGAc                                                           <  2:860378/140‑1 (MQ=255)                                                                  gCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGAc                                                           <  2:139113/140‑1 (MQ=255)                                                                        aaTCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTAc                                                     <  2:631681/140‑1 (MQ=255)                                                                            ttCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGAt                                                 >  1:236040/1‑140 (MQ=255)                                                                                ctgctgAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCg                                             <  1:1156295/140‑1 (MQ=255)                                                                                   ctgAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTAc                                          >  2:1626418/1‑140 (MQ=255)                                                                                      aaaTGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTAc                                                     <  2:858392/126‑1 (MQ=255)                                                                                      aaaTGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTAc                                                     >  1:858392/1‑126 (MQ=255)                                                                                             ggTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGt                                >  2:226010/1‑140 (MQ=255)                                                                                                cATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGa                             <  2:1367537/140‑1 (MQ=255)                                                                                                 aTCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAg                            <  2:236040/140‑1 (MQ=255)                                                                                                 aTCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAg                            <  1:698232/140‑1 (MQ=255)                                                                                                  tCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGc                           <  1:226010/140‑1 (MQ=255)                                                                                                  tCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGc                           <  1:1626418/140‑1 (MQ=255)                                                                                                        cTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCg                                 <  1:712610/128‑1 (MQ=255)                                                                                                        cTAAGGACTGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCg                                 >  2:712610/1‑128 (MQ=255)                                                                                                                tGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTc             >  2:1547615/1‑140 (MQ=255)                                                                                                                tGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTc             >  1:967844/1‑140 (MQ=255)                                                                                                                tGCAAGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTc             >  2:660581/1‑140 (MQ=255)                                                                                                                  cAAGTTCCAGGGGGCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgc           >  2:1435745/1‑140 (MQ=255)                                                                                                                   aaGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgcg          <  2:188471/140‑1 (MQ=255)                                                                                                                   aaGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgcg          <  2:967844/140‑1 (MQ=255)                                                                                                                   aaGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgcg          <  1:235509/140‑1 (MQ=255)                                                                                                                   aaGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgcg          <  2:1402582/140‑1 (MQ=255)                                                                                                                    aGTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCgcgc         <  1:1134501/140‑1 (MQ=255)                                                                                                                     gTTCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgca        >  1:1093291/1‑140 (MQ=255)                                                                                                                      ttCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcag       <  1:693456/140‑1 (MQ=255)                                                                                                                       tCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGTTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgcg          >  1:1563300/1‑136 (MQ=255)                                                                                                                       tCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGTTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTcgcg          <  2:1563300/136‑1 (MQ=255)                                                                                                                       tCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcagt      >  1:643882/1‑140 (MQ=255)                                                                                                                       tCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcagt      >  2:891525/1‑140 (MQ=255)                                                                                                                       tCCAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcagt      >  1:377727/1‑140 (MQ=255)                                                                                                                        ccAGGTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcagtg     <  1:1547615/140‑1 (MQ=255)                                                                                                                           ggTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcagtgcag  <  1:1286624/140‑1 (MQ=255)                                                                                                                           ggTGTCGAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcagtgcag  <  1:939768/140‑1 (MQ=255)                                                                                                                                 gAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcag       <  1:1206956/129‑1 (MQ=255)                                                                                                                                 gAGCGTTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCgcag       >  2:1206956/1‑129 (MQ=255)                                                                                                                                     |                                                                                                                                  ATCACTGGCGTGGCTGACTGGCTGCTGTCGAAGATGGTGGAGCTGGCCTGGTTTCCCCAGGAAAGGCCGTAAATCTTCCCTGCTGAAATGGCGGTCATCGGCCCTAAGGACTGCAAGTTCCAGGTGTCGAGCATTCCACCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAG  >  NC_000913/308470‑308731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑